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Literature summary for 1.13.12.7 extracted from
- Firefly luciferase (1976), Adv. Enzymol. Relat. Areas Mol. Biol., 44, 37-68.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | activator of yellow-green light at pH 6.5 | Photinus pyralis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole | competitive inhibitor | Photinus pyralis | |
| 5-anilino-1-naphthalene sulfonate | - |
Photinus pyralis | |
| 6-toluidino-2-naphthalene sulfonate | - |
Photinus pyralis | |
| ATP | uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex | Photinus pyralis | |
| dehydroluciferin | - |
Photinus pyralis | |
| N-ethylmaleimide | - |
Photinus pyralis | |
| N-tosyl-L-phenylalanine chloromethyl ketone | - |
Photinus pyralis | |
| p-mercuribenzoate | - |
Photinus pyralis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | increasing concentration shifts the light wavelenghts to higher values | Photinus pyralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
- |
Photinus pyralis |
| 92000 | 95000 | high speed equilibrium sedimentation | Photinus pyralis |
| 100000 | - |
from sedimentation velocity of the crystalline form | Photinus pyralis |
| 500000 | - |
2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme | Photinus pyralis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Photinus luciferin + O2 + ATP | Photinus pyralis | highly specific for ATP | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photinus pyralis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quantum yield 0.88 with respect to luciferin | Photinus pyralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dimethylluciferin + ATP + O2 | produces only red light, 6-amino and 4 hydroxy analogs tested | Photinus pyralis | dehydroluciferin-adenylate + diphosphate | - |
? | |
| dehydroluciferin + ATP + O2 | - |
Photinus pyralis | diphosphate + dehydroluciferin-adenylate | - |
? | |
| luciferin + ATP + O2 | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
r | |
| luciferin + MgATP2- | - |
Photinus pyralis | AMP + diphosphate | - |
? | |
| Photinus luciferin + O2 + ATP | highly specific for ATP | Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
| Photinus luciferin + O2 + ATP | the true substrate is MgATP2- | Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | two non identical subunits | Photinus pyralis |
| dimer | 2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme | Photinus pyralis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | pH up to 8 red light occurs instead of yellow-green light | Photinus pyralis |
| 7.5 | 8 | pH below 7.5 emission shifts to longer wavelengths | Photinus pyralis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00001 | - |
dehydroluciferin | - |
Photinus pyralis | |
| 0.0006 | - |
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole | - |
Photinus pyralis | |
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