Cloned (Comment) | Organism |
---|---|
a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases expressed in Escherichia coli | Luciola cruciata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Luciola cruciata | - |
- |
- |
Photinus pyralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases by ammonium sulfate precipitation, gel filtration and hydroxyapatite column chromatography | Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases is more stable than the wild type enzyme, retaining 75% of the activity after 10 min at 50°C | Luciola cruciata |