Information on EC 3.5.2.2 - dihydropyrimidinase

New: Word Map on EC 3.5.2.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.2.2
-
RECOMMENDED NAME
GeneOntology No.
dihydropyrimidinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,6-dihydrouracil + H2O = 3-ureidopropanoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alanine metabolism
-
-
beta-Alanine metabolism
-
-
Drug metabolism - other enzymes
-
-
Metabolic pathways
-
-
Pantothenate and CoA biosynthesis
-
-
pyrimidine metabolism
-
-
Pyrimidine metabolism
-
-
thymine degradation
-
-
uracil degradation I (reductive)
-
-
SYSTEMATIC NAME
IUBMB Comments
5,6-dihydropyrimidine amidohydrolase
Also acts on dihydrothymine and hydantoin.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-74-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain 30148, 30199, 30200
-
-
Manually annotated by BRENDA team
strain 30149, 30155
-
-
Manually annotated by BRENDA team
strain 30149, 30155
-
-
Manually annotated by BRENDA team
strain 30201, 30203
-
-
Manually annotated by BRENDA team
strain IP-I 671
-
-
Manually annotated by BRENDA team
strain 30147, 30199, NRRL B-11291
-
-
Manually annotated by BRENDA team
strain 30150, 30205, 30206, 30207
-
-
Manually annotated by BRENDA team
strain 47C
-
-
Manually annotated by BRENDA team
strain CIP 67.1
-
-
Manually annotated by BRENDA team
DUL-DH101
-
-
Manually annotated by BRENDA team
NRRL B11291
-
-
Manually annotated by BRENDA team
Arthrobacter aurescens
DSM3747
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain MH602
UniProt
Manually annotated by BRENDA team
Bacillus sp. LU 1220
LU 1220
-
-
Manually annotated by BRENDA team
TS-23
SwissProt
Manually annotated by BRENDA team
strain JS-02
-
-
Manually annotated by BRENDA team
strain njut01
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
guinea-pig
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
strain 14.10
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Delftia sp. I24 DSM18833
-
-
-
Manually annotated by BRENDA team
strain S-5
-
-
Manually annotated by BRENDA team
strain S-5
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cat
-
-
Manually annotated by BRENDA team
AJ-3912
-
-
Manually annotated by BRENDA team
strain JC2310
-
-
Manually annotated by BRENDA team
strain GH-2
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
AJ3912
-
-
Manually annotated by BRENDA team
no activity in Agrobacterium tumefaciens
-
-
-
Manually annotated by BRENDA team
no activity in Agrobacterium tumefaciens 30204
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus alcalophilus
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida DSM 1693
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida DSM 549
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Ochrobactrum sp. G21 DSM18828
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Peptococcus anaerobius
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain 7711-2
-
-
Manually annotated by BRENDA team
strain CCRC 12857
SwissProt
Manually annotated by BRENDA team
strain RU-KM3
-
-
Manually annotated by BRENDA team
strain RU-KM3S
SwissProt
Manually annotated by BRENDA team
strain RUKM3s
-
-
Manually annotated by BRENDA team
Pseudomonas sp. AJ-11220
strain AJ-11220
-
-
Manually annotated by BRENDA team
strain NS671
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
strain 1.15
-
-
Manually annotated by BRENDA team
strain 1.15
-
-
Manually annotated by BRENDA team
strain CECT4114
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Streptomyces mitakaensis
-
-
-
Manually annotated by BRENDA team
wheat
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
hydantoinase and L-carbamoylase are involved in the conversion of DL-5-substituted hydantoins to an N-carbamyl intermediate that is subsequently converted to L-alpha-amino acids
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methylhydantoin + H2O
3-methylhydantoic acid
show the reaction diagram
2-imidazolidone + H2O
?
show the reaction diagram
-
-
-
-
?
2-thiohydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
2-thiouracil + H2O
?
show the reaction diagram
3-ethylphenylhydantoin + H2O
2-phenylhydantoic acid
show the reaction diagram
-
-
-
?
3-methylphenylhydantoin + H2O
2-phenylhydantoic acid
show the reaction diagram
-
-
-
?
3-phenylhydantoin + H2O
2-phenylhydantoic acid
show the reaction diagram
-
-
-
?
