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Literature summary for 1.13.12.7 extracted from

  • Webster, J.J.; Leach, F.R.
    Optimization of the firefly luciferase assay for ATP (1980), J. Appl. Biochem., 2, 469-479.
No PubMed abstract available
Search for more literature for 1.13.12.7

Activating Compound

Activating Compound Comment Organism Structure
Luciferin preparations supplemented with extra luciferin provide maximum light emission Photinus pyralis

General Stability

General Stability Organism
bovine serum albumin stabilizes Photinus pyralis
dithiothreitol stabilizes Photinus pyralis
EDTA stabilizes Photinus pyralis
more stable in phosphate buffer than in Tricine buffer Photinus pyralis

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
 Photinus pyralis
EDTA
-
 Photinus pyralis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Photinus pyralis
Mg2+ concentration 5 mM, MgSO4, MgCl2, Mg(O2CCH3)2 equally suitable salts Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP the true substrate is MgATP2- Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Photinus pyralis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 buffers with pKa from 7.2 to 8.4 tested and no relationship with activity found Photinus pyralis
6.5 8.5 pH 6.5: about 45% of activity maximum, pH 8.5: about 35% of activity maximum Photinus pyralis