Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Phrixothrix vivianii |
expressed in Escherichia coli XL1-Blue cells | Cratomorphus distinctus |
Protein Variants | Comment | Organism |
---|---|---|
F227Y | the mutation causes dramatic redshift and temporal shift | Cratomorphus distinctus |
F227Y | the mutation results in a unique time-dependent shift from red to yellow-green | Macrolampis sp2 |
F260L | the mutation results in a much less pH-sensitive enzyme displaying only a 10 nm redshift and broadening of the spectra at pH 6 | Cratomorphus distinctus |
G228A | the mutation results in a large redshift | Phrixothrix vivianii |
G228A | the mutation results in a large redshift | Macrolampis sp2 |
I226A | the mutation causes dramatic redshift and temporal shift | Cratomorphus distinctus |
L260F | mutation results in a 20 nm redshift without affecting pH-insensitivity | Pyrearinus termitilluminans |
V227A | mutant displays a small redshift | Pyrearinus termitilluminans |
V227F | mutation results in a 60 nm redshift | Pyrearinus termitilluminans |
V227I | mutant displays a small redshift | Pyrearinus termitilluminans |
V227S | mutant displays a small redshift | Pyrearinus termitilluminans |
Y227A | mutant shows enzymatic behaviour similar to the wild type enzyme | Phrixothrix hirtus |
Y227A | the mutation causes dramatic red- and time-dependent shifts | Phrixothrix vivianii |
Y227A | the substitution results in a weak and unstable red mutant | Macrolampis sp2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cratomorphus distinctus | Q5USC8 | - |
- |
Macrolampis sp2 | - |
- |
- |
Phrixothrix hirtus | Q9U4U7 | - |
- |
Phrixothrix vivianii | Q9U4U8 | - |
- |
Pyrearinus termitilluminans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
agarose-Ni2+ column chromatography | Phrixothrix vivianii |
agarose-Ni2+ column chromatography | Pyrearinus termitilluminans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + O2 + ATP | - |
Cratomorphus distinctus | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
D-luciferin + O2 + ATP | - |
Phrixothrix vivianii | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
D-luciferin + O2 + ATP | - |
Macrolampis sp2 | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
D-luciferin + O2 + ATP | - |
Pyrearinus termitilluminans | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
D-luciferin + O2 + ATP | - |
Phrixothrix hirtus | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beetle luciferase | - |
Cratomorphus distinctus |
beetle luciferase | - |
Phrixothrix vivianii |
beetle luciferase | - |
Macrolampis sp2 |
beetle luciferase | - |
Pyrearinus termitilluminans |
beetle luciferase | - |
Phrixothrix hirtus |
firefly luciferase | - |
Cratomorphus distinctus |
firefly luciferase | - |
Phrixothrix vivianii |
firefly luciferase | - |
Macrolampis sp2 |
firefly luciferase | - |
Pyrearinus termitilluminans |
firefly luciferase | - |
Phrixothrix hirtus |