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Literature summary for 3.5.2.2 extracted from

  • Abendroth, J.; Chatterjee, S.; Schomburg, D.
    Purification of a D-hydantoinase using a laboratory-scale streamline phenyl column as the initial step (2000), J. Chromatogr., 737, 187-194.
    View publication on PubMed

Application

Application Comment Organism
synthesis used technically in combination with carbamoylases for production of D-amino acids and non-proteinogenic L-amino acids Thermus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Thermus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Thermus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ depend on Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Thermus sp.
50680
-
calculated from sequence Thermus sp.
100000
-
gel filtration Thermus sp.
113000
-
native protein, calculated from hydrodynamic radius Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
136.8
-
-
Thermus sp.

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Thermus sp.