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3.5.2.2: dihydropyrimidinase

This is an abbreviated version!
For detailed information about dihydropyrimidinase, go to the full flat file.

Word Map on EC 3.5.2.2

Reaction

5,6-dihydrouracil
+
H2O
=
3-ureidopropanoate

Synonyms

4,5-dihydropyrimidine amidohydrolase, aaHYD, bar9HYD, bpHYD, bsHYD, CRMP, D-CpHPG, D-HYD, D-hydantionase, D-hydantoin-hydrolyzing enzyme, D-hydantoinase, D-PfHYD, DHP, DHP-1, DHP-2, DHPase, dht, DHTase, dihydropyrimidase/hydantoinase, Dihydropyrimidinase, dihydropyrimidine amidohydrolase, dihydropyrimidine dehydrogenase, HDT, HYD, hydantoin peptidase, hydantoinase, hydropyrimidine hydrase, hyuH, L-Hyd, L-hydantoinase, microbial hydantoinase, P479, PA0441, PaDHPase, PYD2, PydB, pyrimidine hydrase, SmelDhp, thHYD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.2 dihydropyrimidinase

Specific Activity

Specific Activity on EC 3.5.2.2 - dihydropyrimidinase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.005
-
strain 30207, isopropylhydantoin as substrate
0.00569
-
at 30°C
0.006
-
strain 30201, isopropylhydantoin as substrate
0.007
-
strain 30205, isopropylhydantoin as substrate
0.009
-
strain 30199, isopropylhydantoin as substrate
0.01
-
strain 50106, isopropylhydantoin as substrate
0.011
-
strain 2005, isopropylhydantoin as substrate
0.013
-
strain 30150, isopropylhydantoin as substrate
0.014
0.015
-
strain 50090, incubated with hydantoin, isopropylhydantoin as substrate
0.016
0.019
-
strain 50154, isopropylhydantoin as substrate
0.021
-
strain 30204, isopropylhydantoin as substrate
0.022
-
strain 2005, isopropylhydantoin as substrate
0.023
-
strain 30147, isopropylhydantoin as substrate
0.033
0.034
-
strain 291, isopropylhydantoin as substrate
0.038
-
strain 84, isopropylhydantoin as substrate
0.049
-
strain 549, isopropylhydantoin as substrate
0.051
-
strain 291, incubated with hydantoin, isopropylhydantoin as substrate
0.059
-
strain 11291, dihydrouracil as substrate
0.07
-
substrate D-benzylhydantoin
0.084
-
DL-5-p-hydroxyphenylhydantoin conversion
0.085
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-(1-methylpropyl)hydantoin
0.091
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-hydantoin-5-acetic acid
0.094
-
strain 11291, incubated with hydantoin
0.1
-
10 mM dihydrouracil as substrate
0.112
-
strain 30205, dihydrouracil as substrate
0.127
-
strain 549, incubated with hydantoin, isopropylhydantoin as substrate
0.128
-
strain 30150, dihydrouracil as substrate
0.15
-
strain 2005, dihydrouracil as substrate
0.17
EDTA-dialyzed/Co2+-substituted enzyme incubated with hydantoin as substrate
0.185
-
strain 84, incubated with hydantoin, isopropylhydantoin as substrate
0.19
-
strain 50154, dihydrouracil as substrate
0.193
-
strain 30204, dihydrouracil as substrate
0.194
-
strain 50090, dihydrouracil as substrate
0.212
-
strain 30147, dihydrouracil as substrate
0.216
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-methylhydantoin
0.225
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-hydroxymethylhydantoin
0.299
-
strain 30155, dihydrouracil as substrate
0.31
-
strain 50106, dihydrouracil as substrate
0.317
-
strain 30208, dihydrouracil as substrate
0.36
EDTA-dialyzed enzyme incubated with Fe2+ with DL-p-hydroxyphenylhydantoin as substrate
0.37
-
substrate L-benzylhydantoin
0.377
-
DL-5-p-hydroxyphenylhydantoin conversion induced by hydantoin
0.385
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-p-hydroxyphenylhydantoin
0.4
-
10 mM dihydrothymine as substrate
0.425
-
strain 30207, dihydrouracil as substrate
0.46
EDTA-dialyzed enzyme incubated with Ca2+ with DL-p-hydroxyphenylhydantoin as substrate
0.49
EDTA-dialyzed enzyme incubated with Ni2+ with DL-p-hydroxyphenylhydantoin as substrate
0.5
-
strain 291, dihydrouracil as substrate
0.52
EDTA-dialyzed enzyme with D,L-p-hydroxyphenylhydantoin as substrate
0.53
