Information on EC 3.5.2.2 - dihydropyrimidinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.5.2.2
-
RECOMMENDED NAME
GeneOntology No.
dihydropyrimidinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
5,6-dihydrouracil + H2O = 3-ureidopropanoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
carboxylic acid amide hydrolysis
-
-
PATHWAY
KEGG Link
MetaCyc Link
beta-Alanine metabolism
-
Drug metabolism - other enzymes
-
Metabolic pathways
-
Pantothenate and CoA biosynthesis
-
Pyrimidine metabolism
-
thymine degradation
-
uracil degradation I (reductive)
-
SYSTEMATIC NAME
IUBMB Comments
5,6-dihydropyrimidine amidohydrolase
Also acts on dihydrothymine and hydantoin.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4,5-dihydropyrimidine amidohydrolase
-
-
4,5-dihydropyrimidine amidohydrolase
Rattus norvegicus Sprague-Dawley
-
-
-
bar9HYD
-
-
bar9HYD
-
-
-
CRMP
Drosophila melanogaster EP(3)3238
Q8IPQ2
-
-
D-hydantionase
-
-
D-hydantionase
Agrobacterium tumefaciens NRRL
-
-
-
D-hydantoinase
-
-
-
-
D-hydantoinase
Agrobacterium tumefaciens BQL9, Agrobacterium tumefaciens DUL-DH101
-
-
-
D-hydantoinase
Q84FR6
-
D-hydantoinase
Arthrobacter crystallopoietes DSM20117
Q84FR6
-
-
D-hydantoinase
-
-
D-hydantoinase
-
-
-
D-hydantoinase
-
-
D-hydantoinase
Burkholderia cepacia JS-02, Burkholderia cepacia njut01
-
-
-
D-hydantoinase
Q45515
-
D-hydantoinase
Geobacillus stearothermophilus SD1
-
-
-
D-hydantoinase
Q59699
-
D-hydantoinase
Q6TTR0
-
D-hydantoinase
Pseudomonas putida 7711-2
-
-
-
D-hydantoinase
Pseudomonas putida CCRC
Q59699
-
-
D-hydantoinase
Pseudomonas putida YZ-26
-, Q6TTR0
-
-
D-hydantoinase
-
-
DHP
-
-
-
-
DHP
Q56S49
-
DHP
Q56S49
-
-
DHP
Drosophila melanogaster EP(3)3238
Q8IPQ2
-
-
DHP
Q9P903
-
DHP
Q9EQF5
-
DHP
Q6Z744
-
DHP
Pseudomonas putida RU-KM3S
Q59699
-
-
DHP
Pseudomonas putida YZ-26
-
-
-
DHPase
-
-
Dihydropyrimidinase
-
-
-
-
Dihydropyrimidinase
Q59699
-
Dihydropyrimidinase
Pseudomonas putida RU-KM3S
Q59699
-
-
dihydropyrimidine amidohydrolase
-
-
dihydropyrimidine amidohydrolase
-
-
dihydropyrimidine amidohydrolase
-
-
dihydropyrimidine amidohydrolase
-
-
dihydropyrimidine amidohydrolase
-
-
dihydropyrimidine dehydrogenase
-
-
HDT
Agrobacterium tumefaciens NRRL
-
-
-
HYD
Q6TTR0
-
HYD
Pseudomonas putida YZ-26
Q6TTR0
-
-
hydantoin peptidase
-
-
-
-
hydantoinase
-
-
-
-
hydantoinase
-
-
hydantoinase
Agrobacterium tumefaciens NRRL B11291
-
-
-
hydantoinase
-
-
hydantoinase
P81006
-
hydantoinase
Q84FR6
-
hydantoinase
Arthrobacter crystallopoietes DSM20117
Q84FR6
-
-
hydantoinase
-
-
hydantoinase
Ensifer adhaerens S-5
-
-
-
hydantoinase
Q45515
-
hydantoinase
Microbacterium liquefaciens AJ3912
-
-
-
hydantoinase
-
-
hydantoinase
-
-
hydantoinase
Q59699
-
hydantoinase
Pseudomonas putida RU-KM3
-
-
-
hydantoinase
Pseudomonas putida RU-KM3S
Q59699
-
-
hydantoinase
Pseudomonas putida RUKM3s
-
-
-
hydantoinase
-
-
hydantoinase
-
-
hydantoinase
-
-
hydropyrimidine hydrase
-
-
-
-
hyuH
Arthrobacter crystallopoietes DSM20117
Q84FR6
-
-
hyuH
Microbacterium liquefaciens AJ3912
-
-
-
L-hydantoinase
-
-
microbial hydantoinase
-
-
P479
Pseudomonas putida YZ-26
-
-
-
PydB
Brevibacillus agri NCHU1002
-
-
-
pyrimidine hydrase
-
-
-
-
SmelDhp
Q0PQZ5
possesses both hydantoinase and dihydropyrimidinase activities
SmelDhp
Sinorhizobium meliloti CECT4114
Q0PQZ5
possesses both hydantoinase and dihydropyrimidinase activities
-
thHYD
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-74-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 30148, 30199, 30200
-
-
Manually annotated by BRENDA team
Agrobacterium rhizogenes 30148
strain 30148, 30199, 30200
-
-
Manually annotated by BRENDA team
strain 30149, 30155
-
-
Manually annotated by BRENDA team
Agrobacterium rubi 30149
strain 30149, 30155
-
