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Literature summary for 1.13.12.7 extracted from
- The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps (2005), FEBS Lett., 579, 4389-4394.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL10-Gold cells | Photinus pyralis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H245D | longest rise time known among single point mutants | Photinus pyralis |
| K529A | similar rise time compared to wild type enzyme | Photinus pyralis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | enzyme activity without 10 mM MgSO4 decreases to about 50% of that of wild type enzyme in the presence of Mg2+ | Photinus pyralis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photinus pyralis | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 50 mM Tris-HCl, 1 mM EDTA, 150 mM NaCl, 1 mM DTT and 0.8 M ammonium sulfate pH 8.0 | Photinus pyralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-luciferin + O2 + ATP | - |
Photinus pyralis | oxyluciferin + CO2 + H2O + AMP + diphosphate + hv | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| firefly luciferase | - |
Photinus pyralis |
| Luc | - |
Photinus pyralis |
| Ppy | - |
Photinus pyralis |
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Release 2023.1 (January 2023)
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