-
strain 50108, dihydrouracil as substrate
0.54
EDTA-dialyzed enzyme incubated with Mg2+ with DL-p-hydroxyphenylhydantoin as substrate
0.562
-
strain 30206, dihydrouracil as substrate
0.6
-
DL-methylhydantoin, 57 mM
0.635
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-phenylhydantoin
0.68
EDTA-dialyzed enzyme incubated with Cu2+ with DL-p-hydroxyphenylhydantoin as substrate
0.74
substrate 5,6-dihydrouracil, pH 8.0, 25°C, purified recombinant enzyme in presence of 1 mM Cd2+
0.78
-
dihydrouracil as substrate
0.81
-
strain 50154, incubated with hydantoin, dihydrouracil as substrate
0.826
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-phenylpropylhydantoin
0.89
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-methylthiethylhydantoin
0.9
crude extract
0.91
substrate 5,6-dihydrouracil, pH 8.0, 25°C, purified recombinant enzyme in presence of 1 mM Ni2+
0.98
-
strain 50108, incubated with hydantoin, dihydrouracil as substrate
1.06
-
-
1.11
-
strain 50106, incubated with hydantoin, dihydrouracil as substrate
1.128
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-isobutylhydantoin
1.14
-
strain 30155, dihydrouracil as substrate
1.2
-
strain 549, dihydrouracil as substrate
1.286
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-benylhydantoin
1.4
-
strain 50090, incubated with hydantoin, dihydrouracil as substrate
1.44
-
DL-hydantoin as substrate
1.5
-
L-methylthioethylhydantoin, 2 mM
1.591
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-(2-indolymethyl)hydantoin
1.67
-
DL-5-methylhydantoin as substrate
1.69
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-p-hydroxyphenylhydantoin as substrate
1.72
substrate 5,6-dihydrouracil, pH 8.0, 25°C, purified recombinant enzyme in presence of 1 mM Mn2+
1.9
-
strain 549, incubated with hydantoin, dihydrouracil as substrate
10.1
-
-
10.4
-
-
10.65
after 3.4fold purification, using DL-5-phenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
10.75
10.9
-
isopropylhydantoin as substrate
101.5
-
hydantoin as substrate
103.6
-
after 334fold purification, at 25°C and pH 7.5
11.84
after 3.4fold purification, using DL-p-hydroxyphenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
12
-
L-methylthioethylhydantoin, 20 mM
13
-
DL-5-indolylmethylhydantoin as substrate
13 - 18
-
-
136.8
-
-
14.3
crude extract, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
2.25
-
incubated with hydantoin
2.38
EDTA-dialyzed enzyme incubated with Mn2+ with DL-p-hydroxyphenylhydantoin as substrate
2.9
substrate 5,6-dihydrouracil, pH 8.0, 25°C, purified recombinant enzyme in presence of 1 mM Zn2+
20.9
purified recombinant enzyme, pH 9.0, 45°C
216
-
DL-5-phenylhydantoin as substrate
236.9
-
phenylhydantoin as substrate
27.5
-
dihydrouracil as substrate
3.1
after 3.4fold purification
3.56
EDTA-dialyzed/Co2+-substituted enzyme incubated with dihydrouracil as substrate
3.6
EDTA-dialyzed enzyme incubated with Co2+ with DL-p-hydroxyphenylhydantoin as substrate
30
-
DL-methylthioethylhydantoin, 40 mM
30.3
-
crystals
37
-
D-methylthioethylhydantoin, 20 mM
48.5
after 3.4fold purification, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
5.34
crude enzyme, pH 9.0, 45°C, substrate isobutylhydantoin
5.4
-
D-methylthioethylhydantoin, 2 mM
5.79
purified recombinant His-tagged enzyme, pH 8.0, 50°C
5.8 - 5.9
substrate 5,6-dihydrouracil, pH 8.0, 25°C, purified recombinant enzyme in presence of 1 mM Co2+
5.93
purified recombinant enzyme from Escherichia coli JM109pJAVI2
6.6
-
mutant P477, free enzyme
600.8
after 3.4fold purification, using 5,6-dihydrouracil as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
613
-
mutant P475, free enzyme
66.2
-
-
70
-
L-5-indolylmethylhydantoin as substrate
76.6
-
DL-fluorobromylhydantoin as substrate
8.1
-
mutant P476, free enzyme
8.2
40°C, pH 8.0
9.32
-
mutant P478, ifree enzyme
91.6
-
hydroxyphenylhydantoin as substrate
additional information