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Literature summary for 1.13.12.7 extracted from

  • Mitani, Y.; Oshima, Y.; Mitsuda, N.; Tomioka, A.; Sukegawa, M.; Fujita, M.; Kaji, H.; Ohmiya, Y.
    Efficient production of glycosylated Cypridina luciferase using plant cells (2017), Protein Expr. Purif., 133, 102-109 .
    View publication on PubMed

Application

Application Comment Organism
analysis Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays Photinus pyralis
molecular biology Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays Photinus pyralis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression from vector p35SHSPG in Nicotiana benthamiana BY-2 cells with intracellular enzyme localization, recombinant expression of the enzyme in Arabidopsis thaliana roots with enzyme localization around the nucleus, outside of the central vacuole and in the protoplasm Photinus pyralis

Protein Variants

Protein Variants Comment Organism
additional information for large scale enzyme production, a simple production procedure is established using plant cell cultures. The plant cell culture tobacco BY-2 efficiently secretes luciferase, which is easily purified using a simple one-step ion-exchange chromatography method. The production yield is 20-30 mg of luciferase per liter of culture medium. The method offers a cost-effective production for Cypridina luciferase Photinus pyralis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Photinus pyralis 5737
-
nucleus associated to Photinus pyralis 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-firefly luciferin + O2 + ATP Photinus pyralis
-
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
?

Organism

Organism UniProt Comment Textmining
Photinus pyralis P08659
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted enzyme from Nicotiana benthamiana BY-2 cell medium by ultrafiltration, buffer exchange gel filtration, anion exchange chromatography, and dialysis Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-firefly luciferin + O2 + ATP
-
Photinus pyralis firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Photinus pyralis
fluc
-
Photinus pyralis