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Literature summary for 3.5.2.2 extracted from

  • Seung-Goo, L.; Dong-Cheol, L.; Hak-Sung, K.
    Purification and characterization of thermostable D-hydantoinase from thermophilic Bacillus stearothermophilus SD-1 (1997), Appl. Biochem. Biotechnol., 62, 251-266.
    View publication on PubMed

Application

Application Comment Organism
synthesis
-
Thermus sp.
synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
-
Geobacillus stearothermophilus
D-hydantoinase genes cloned and sequenced, Escherichia coli transformed with cosmid vectors, D-hydantoinase expressed and identified Thermus sp.
expressed in Escherichia coli BL21 Thermus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Geobacillus stearothermophilus
Cu2+
-
Pseudomonas fluorescens
N-Carbamoyl-glycine competitive inhibition Geobacillus stearothermophilus
Zn2+
-
Geobacillus stearothermophilus
Zn2+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
dihydrouracil
-
Geobacillus stearothermophilus
32.1
-
phenylhydantoin
-
Geobacillus stearothermophilus
47.2
-
p-hydroxyphenylhydantoin
-
Geobacillus stearothermophilus
340
-
hydantoin
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Bos taurus
Mn2+
-
Thermus sp.
Mn2+ requires 1 ion per molecule of enzyme for activity Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51050
-
determined by mass spectrometer Geobacillus stearothermophilus
51100
-
2 * 51100, mass spectrometric analysis Geobacillus stearothermophilus
54000
-
2 * 54000, SDS-PAGE Geobacillus stearothermophilus
102100
-
mass spectrometric analysis Geobacillus stearothermophilus
133900
-
gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6-dihydrouracil + H2O Bos taurus
-
3-ureidopropionate
-
?
5,6-dihydrouracil + H2O Geobacillus stearothermophilus
-
3-ureidopropionate
-
?
5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
3-ureidopropionate
-
?
5,6-dihydrouracil + H2O Pseudomonas putida
-
3-ureidopropionate
-
?
5,6-dihydrouracil + H2O Thermus sp.
-
3-ureidopropionate
-
?
5,6-dihydrouracil + H2O Geobacillus stearothermophilus SD-1
-
3-ureidopropionate
-
?
hydantoin + H2O Rattus norvegicus
-
N-carbamoyl glycine
-
?
hydantoin + H2O Bos taurus
-
N-carbamoyl glycine
-
?
hydantoin + H2O Geobacillus stearothermophilus
-
N-carbamoyl glycine
-
?
hydantoin + H2O Pseudomonas fluorescens
-
N-carbamoyl glycine
-
?
hydantoin + H2O Pseudomonas putida
-
N-carbamoyl glycine
-
?
hydantoin + H2O Thermus sp.
-
N-carbamoyl glycine
-
?
hydantoin + H2O Agrobacterium sp.
-
N-carbamoyl glycine
-
?
hydantoin + H2O Geobacillus stearothermophilus SD-1
-
N-carbamoyl glycine
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium sp.
-
-
-
Bos taurus
-
calf
-
Bos taurus
-
bovine
-
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus SD-1
-
-
-
Pseudomonas fluorescens
-
-
-
Pseudomonas putida
-
-
-
Rattus norvegicus
-
-
-
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus
-
Pseudomonas fluorescens
-
Pseudomonas putida
-
Agrobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
148.6
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + H2O
-
Rattus norvegicus 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Bos taurus 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Geobacillus stearothermophilus 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Pseudomonas fluorescens 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Pseudomonas putida 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Thermus sp. 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Agrobacterium sp. 3-ureidopropionate
-
?
5,6-dihydrouracil + H2O
-
Geobacillus stearothermophilus SD-1 3-ureidopropionate
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
Geobacillus stearothermophilus N-carbamoyl-p-hydroxyphenylglycine
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
Pseudomonas putida N-carbamoyl-p-hydroxyphenylglycine
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
Agrobacterium sp. N-carbamoyl-p-hydroxyphenylglycine
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
Geobacillus stearothermophilus SD-1 N-carbamoyl-p-hydroxyphenylglycine
-
?
DL-5-phenylhydantoin + H2O
-
Rattus norvegicus N-carbamoyl-D-phenylglycine
-
?
DL-5-phenylhydantoin + H2O
-
Bos taurus N-carbamoyl-D-phenylglycine
-
?
DL-5-phenylhydantoin + H2O
-
Geobacillus stearothermophilus N-carbamoyl-D-phenylglycine
-
?
DL-5-phenylhydantoin + H2O
-
Pseudomonas putida N-carbamoyl-D-phenylglycine
-
?
DL-5-phenylhydantoin + H2O
-
Geobacillus stearothermophilus SD-1 N-carbamoyl-D-phenylglycine
-
?
hydantoin + H2O
-
Bos taurus hydantoic acid
-
?
hydantoin + H2O
-
Geobacillus stearothermophilus hydantoic acid
-
?
hydantoin + H2O
-
Pseudomonas fluorescens hydantoic acid
-
?
hydantoin + H2O
-
Thermus sp. hydantoic acid
-
?
hydantoin + H2O
-
Geobacillus stearothermophilus SD-1 hydantoic acid
-
?
hydantoin + H2O
-
Rattus norvegicus N-carbamoyl glycine
-
?
hydantoin + H2O
-
Bos taurus N-carbamoyl glycine
-
?
hydantoin + H2O
-
Geobacillus stearothermophilus N-carbamoyl glycine
-
?
hydantoin + H2O
-
Pseudomonas fluorescens N-carbamoyl glycine
-
?
hydantoin + H2O
-
Pseudomonas putida N-carbamoyl glycine
-
?
hydantoin + H2O
-
Thermus sp. N-carbamoyl glycine
-
?
hydantoin + H2O
-
Agrobacterium sp. N-carbamoyl glycine
-
?
hydantoin + H2O
-
Geobacillus stearothermophilus SD-1 N-carbamoyl glycine
-
?

Subunits

Subunits Comment Organism
dimer
-
Thermus sp.
dimer 2 * 54000, SDS-PAGE Geobacillus stearothermophilus
dimer 2 * 51100, mass spectrometric analysis Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
hydantoin as substrate Geobacillus stearothermophilus
70
-
-
Agrobacterium sp.
70
-
DL-p-hydroxyphenylhydantoin as substrate Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
-
Pseudomonas putida
60
-
stable up to Geobacillus stearothermophilus
70
-
treatment for 30 min, 20% activity remains Agrobacterium sp.
80
-
half-life 30 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus stearothermophilus
8
-
-
Pseudomonas fluorescens
8.8
-
-
Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
remains stable at pH values higher than Geobacillus stearothermophilus