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Literature summary for 3.5.2.2 extracted from

  • Clemente-Jimenez, J.M.; Martinez-Rogriguez, S.; Mingorance-Cazorla, L.; de la Escalera-Hueso, S.; las Heras-Vazquez, F.J.; Rodriguez-Vico, F.
    Catalytic analysis of a recombinant D-hydantoinase from Agrobacterium tumefaciens (2003), Biotechnol. Lett., 25, 1067-1073.
    View publication on PubMed
Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 70 mM Agrobacterium tumefaciens
Hg2+
-
 Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 DL-hydroxyphenylhydantoin
-
 Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Agrobacterium tumefaciens
Ni2+ stimulates Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
 BQL9
-
Agrobacterium tumefaciens BQL9
-
 BQL9
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrouracil + H2O
-
 Agrobacterium tumefaciens ureidopropionate
-
 ?
dihydrouracil + H2O
-
 Agrobacterium tumefaciens BQL9 ureidopropionate
-
 ?
DL-hydroxyphenylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-hydroxyphenylglycine
-
 ?
DL-hydroxyphenylhydantoin + H2O
-
 Agrobacterium tumefaciens BQL9 N-carbamoyl-D-hydroxyphenylglycine
-
 ?
DL-isoproylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-valine
-
 ?
DL-isoproylhydantoin + H2O
-
 Agrobacterium tumefaciens BQL9 N-carbamoyl-D-valine
-
 ?
hydantoin + H2O
-
 Agrobacterium tumefaciens hydantoic acid
-
 ?
hydantoin + H2O
-
 Agrobacterium tumefaciens BQL9 hydantoic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, about 75% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
40
-
 30 min, about 65% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
50
-
 30 min, about 45% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
60
-
 30 min, about 35% of the activity that is retained after treatmet for 30 min at 4°C Agrobacterium tumefaciens
70
-
 30 min, about 20% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
80
-
 30 min, about 15% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7 9.7 reaction with dihydrouracil Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
8 9.7 pH 8.0: about 55% of maximal activity, pH 9.7: about 90% of maximal activity Agrobacterium tumefaciens