5,5-dimethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
5,6-dihydrothymine + H2O
3-(carbamoylamino)-2-methylpropanoic acid
show the reaction diagram
5,6-dihydrothymine + H2O
?
show the reaction diagram
5,6-dihydrothymine + H2O
N-carbamyl-beta-aminoisobutyrate
show the reaction diagram
5,6-dihydrouracil + H2O
3-ureidopropanoate
show the reaction diagram
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
5,6-dihydrouracil + H2O
?
show the reaction diagram
5,6-dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
5,6-dihydrouracil + H2O
N-carbamyl-beta-alanine
show the reaction diagram
5-(2-thienyl)-DL-hydantoin + H2O
N-carbamoyl-D-2-thienylglycine
show the reaction diagram
-
-
-
-
?
5-(4-hydroxyphenyl) hydantoin + H2O
N-carbamoyl-D-4-hydroxyphenylglycine
show the reaction diagram
5-(4-hydroxyphenyl)hydantoin + H2O
5-(4-hydroxyphenyl)-D-carbamoylate
show the reaction diagram
-
-
-
-
?
5-(p-hydroxybenzyl)hydantoin + H2O
?
show the reaction diagram
5-bromo-5,6-dihydrouracil + H2O
?
show the reaction diagram
5-fluoro-5,6-dihydrouracil + H2O
?
show the reaction diagram
5-fluorouracil + H2O
2-fluoro-3-ureidopropionic acid
show the reaction diagram
5-fluorouracil + hydantoin
2-fluoro-3-ureidopropionic acid + ?
show the reaction diagram
5-hydroxymethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
5-iodo-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
5-leucinyl-hydantoin + H2O
?
show the reaction diagram
5-phenylhydantoin + H2O
5-phenyl D-carbamoylate
show the reaction diagram
-
-
-
-
?
5-phenylhydantoin + H2O
D-phenylglycine + CO2 + NH3
show the reaction diagram
-
-
-
-
?
6-azauracil + H2O
?
show the reaction diagram
allantoin + H2O
?
show the reaction diagram
alpha-phenylsuccinimide + H2O
2-phenylsuccinamic acid
show the reaction diagram
beta-ureidoisobutyrate
dihydrothymine + H2O
show the reaction diagram
-
-
-
-
r
carbamoyl beta-aminoisobutyric acid + H2O
?
show the reaction diagram
D,L-benzylhydantoin + H2O
?
show the reaction diagram
D-2-phenylhydantoic acid + H2O
5-phenylhydantoin
show the reaction diagram
-
reverse reaction stereospecific, only D-isomer acts as substrate
-
-
r
D-5(p-hydroxybenzyl)-hydantoin + H2O
N-carbamoyl-D-tyrosine
show the reaction diagram
-
-
-
-
-
D-5-(1-methylpropyl)hydantoin + H2O
N-carbamoyl-D-isoleucine
show the reaction diagram
-
-
-
?
D-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
D-5-benzylhydantoin + H2O
N-carbamoyl-D-phenylalanine
show the reaction diagram
D-5-butylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-ethylhydantoin + H2O
?
show the reaction diagram
D-5-isobutylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-isobutylhydantoin + H2O
N-carbamoyl-D-leucine
show the reaction diagram
D-5-isopropylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
D-5-methyl-thio-ethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
show the reaction diagram
D-5-methylthioethylhydantoin + H2O
?
show the reaction diagram
D-5-propylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-benzylhydantoin
D-N-carbamoylphenylalanine
show the reaction diagram
D-benzylhydantoin + H2O
D-N-carbamoylphenylalanine
show the reaction diagram
D-methylhydantoin + H2O
?
show the reaction diagram
dihydroorotic acid + H2O
?
show the reaction diagram
-
-
-
-
?
dihydropyrimidine + H2O
N-carbamoyl amino acid
show the reaction diagram
dihydrothymidine + H2O
?
show the reaction diagram
Arthrobacter aurescens
-
-
-
-
?
dihydrothymine + H2O
N-carbamoyl-beta-aminoisobutyric acid
show the reaction diagram
-
-
-
-
?
dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
-
-
-
-
?
dihydrouracil + H2O
ureidopropionate
show the reaction diagram
dimethylhydantoin + H2O
?
show the reaction diagram
1.4% of the activity with dihydrouracil
-
-
?
