Cloned (Comment) | Organism |
---|---|
DL-hydantoinase cloned from 172-kb native plasmid | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | CuSO4*5H2O strongly inhibits L-methionine production | Geobacillus stearothermophilus | |
Cu2+ | - |
Pseudomonas fluorescens | |
Zn2+ | ZnSO4*7H2O strongly inhibits L-methionine production | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Bos taurus | |
Co2+ | stimulates production of L-methionine | Geobacillus stearothermophilus | |
Mn2+ | - |
Bos taurus | |
Mn2+ | - |
Thermus sp. | |
Mn2+ | stimulates production of L-methionine | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Geobacillus stearothermophilus |
155000 | 200000 | - |
Arthrobacter sp. |
200000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas fluorescens | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Arthrobacter sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Thermus sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Flavobacterium sp. | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Pseudomonas sp. NS671 | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Arthrobacter sp. DSM 3747 | - |
N-carbamoyl glycine | - |
? | |
hydantoin + H2O | Flavobacterium sp. AJ-3912 | - |
N-carbamoyl glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
DSM3747 | - |
Arthrobacter sp. DSM 3747 | - |
DSM3747 | - |
Bos taurus | - |
bovine | - |
Flavobacterium sp. | - |
AJ-3912 | - |
Flavobacterium sp. AJ-3912 | - |
AJ-3912 | - |
Geobacillus stearothermophilus | - |
strains NS1122A and LU1220 | - |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. NS671 | - |
- |
- |
Thermus sp. | - |
thermophilic bacterial strains, CBS 303.80 and CBS363.80, asporogenic gram-negative | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Pseudomonas fluorescens | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Thermus sp. | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Flavobacterium sp. | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Flavobacterium sp. AJ-3912 | 3-ureidopropionate | - |
? | |
5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus NS1122A | 3-ureidopropionate | - |
? | |
D-5-(1-methylpropyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-isoleucine | - |
? | |
D-5-(2-methylthioethyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-methionine | - |
? | |
D-5-benzylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-phenylalanine | - |
? | |
D-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-leucine | - |
? | |
D-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-D-leucine | - |
? | |
D-5-isopropylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-valine | - |
? | |
D-5-isopropylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-D-valine | - |
? | |
D-5-methylhydantoin + H2O | - |
Bos taurus | N-carbamoyl-D-alanine | - |
? | |
D-5-methylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-D-alanine | - |
? | |
D-5-methylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-D-alanine | - |
? | |
hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas fluorescens | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Arthrobacter sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Thermus sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Flavobacterium sp. | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Pseudomonas sp. NS671 | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Arthrobacter sp. DSM 3747 | N-carbamoyl glycine | - |
? | |
hydantoin + H2O | - |
Flavobacterium sp. AJ-3912 | N-carbamoyl glycine | - |
? | |
L-5-(1-methylpropyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-isoleucine | - |
? | |
L-5-(2-methylthioethyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-methionine | - |
? | |
L-5-benzylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-phenylalanine | - |
? | |
L-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-leucine | - |
? | |
L-5-isobutylhydantoin + H2O | - |
Geobacillus stearothermophilus NS1122A | N-carbamoyl-L-leucine | - |
? | |
L-5-methylhydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Bos taurus |
tetramer | - |
Pseudomonas fluorescens |
tetramer | 4 * 55000, SDS-PAGE | Geobacillus stearothermophilus |
tetramer | 4 * 55000-62000 | Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
L-5-methylhydantoin as substrate | Geobacillus stearothermophilus |
60 | 70 | resting cells | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas fluorescens |
8 | - |
resting cells | Geobacillus stearothermophilus |
9.5 | - |
- |
Pseudomonas putida |
9.5 | - |
L-5-methylhydantoin as substrate | Geobacillus stearothermophilus |