Literature summary for 1.13.12.7 extracted from

Branchini, B.R.; Southworth, T.L.; Fontaine, D.M.; Davis, A.L.; Behney, C.E.; Murtiashaw, M.H.
A Photinus pyralis and Luciola italica chimeric firefly luciferase produces enhanced bioluminescence (2014), Biochemistry, 53, 6287-6289 .

Search for more literature for 1.13.12.7

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a chimeric enzyme PpyLit that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase, the recombinant chimeric enzyme PpyLit shows 1.8fold enhanced flash-height specific activity, 2.0fold enhanced integration-based specific activity, 2.9fold enhanced catalytic efficiency (kcat/Km), and a 1.4fold greater bioluminescence quantum yield compared to the wild-type Photinus pyralis luciferase. A chimeric enzyme with enhanced catalytic properties that are not simply the sum of the contributions of the two luciferases	Photinus pyralis
additional information	construction of a chimeric enzyme PpyLit that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase, the recombinant chimeric enzyme PpyLit shows 1.8fold enhanced flash-height specific activity, 2.0fold enhanced integration-based specific activity, 2.9fold enhanced catalytic efficiency (kcat/Km), and a 1.4fold greater bioluminescence quantum yield compared to the wild-type Photinus pyralis luciferase. A chimeric enzyme with enhanced catalytic properties that are not simply the sum of the contributions of the two luciferases	Luciola italica

Inhibitors

Inhibitors	Comment	Organism	Structure
dehydroluciferin	-	Photinus pyralis
L-AMP	a potent luciferase inhibitor	Photinus pyralis
oxyluciferin	-	Photinus pyralis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	Photinus pyralis	-	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?
D-firefly luciferin + O2 + ATP	Luciola italica	-	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Organism

Organism	UniProt	Comment	Textmining
Luciola italica	Q1AG35	-	-
Photinus pyralis	P08659	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	-	Photinus pyralis	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?
D-firefly luciferin + O2 + ATP	-	Luciola italica	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Synonyms

Synonyms	Comment	Organism
firefly luciferase	-	Photinus pyralis
Luciola italica luciferase	-	Luciola italica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Photinus pyralis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Photinus pyralis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Photinus pyralis
ATP	-	Luciola italica

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000038	-	L-AMP	pH 7.8, 25°C	Photinus pyralis
0.0005	-	oxyluciferin	pH 7.8, 25°C	Photinus pyralis
0.0005	-	dehydroluciferin	pH 7.8, 25°C	Photinus pyralis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)