3.5.2.2: dihydropyrimidinase
This is an abbreviated version!
For detailed information about dihydropyrimidinase, go to the full flat file.
Word Map on EC 3.5.2.2
-
3.5.2.2
-
5-fluorouracil
-
thymidylate
-
dihydropyridine
-
colorectal
-
fluoropyrimidine
-
phosphorylase
-
thymidine
-
capecitabine
-
l-type
-
uracil
-
nifedipine
-
orotate
-
resect
-
blocker
-
pharmacogenetic
-
1,4-dihydropyridine
-
tegafur
-
prodrugs
-
voltage-dependent
-
dihydrouracil
-
5-fu-based
-
fluoropyrimidine-based
-
nitrendipine
-
leucovorin
-
phosphoribosyltransferase
-
diltiazem
-
oxaliplatin
-
irinotecan
-
hemoperfusion
-
phosphoribosyl
-
5-fluorouracil-based
-
mucositis
-
neutropenia
-
pyrrolizidine
-
11-ketotestosterone
-
excitation-contraction
-
isradipine
-
nisoldipine
-
coniferyl
-
5-fu-induced
-
dihydropyridine-sensitive
-
felodipine
-
5\'-dfur
-
dihydroorotase
-
photochromic
-
nicardipine
-
synthesis
-
industry
-
5'-deoxy-5-fluorouridine
-
pretherapeutic
-
omega-conotoxin
-
nutrition
-
medicine
-
hand-foot
- 3.5.2.2
- 5-fluorouracil
- thymidylate
-
dihydropyridine
- colorectal
-
fluoropyrimidine
- phosphorylase
- thymidine
- capecitabine
-
l-type
- uracil
- nifedipine
- orotate
-
resect
-
blocker
-
pharmacogenetic
-
1,4-dihydropyridine
-
tegafur
-
prodrugs
-
voltage-dependent
- dihydrouracil
-
5-fu-based
-
fluoropyrimidine-based
-
nitrendipine
- leucovorin
-
phosphoribosyltransferase
- diltiazem
- oxaliplatin
- irinotecan
-
hemoperfusion
-
phosphoribosyl
-
5-fluorouracil-based
- mucositis
- neutropenia
-
pyrrolizidine
- 11-ketotestosterone
-
excitation-contraction
- isradipine
- nisoldipine
-
coniferyl
-
5-fu-induced
-
dihydropyridine-sensitive
- felodipine
-
5\'-dfur
- dihydroorotase
-
photochromic
- nicardipine
- synthesis
- industry
- 5'-deoxy-5-fluorouridine
-
pretherapeutic
-
omega-conotoxin
- nutrition
- medicine
-
hand-foot
Reaction
Synonyms
4,5-dihydropyrimidine amidohydrolase, aaHYD, bar9HYD, bpHYD, bsHYD, CRMP, D-CpHPG, D-HYD, D-hydantionase, D-hydantoin-hydrolyzing enzyme, D-hydantoinase, D-PfHYD, DHP, DHP-1, DHP-2, DHPase, dht, DHTase, dihydropyrimidase/hydantoinase, Dihydropyrimidinase, dihydropyrimidine amidohydrolase, dihydropyrimidine dehydrogenase, HDT, HYD, hydantoin peptidase, hydantoinase, hydropyrimidine hydrase, hyuH, L-Hyd, L-hydantoinase, microbial hydantoinase, P479, PA0441, PaDHPase, PYD2, PydB, pyrimidine hydrase, SmelDhp, thHYD
ECTree
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Substrates Products
Substrates Products on EC 3.5.2.2 - dihydropyrimidinase
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REACTION DIAGRAM
5-(2-thienyl)-DL-hydantoin + H2O
N-carbamoyl-D-2-thienylglycine
-
-
-
-
?
5-(4-hydroxyphenyl)hydantoin + H2O
5-(4-hydroxyphenyl)-D-carbamoylate
-
-
-
-
?
