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Literature summary for 1.13.12.7 extracted from
- Random mutagenesis of Luciola mingrelica firefly luciferase. Mutant enzymes with bioluminescence spectra showing low pH sensitivity (2008), Biochemistry (Moscow), 73, 862-869.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 and XL1blue | Luciola mingrelica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F16L | lower pH-dependence of bioluminescence spectra, about 40% decrease in specific activity | Luciola mingrelica |
| F16L/A40S | bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 80% decrease in specific activity | Luciola mingrelica |
| S118C | increased thermostability, about 30% increase in specific activity | Luciola mingrelica |
| Y35H | bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 40% decrease in specific activity | Luciola mingrelica |
| Y35N | bioluminescence spectra independent from pH in range of pH 6.0-7.8, about 30% decrease in specific activity | Luciola mingrelica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Luciola mingrelica | Q26304 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Luciola mingrelica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| luciferin + ATP + O2 | - |
Luciola mingrelica | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| firefly luciferase | - |
Luciola mingrelica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
shift in bioluminescence spectra to red region by increase in temperature to 42°C | Luciola mingrelica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
50% decrease in activity after 6.9 min for wild-type luciferase, for mutant S118C after 13.4 min | Luciola mingrelica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at, shift in bioluminescence spectra to red region by increase in temperature to 42°C | Luciola mingrelica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.8 | bioluminescence spectra independent from pH, for mutants Y35N, Y35H and F16L/A40S | Luciola mingrelica |
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Release 2023.1 (January 2023)
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