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Literature summary for 3.5.2.2 extracted from
- A thermostable hydantoinase of Bacillus stearothermophilus NS1122A: Cloning, sequencing, and high expression of the enzyme gene, and some properties of the expressed enzyme (1994), Biosci. Biotechnol. Biochem., 58, 1621-1626.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Pseudomonas putida |
| medicine | industrial production of L-amino acids | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene for thermostable hydantoinase cloned and overexpressed in Escherichia coli JM105, vector pAHN134 | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | - |
Geobacillus stearothermophilus |  |
| 2,2'-dipyridyl | - |
Pseudomonas putida | |
| EDTA | inhibition 43% | Geobacillus stearothermophilus | |
| nitrilotriacetic acid | inhibition 42% | Geobacillus stearothermophilus | |
| o-phenanthroline | - |
Bos taurus | |
| o-phenanthroline | - |
Geobacillus stearothermophilus | |
| o-phenanthroline | - |
Pseudomonas putida | |
| p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
| p-hydroxymercuribenzoate | - |
Pseudomonas putida | |
| trans-1,2-cyclohexandiaminetetraacetic acid | inhibition 42% | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Bos taurus | |
| Co2+ | - |
Geobacillus stearothermophilus | |
| Mn2+ | - |
Bos taurus | |
| Mn2+ | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51720 | - |
calculated from amino acid sequence | Geobacillus stearothermophilus |
| 52000 | - |
4 * 52000, recombinant enzyme, SDS-PAGE | Geobacillus stearothermophilus |
| 60000 | - |
2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE | Geobacillus stearothermophilus |
| 62000 | - |
2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE | Geobacillus stearothermophilus |
| 110000 | - |
SDS-PAGE, room temperature, 2-mercaptoethanol | Geobacillus stearothermophilus |
| 200000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydantoin + H2O | Bos taurus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Geobacillus stearothermophilus | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas putida | - |
N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | Pseudomonas sp. NS671 | - |
N-carbamoyl glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
| Geobacillus stearothermophilus | - |
strains NS1122A and LU1220 | - |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. NS671 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6-dihydrouracil + H2O | - |
Bos taurus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Geobacillus stearothermophilus | 3-ureidopropionate | - |
? | |
| 5,6-dihydrouracil + H2O | - |
Pseudomonas putida | 3-ureidopropionate | - |
? | |
| hydantoin + H2O | - |
Bos taurus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas putida | N-carbamoyl glycine | - |
? | |
| hydantoin + H2O | - |
Pseudomonas sp. NS671 | N-carbamoyl glycine | - |
? | |
| L-5-(2-methylthioethyl)hydantoin + H2O | - |
Geobacillus stearothermophilus | N-carbamoyl-L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | 2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE | Geobacillus stearothermophilus |
| tetramer | - |
Bos taurus |
| tetramer | 4 * 52000, recombinant enzyme, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | fairly thermostable, oligomer retains full activity at 40-60°C, still retains 98% initial activity at 70°C | Geobacillus stearothermophilus |
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