D-2-phenylhydantoic acid + H2O
5-phenylhydantoin
-
reverse reaction stereospecific, only D-isomer acts as substrate
-
-
r
D-5-(1-methylpropyl)hydantoin + H2O
N-carbamoyl-D-isoleucine
-
-
-
?
dimethylhydantoin + H2O
?
1.4% of the activity with dihydrouracil
-
-
?
DL-4-hydroxyphenylhydantoin + H2O
D-carbamoyl-4-hydroxyphenylglycine
-
-
-
-
?
DL-5-(2-carboxyethyl)hydantoin + H2O
L-glutamic acid
-
-
exclusively L-form
?
DL-methylthioethylhydantoin + H2O
?
-
D-selective for the hydrolysis
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-p-hydroxyphenylglycine
7.2% of the activity with dihydrouracil
-
-
?
L-5-(1-methylpropyl)hydantoin + H2O
N-carbamoyl-L-isoleucine
-
-
-
?
L-5-benzylhydantoin + H2O
N-carbamoyl-L-phenylalanine
-
-
-
?
L-5-methylhydantoin + H2O
N-carbamoyl-L-alanine
-
-
-
?
N-carbamoyl-L-tryptophan + H2O
L-tryptophan + ?
-
N-carbamoyl-D-tryptophan is no substrate
-
-
?
rac-5-p-fluorophenylhydantoin + H2O
?
-
immobilized enzyme (linked to aminopropyl glass beads) converts 100%
-
-
?
rac-5-p-trifluoromethylphenylhydantoin + H2O
?
-
immobilized enzyme (linked to aminopropyl glass beads) converts 87%
-
-
?
rac-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
immobilized enzyme (linked to aminopropyl glass beads) converts 100%
-
-
?
?
NCarbpNO2betaPhe, chemically synthesized artificial substrate. Enantioselective reactivity, preference of the hydantoinase for the (S)-enantiomer
-
-
?
3-(4-nitrophenyl)-3-ureidopropanoic acid + H2O
?
NCarbpNO2betaPhe, chemically synthesized artificial substrate. Enantioselective reactivity, preference of the hydantoinase for the (S)-enantiomer
-
-
?
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
?
4-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
Pseudomonas fluorescens CGMCC 1.1802
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
-
-
-
-
r
5,6-dihydropyrimidine + H2O
beta-ureido acid
-
-
-
-
?
3-(carbamoylamino)-2-methylpropanoic acid
-
-
-
?
5,6-dihydrothymine + H2O
3-(carbamoylamino)-2-methylpropanoic acid
-
-
-
?
3-ureidopropanoate
-
26% relative enzyme activity
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
26% relative enzyme activity
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropanoate
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
purified preparation demonstrates no activity on this substrate
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
purified preparation demonstrates no activity on this substrate
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
172058, 209278, 209282, 209283, 209284, 209286, 209287, 209293, 209294, 209298, 209299, 209301, 685395
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
209273, 209274, 209275, 209279, 209280, 209281, 209283, 209289, 209290, 209293, 209294, 209298, 209299, 209301, 209303, 687942
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
-
-
-
-
?
?
highest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
5,6-dihydrouracil + H2O
?
highest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
5,6-dihydrouracil + H2O
?
2.35fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
5,6-dihydrouracil + H2O
?
2.35fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
N-carbamoyl-D-4-hydroxyphenylglycine
-
-
-
?
5-(4-hydroxyphenyl) hydantoin + H2O
N-carbamoyl-D-4-hydroxyphenylglycine
-
-
-
?
2-fluoro-3-ureidopropionic acid
growth of GMP1 and GMP2 mutants but not of wild-type RU-KM3S, mutants independent of inducer
-
-
?
5-fluorouracil + H2O
2-fluoro-3-ureidopropionic acid
growth of GMP1 and GMP2 mutants but not of wild-type RU-KM3S, mutants independent of inducer
-
-
?
2-fluoro-3-ureidopropionic acid + ?
growth of GMP1 and GMP2 mutants and wild-type RU-KM3S
-
-
?
5-fluorouracil + hydantoin
2-fluoro-3-ureidopropionic acid + ?
growth of GMP1 and GMP2 mutants and wild-type RU-KM3S
-
-
?
