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3.5.2.2: dihydropyrimidinase

This is an abbreviated version!
For detailed information about dihydropyrimidinase, go to the full flat file.

Word Map on EC 3.5.2.2

Reaction

5,6-dihydrouracil
+
H2O
=
3-ureidopropanoate

Synonyms

4,5-dihydropyrimidine amidohydrolase, aaHYD, bar9HYD, bpHYD, bsHYD, CRMP, D-CpHPG, D-HYD, D-hydantionase, D-hydantoin-hydrolyzing enzyme, D-hydantoinase, D-PfHYD, DHP, DHP-1, DHP-2, DHPase, dht, DHTase, dihydropyrimidase/hydantoinase, Dihydropyrimidinase, dihydropyrimidine amidohydrolase, dihydropyrimidine dehydrogenase, HDT, HYD, hydantoin peptidase, hydantoinase, hydropyrimidine hydrase, hyuH, L-Hyd, L-hydantoinase, microbial hydantoinase, P479, PA0441, PaDHPase, PYD2, PydB, pyrimidine hydrase, SmelDhp, thHYD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.2 dihydropyrimidinase

Cloned

Cloned on EC 3.5.2.2 - dihydropyrimidinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a series of mutants of the enzyme with the C-terminal residues either deleted or substituted are prepared, expression in Escherichia coli
-
cDNA sequences of dihydropyrimidinase determined
D-hydantoinase gene cloned, sequenced and expressed in Escherichia coli
-
D-hydantoinase genes cloned and sequenced, Escherichia coli transformed with cosmid vectors, D-hydantoinase expressed and identified
-
DL-hydantoinase cloned from 172-kb native plasmid
-
expressed by pET-HDT/Escherichia coli BL21
expressed in Escherichia coli
expressed in Escherichia coli B834
-
expressed in Escherichia coli BL21
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 cells in insoluble form
-
expressed in Escherichia coli strain DG116
-
expressed in Escherichia coli strain JM109, the expression level of dihydropyrimidinase in the recombinant Escherichia coli cells is estimated to be about 20% of the respective total soluble proteins
-
expressed in Escherichia coli strain pE-HDT/BL21
expressed in Escherichia coli strains strains BL21 DE3 and JM109 (as a His-tagged enzyme)
expression as His8-tagged protein in Escherichia coli BL21
-
expression in Escherichia coli
expression in Escherichia coli JM109
expression in Escherichia coli NovaBlue
-
expression in Escherichia coli, His-tag
functional recombinant expression in Escherichia coli strain BL21(DE3)
-
functional recombinant expression in Escherichia coli strain JM109
gene cloned and sequenced, identical with B. stearothermophilus NS1122A gene
-
gene for thermostable hydantoinase cloned and overexpressed in Escherichia coli JM105, vector pAHN134
-
gene hyd, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)-pET28-hyd/pGRO7, mostly in insoluble form. Five sets of molecular chaperones are coexpressed with D-PfHYD in Escherichia coli, including pGRO7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), pTF16 (Tigger), pGRO7-KJE8 (GroES-GroEL & DnaK-DnaJ-GrpE), and pGRO7-TF2, to improve the method. Coexpression of the chaperone GroES-GroEL is most efficient in improving the soluble expression of D-PfHYD
gene pydB, recombinant expression of poly-His tagged enzyme in Escherichia coli
overexpression as His8-tagged fusion protein in Escherichia coli BL21
-
overexpression of His6-tagged DHP in Escherichia coli Novablue cells
recombinant expression of His-tagged enzyme
recombinant expression of wild-type and mutant His-tagged enzymes
strain SD1, D-hydantoinase gene cloned and sequenced
-