DL-5(2-(methylthio)-ethyl)hydantoin + H2O
?
show the reaction diagram
DL-5-(2-carboxyethyl)hydantoin + H2O
L-glutamic acid
show the reaction diagram
-
-
exclusively L-form
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
DL-5-butylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-carboxyethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-cyanoethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
show the reaction diagram
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
DL-5-methyldihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-methylhydantoin
?
show the reaction diagram
DL-5-p-hydroxyphenylhydantoin + H2O
D-p-hydroxyphenylglycine
show the reaction diagram
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
DL-5-propylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-sec-butylhydantoin + H2O
?
show the reaction diagram
DL-5-thienylmethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-6-(4-chlorophenyl)-5,6-dihydrouracil + H2O
4-chloro-N-carbamoyl-beta-phenylalanine
show the reaction diagram
DL-6-isopropyldihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
DL-6-methyldihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
show the reaction diagram
DL-fluorobromylhydantoin + H2O
?
show the reaction diagram
Arthrobacter aurescens
-
-
-
-
?
DL-homophenylalanylhydantoin + H2O
?
show the reaction diagram
DL-hydroxymethylhydantoin + H2O
?
show the reaction diagram
DL-hydroxyphenylhydantoin + H2O
?
show the reaction diagram
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
DL-isoproylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
DL-methylthioethylhydantoin + H2O
?
show the reaction diagram
Arthrobacter aurescens
-
D-selective for the hydrolysis
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
?
show the reaction diagram
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-p-hydroxyphenylglycine
show the reaction diagram
7.2% of the activity with dihydrouracil
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
glutarimide + H2O
?
show the reaction diagram
hydantoin + H2O
?
show the reaction diagram
hydantoin + H2O
hydantoic acid
show the reaction diagram
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
hydantoin + H2O
ureidoacetic acid
show the reaction diagram
-
-
-
-
?
hydrothymine
carbamoyl beta-aminoisobutyric acid
show the reaction diagram
-
-
-
?
hydrouracil + H2O
N-carbamoyl beta-alanine
show the reaction diagram
-
-
-
?
L-5-(1-methylpropyl)hydantoin + H2O
N-carbamoyl-L-isoleucine
show the reaction diagram
-
-
-
?
L-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-L-methionine
show the reaction diagram
L-5-benzylhydantoin + H2O
N-carbamoyl-L-phenylalanine
show the reaction diagram
-
-
-
?
L-5-ethylhydantoin + H2O
?
show the reaction diagram
L-5-isobutylhydantoin + H2O
N-carbamoyl-L-leucine
show the reaction diagram
L-5-methylhydantoin + H2O
N-carbamoyl-L-alanine
show the reaction diagram
-
-
-
?
L-5-methylthioethylhydantoin + H2O
?
show the reaction diagram
L-benzylhydantoin
L-N-carbamoylphenylalanine
show the reaction diagram
m-chlorophenylhydantoin + H2O
?
show the reaction diagram
N-carbamoyl beta-alanine + H2O
5,6-dihydrouracil
show the reaction diagram
N-carbamoyl-beta-alanine + H2O
?
show the reaction diagram
N-carbamoyl-L-tryptophan + H2O
L-tryptophan + ?
show the reaction diagram
-
N-carbamoyl-D-tryptophan is no substrate
-
-
?
p-chlorophenylhydantoin + H2O
?
show the reaction diagram
p-methoxyphenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
p-methylphenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
phthalimide + H2O
?
show the reaction diagram
rac-5-p-fluorophenylhydantoin + H2O
?
show the reaction diagram
-
immobilized enzyme (linked to aminopropyl glass beads) converts 100%
-
-
?
rac-5-p-trifluoromethylphenylhydantoin + H2O
?
show the reaction diagram
-
immobilized enzyme (linked to aminopropyl glass beads) converts 87%
-
-
?
rac-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
immobilized enzyme (linked to aminopropyl glass beads) converts 100%
-
-
?
succinimide + H2O
?
show the reaction diagram
thymine + H2O
?
show the reaction diagram
-
-
-
-
?
uracil + H2O
?
show the reaction diagram
uric acid + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-