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
5-propylhydantoin + H2O
N-carbamoyl-D-valine
low activity
-
-
?
?
pNO2PheDU, chemically synthesized artificial substrate. Recombinant Escherichia coli cells expressing the hydantoinase show degradation of the substrate. Enantioselective reactivity, preference of the hydantoinase for the (S)-enantiomer
-
-
?
6-(4-nitrophenyl)dihydropyrimidine-2,4(1H,3H)-dione + H2O
?
pNO2PheDU, chemically synthesized artificial substrate. Recombinant Escherichia coli cells expressing the hydantoinase show degradation of the substrate. Enantioselective reactivity, preference of the hydantoinase for the (S)-enantiomer
-
-
?
N-carbamoyl-D-methionine
-
-
-
-
?
D-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
?
D-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
N-carbamoyl-D-phenylalanine
-
-
-
?
N-carbamoyl-D-leucine
-
-
-
?
D-5-isobutylhydantoin + H2O
N-carbamoyl-D-leucine
-
-
-
?
N-carbamoyl-D-valine
-
-
-
?
D-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
-
L-5-methylhydantoin is no substrate
-
-
?
?
1.57fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
D-methylhydantoin + H2O
?
1.57fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
?
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
DL-5(2-(methylthio)-ethyl)hydantoin + H2O
?
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
strictly D-selective
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
Bacillus sp. (in: Bacteria) LU 1220
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
the enzyme exhibits strict D-enantioselectivity for DL-5-(2-methylthioethyl)-hydantoin
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
the enzyme exhibits strict D-enantioselectivity for DL-5-(2-methylthioethyl)-hydantoin
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
steric configuration D
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
Streptomyces mitakaensis
-
-
-
-
?
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
produces stereospecifically D-p-hydroxyphenylglycine
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
Bacillus sp. (in: Bacteria) LU 1220
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
Pseudomonas putida CCRC 12857 / ATCC 950
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
-
-
-
-
?
L-tryptophan
-
strictly D-selective
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
-
strictly L-selective for the cleavage
-
-
?
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
only D-isomer hydrolyzed
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
only D-isomer hydrolyzed
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
-
-
-
?
D-p-hydroxyphenylglycine
-
hydantoinase and carbamylase involved
-
-
?
DL-5-p-hydroxyphenylhydantoin + H2O
D-p-hydroxyphenylglycine
-
hydantoinase and carbamylase involved
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
45% of the activity with dihydrouracil
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
r
4-chloro-N-carbamoyl-beta-phenylalanine
-
-
-
-
?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
4-chloro-N-carbamoyl-beta-phenylalanine
-
-
-
-
?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
4-chloro-N-carbamoyl-beta-phenylalanine
-
-
-
-
?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
4-chloro-N-carbamoyl-beta-phenylalanine
Delftia sp. I24 DSM 18833
-
-
-
-
?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
4-chloro-N-carbamoyl-beta-phenylalanine
-
-
-
-
?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
4-chloro-N-carbamoyl-beta-phenylalanine
Ochrobactrum sp. G21 DSM 18828
-
-
-
-
?
N-rac-carbamoyl-beta-phenylalanine
-
-
27% conversion. Enzyme preferably hydrolyzes L-Phe-5,6-dihydrouracil to L-N-carbamoyl-beta-phenylalanine, 61% enantiomeric excess of L-enantiomer
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
-
-
27% conversion. Enzyme preferably hydrolyzes L-Phe-5,6-dihydrouracil to L-N-carbamoyl-beta-phenylalanine, 61% enantiomeric excess of L-enantiomer
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
-
-
enzyme does not display a significant entantioselectivity
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
Delftia sp. I24 DSM 18833
-
-
enzyme does not display a significant entantioselectivity
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
-
-
enzyme does not display a significant entantioselectivity
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
Ochrobactrum sp. G21 DSM 18828
-
-
enzyme does not display a significant entantioselectivity
-
?
?
-
the enzyme exhibits non-enantiospecificity for DL-homophenylalanylhydantoin
-
-
?
DL-homophenylalanylhydantoin + H2O
?
