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3.5.2.2: dihydropyrimidinase

This is an abbreviated version!
For detailed information about dihydropyrimidinase, go to the full flat file.

Word Map on EC 3.5.2.2

Reaction

5,6-dihydrouracil
+
H2O
=
3-ureidopropanoate

Synonyms

4,5-dihydropyrimidine amidohydrolase, aaHYD, bar9HYD, bpHYD, bsHYD, CRMP, D-CpHPG, D-HYD, D-hydantionase, D-hydantoin-hydrolyzing enzyme, D-hydantoinase, D-PfHYD, DHP, DHP-1, DHP-2, DHPase, dht, DHTase, dihydropyrimidase/hydantoinase, Dihydropyrimidinase, dihydropyrimidine amidohydrolase, dihydropyrimidine dehydrogenase, HDT, HYD, hydantoin peptidase, hydantoinase, hydropyrimidine hydrase, hyuH, L-Hyd, L-hydantoinase, microbial hydantoinase, P479, PA0441, PaDHPase, PYD2, PydB, pyrimidine hydrase, SmelDhp, thHYD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.2 dihydropyrimidinase

Inhibitors

Inhibitors on EC 3.5.2.2 - dihydropyrimidinase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
at 1 mM 47% and at 2 mM 46% residual enzyme acitvity
2,2'-dipyridyl
2,6-dipicolinic acid
3-Amino-1,2,4-triazole
-
4-nitrobenzene sulfonamide
-
-
4-ureidobutyric acid
-
-
5-amino-n-valeric acid
-
-
5-hydantoin acetic acid
-
8-hydroxyquinoline
8-Hydroxyquinoline-5-sulfonic acid
Acetohydroxamate
-
allantoin
slight inhibition
beta-Alanine
-
-
Ca2+
97% residual activity at 1 mM
Cd2+
44% residual activity at 1 mM
chloramphenicol
-
10.5fold reduced activity in chloramphenicol-treated cells
D-glucose
-
-
diethylpyrocarbonate
-
at 2 mM 95% and at 5 mM 80% residual enzyme acitvity
dihydromyricetin
competitive inhibitor, dihydromyricetin is docked in the active site pocket of dihydropyrimidinase, binding kinetics. Dihydromyricetin forms a stable complex with dihydropyrimidinase with the Kd value of 0.0226 mM
dihydroorotate
slight inhibition
dimethylhydantoin
-
competitive inhibition
glutaric acid monoamide
-
-
L-dihydroorotic acid
-
competitive inhibition
Mercuric chloride
-
1 mM completely abrogates activity
Mg2+
-
slightly inhibitory with carbamoyl beta-alanine or carbamoyl beta-aminoisobutyric acid as substrates
N-bromosuccinimide
-
1 mM completely abrogates activity
N-Carbamoyl-beta-alanine
N-carbamoyl-beta-aminoisobutyric acid
-
product inhibition
N-Carbamoyl-glycine
-
competitive inhibition
nitrilotriacetic acid
-
inhibition 42%
o-phenanthroline
p-hydroxymercuribenzoate
p-hydroxymercuric benzoate
-
1 mM completely abrogates activity
propionic acid
-
-
propionic acid amide
-
-
rac-5-methyl-5-phenylhydantoic acid
-
competitive
Sn2+
-
-
trans-1,2-cyclohexandiaminetetraacetic acid
-
inhibition 42%
additional information
-