-
Manually annotated by BRENDA team
strain 30201, 30203
-
-
Manually annotated by BRENDA team
Agrobacterium sp. 30201
strain 30201, 30203
-
-
Manually annotated by BRENDA team
Agrobacterium sp. IP-I 671
strain IP-I 671
-
-
Manually annotated by BRENDA team
; strain CIP 67.1
-
-
Manually annotated by BRENDA team
strain 30147, 30199, NRRL B-11291
-
-
Manually annotated by BRENDA team
strain 30147, 30199, NRRL B-11291; strain 30150, 30205, 30206, 30207
-
-
Manually annotated by BRENDA team
strain NRRL B 11291
-
-
Manually annotated by BRENDA team
strain NRRL B11291
-
-
Manually annotated by BRENDA team
strain RU-AE01
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens 30147
strain 30147, 30199, NRRL B-11291
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens 30150
strain 30150, 30205, 30206, 30207
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens 47C
strain 47C
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens BQL9
BQL9
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens BQL9
strain BQL9
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens CIP 67.1
strain CIP 67.1
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens DUL-DH101
DUL-DH101
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens NRRL
strain NRRL B 11291
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens NRRL
strain NRRL B11291
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens NRRL B11291
NRRL B11291
-
-
Manually annotated by BRENDA team
strain DSM20117
SwissProt
Manually annotated by BRENDA team
Arthrobacter crystallopoietes DSM20117
-
-
-
Manually annotated by BRENDA team
Arthrobacter crystallopoietes DSM20117
strain DSM20117
SwissProt
Manually annotated by BRENDA team
Arthrobacter sp. DSM3747
DSM3747
-
-
Manually annotated by BRENDA team
strain MH602
UniProt
Manually annotated by BRENDA team
LU 1220
-
-
Manually annotated by BRENDA team
strain AR9
-
-
Manually annotated by BRENDA team
TS-23
SwissProt
Manually annotated by BRENDA team
strain AR9
-
-
Manually annotated by BRENDA team
Bacillus sp. LU 1220
LU 1220
-
-
Manually annotated by BRENDA team
TS-23
SwissProt
Manually annotated by BRENDA team
beef
-
-
Manually annotated by BRENDA team
beef; calf
-
-
Manually annotated by BRENDA team
bovine; calf
-
-
Manually annotated by BRENDA team
calf; ox
-
-
Manually annotated by BRENDA team
strain NCHU1002
-
-
Manually annotated by BRENDA team
Brevibacillus agri NCHU1002
strain NCHU1002
-
-
Manually annotated by BRENDA team
L-specific hydantoinase
-
-
Manually annotated by BRENDA team
strain JS-02
-
-
Manually annotated by BRENDA team
strain njut01
-
-
Manually annotated by BRENDA team
Burkholderia cepacia JS-02
strain JS-02
-
-
Manually annotated by BRENDA team
Burkholderia cepacia njut01
strain njut01
-
-
Manually annotated by BRENDA team
guinea-pig
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
Corynebacterium pseudodiphtheriticum 14.10
strain 14.10
-
-
Manually annotated by BRENDA team
Delftia sp. I24 DSM18833
-
-
-
Manually annotated by BRENDA team
mutant supA4; strain EP(3)3238 CRMPsup mutants
SwissProt
Manually annotated by BRENDA team
strain EP(3)3238 CRMPsup mutants
SwissProt
Manually annotated by BRENDA team
Drosophila melanogaster EP(3)3238
mutant supA4; strain EP(3)3238 CRMPsup mutants
SwissProt
Manually annotated by BRENDA team
Drosophila melanogaster EP(3)3238
strain EP(3)3238 CRMPsup mutants
SwissProt
Manually annotated by BRENDA team
strain S-5
-
-
Manually annotated by BRENDA team
Ensifer adhaerens S-5
strain S-5
-
-
Manually annotated by BRENDA team
I-3, FERM-P no. 6901, facultively anaerobic, non-spore forming, gram-negative, motile with peritrichous flagella
-
-
Manually annotated by BRENDA team
Flavobacterium sp. AJ-3912
AJ-3912
-
-
Manually annotated by BRENDA team