-
the enzyme exhibits non-enantiospecificity for DL-homophenylalanylhydantoin
-
-
?
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
-
?
?
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
?
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
N-carbamoyl-D-hydroxyphenylglycine
-
19% relative enzyme activity
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
19% relative enzyme activity
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
the enzyme exhibits strict D-enantioselectivity for DL-p-hydroxyphenylhydantoin
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
the enzyme exhibits strict D-enantioselectivity for DL-p-hydroxyphenylhydantoin
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
-
-
-
?
N-carbamoyl-D-phenylglycine
-
39.9% relative enzyme activity
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
39.9% relative enzyme activity
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
-
-
-
-
?
?
lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
hydantoin + H2O
?
lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
hydantoin + H2O
hydantoic acid
-
-
-
-
?
hydantoin + H2O
hydantoic acid
-
-
-
-
?
hydantoin + H2O
hydantoic acid
18.7% of the activity with dihydrouracil
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
preferred substrate, 100% relative enzyme activity
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
preferred substrate, 100% relative enzyme activity
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
172058, 209272, 209276, 209278, 209282, 209283, 209284, 209287, 209291, 209293, 209294, 209298, 209299, 209301
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
209273, 209274, 209275, 209279, 209280, 209281, 209283, 209289, 209290, 209293, 209294, 209297, 209298, 209299, 209301, 209302, 209303, 684522
-
-
?
hydantoin + H2O
N-carbamoyl glycine
4fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
hydantoin + H2O
N-carbamoyl glycine
4fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
-
-
-
-
?
N-carbamoyl-D-alpha-isoleucine
-
-
-
?
isobutylhydantoin + H2O
N-carbamoyl-D-alpha-isoleucine
98.7 % enantiomeric excess (ee), isolation yield of 78.3%, and substrate to biocatalyst ratio of 15.6 in recombinant Escherichia coli
-
-
?
isobutylhydantoin + H2O
N-carbamoyl-D-alpha-isoleucine
Pseudomonas fluorescens CGMCC 1.1802
98.7 % enantiomeric excess (ee), isolation yield of 78.3%, and substrate to biocatalyst ratio of 15.6 in recombinant Escherichia coli
-
-
?
N-carbamoyl-L-methionine
-
-
-
?
L-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-L-methionine
-
-
-
?
N-carbamoyl-L-leucine
-
-
-
?
L-5-isobutylhydantoin + H2O
N-carbamoyl-L-leucine
-
-
-
?
additional information
?
-
-
disubstituted hydantoins or 1-substituted hydantoins are no substrates
-
-
?
additional information
?
-
-
disubstituted hydantoins or 1-substituted hydantoins are no substrates
-
-
?
additional information
?
-
the enzyme shows activity with 6-monosubstituted dihydrouracils. The enzyme shows enantioselectivity
-
-
-
additional information
?
-
the enzyme shows activity with 6-monosubstituted dihydrouracils. The enzyme shows enantioselectivity
-
-
-
additional information
?
-
-
DL-hydroxymethylhydantoin and 5,5-disubstituted hydantoin derivatives are no substrates
-
-
?
additional information
?
-
-
DL-hydroxymethylhydantoin and 5,5-disubstituted hydantoin derivatives are no substrates
-
-
?
additional information
?
-
-
inactive with carbamoyl beta-alanine amide, barbituric acid, imidazole 4,5-dicarboxylic acid, 5-(carboxymethylidine)hydantoin, orotic acid, 4,5-aminoimidazolecarboximide, urocanic acid and 5-(diphenyl)hydantoin
-
-
?
additional information
?
-
the Brevibacillus agri strain NCHU1002 can perform the ring opening reaction of D,L-5-monosubstituted hydantoins stereoselectively to N-carbamoyl-D-amino acids. No L-form amino acid are formed in the reaction, analysis via the chiral HPLC
-
-
?
additional information
?
-
the Brevibacillus agri strain NCHU1002 can perform the ring opening reaction of D,L-5-monosubstituted hydantoins stereoselectively to N-carbamoyl-D-amino acids. No L-form amino acid are formed in the reaction, analysis via the chiral HPLC
-
-
?
additional information
?
-
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
-
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
no substrate: diphenylhydantoin, 5-(hydroxymethyl)uracil, benzylhydantoin, isopropylhydantoin
-
-
?
additional information
?
-
-
DL-5-phenylhydantoin is no substrate
-
-
?
additional information
?
-
-
hydantoin, urea, allantoin and dihydroorotate are no substrates
-
-
?
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-
additional information
?
-
the catalytic efficiency of the enzyme toward dihydrouracil is higher than that toward phthalimide (fourfold) and 5-propylhydantoin (100fold). Therefore, this bacterial enzyme is suitably identified as a dihydropyrimidinase, not a hydantoinase. Allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
purified D-PfHYD prefers aliphatic to aromatic 5'-monosubstituted hydantoins. 5'-Monosubstituted hydantoins give N-carbamoyl-D-alpha-amino acids
-
-
-
additional information
?
-
-
purified D-PfHYD prefers aliphatic to aromatic 5'-monosubstituted hydantoins. 5'-Monosubstituted hydantoins give N-carbamoyl-D-alpha-amino acids
-
-
-
additional information
?
-
Pseudomonas fluorescens CGMCC 1.1802
purified D-PfHYD prefers aliphatic to aromatic 5'-monosubstituted hydantoins. 5'-Monosubstituted hydantoins give N-carbamoyl-D-alpha-amino acids
-
-
-
additional information
?
-
-
hydantoins derived from alanine, valine, leucine, serine, phenylalanine and hydroxyphenylglycine are substrates, hydantoins derived from aspartic acid, lysine, and 5,5-disubstituted hydantoins are not hydrolyzed
-
-
?
additional information
?
-
no activity towards uracil and succinimide
-
-
?
additional information
?
-
-
catalyzes the hydrolysis of 5-monosubstituted hydantoin to enantionmeric N-carbamoyl-amino acids
-
-
?
additional information
?
-
-
catalyzes the hydrolysis of 5-monosubstituted hydantoin to enantionmeric N-carbamoyl-amino acids
-
-
?
additional information
?
-
no activity towards uracil and succinimide
-
-
?
additional information
?
-
-
diphenylhydantoin and 5,5-disubstituted hydantoins like 5-ethyl-5-phenylhydantoin, 5,5-diphenylhydantoin, D-3-methyl-5-phenylhydantoin, D-3-ethyl-5-phenylhydantoin, alpha-methyl-alpha-phenylsuccinimide, N-methyl-alpha-phenylsuccinimide and alpha-ethyl-alpha-phenylglutarimide are no substrates
-
-
?
additional information
?
-
-
succinimide, dihydro-L-orotic-2-acid, dihydrouridine and dihydrothymidine are no substrates
-
-
?
additional information
?
-
-
diphenylhydantoin and 5,5-disubstituted hydantoins like 5-ethyl-5-phenylhydantoin, 5,5-diphenylhydantoin, D-3-methyl-5-phenylhydantoin, D-3-ethyl-5-phenylhydantoin, alpha-methyl-alpha-phenylsuccinimide, N-methyl-alpha-phenylsuccinimide and alpha-ethyl-alpha-phenylglutarimide are no substrates
-
-
?
additional information
?
-
-
enzyme presents both hydantoinase and dihydropyrimidinase activities, with higher affinity for the natural six-membered ring substrates. For the five-membered ring substrates, affinity is greater for those with aliphatic and apolar groups in the 5th carbon atom, with the highest rates of hydrolysis for D-5-methyl and D-5-ethyl hydantoin. Enzyme is enantioselective for the D-isomer of monosubstituted 5-hydantoins. Activity with substrate analogues such as allantoin, 5,5-dimethylhydantoin, 5-benzyl,5-methyl-hydantoin, succinimide, rodamine or tiazoledione is not detected under the conditions assayed
-
-
?
additional information
?
-
-
one zinc ion is sufficient to stabilize Lys carbamylation in Tetraodon nigroviridis dihydropyrimidinase (DHPase)
-
-
-