strain SD-1; strains NS1122A and LU1220
-
-
Manually annotated by BRENDA team
strains NS1122A and LU1220
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus JC2310
strain JC2310
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus SD-1
strain SD-1
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus SD1
SD1
-
-
Manually annotated by BRENDA team
Geobacillus thermocatenulatus GH-2
strain GH-2
-
-
Manually annotated by BRENDA team
Microbacterium liquefaciens AJ3912
AJ3912
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
no activity in Agrobacterium tumefaciens
-
-
-
Manually annotated by BRENDA team
no activity in Agrobacterium tumefaciens 30204
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus alcalophilus
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida DSM 1693
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas putida DSM 549
-
-
-
Manually annotated by BRENDA team
Ochrobactrum sp. G21 DSM18828
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Peptococcus anaerobius
-
-
-
Manually annotated by BRENDA team
strains 1.2 and 1.9
-
-
Manually annotated by BRENDA team
; DSM 84, formerly classified as P. fluorescens; strains 2.2, 2.5
-
-
Manually annotated by BRENDA team
; strain 77
-
-
Manually annotated by BRENDA team
biotype B, strain ATCC 17536
-
-
Manually annotated by BRENDA team
DSM 84, formerly classified as P. fluorescens
-
-
Manually annotated by BRENDA team
formerly classified as P. striata
-
-
Manually annotated by BRENDA team
strain 7711-2
-
-
Manually annotated by BRENDA team
strain CCRC 12857
SwissProt
Manually annotated by BRENDA team
strain RU-KM3
-
-
Manually annotated by BRENDA team
strain RU-KM3S
SwissProt
Manually annotated by BRENDA team
strain RUKM3s
-
-
Manually annotated by BRENDA team
strain YZ-26
SwissProt
Manually annotated by BRENDA team
strain YZ-26
-
-
Manually annotated by BRENDA team
YZ-26
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida 7711-2
strain 7711-2
-
-
Manually annotated by BRENDA team
Pseudomonas putida CCRC
strain CCRC 12857
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida RU-KM3
strain RU-KM3
-
-
Manually annotated by BRENDA team
Pseudomonas putida RU-KM3S
strain RU-KM3S
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida RUKM3s
strain RUKM3s
-
-
Manually annotated by BRENDA team
Pseudomonas putida YZ-26
strain YZ-26
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida YZ-26
strain YZ-26
-
-
Manually annotated by BRENDA team
Pseudomonas putida YZ-26
YZ-26
SwissProt
Manually annotated by BRENDA team
strain AJ-11220
-
-
Manually annotated by BRENDA team
strain NS671
-
-
Manually annotated by BRENDA team
Pseudomonas sp. AJ-11220
strain AJ-11220
-
-
Manually annotated by BRENDA team
rat; strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
strain Sprague-Dawley
-
-
Manually annotated by BRENDA team
strain 1.15
-
-
Manually annotated by BRENDA team
Serratia liquefaciens 1.15
strain 1.15
-
-
Manually annotated by BRENDA team
strain CECT4114
UniProt
Manually annotated by BRENDA team
strain CECT4114
-
-
Manually annotated by BRENDA team
Sinorhizobium meliloti CECT4114
strain CECT4114
UniProt
Manually annotated by BRENDA team
Streptomyces mitakaensis
-
-
-
Manually annotated by BRENDA team
pig
-
-
Manually annotated by BRENDA team
thermophilic bacterial strains, CBS 303.80 and CBS363.80, asporogenic gram-negative
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
A1E351, -
hydantoinase and L-carbamoylase are involved in the conversion of DL-5-substituted hydantoins to an N-carbamyl intermediate that is subsequently converted to L-alpha-amino acids
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methylhydantoin + H2O
3-methylhydantoic acid
show the reaction diagram
-
-
-
-
?
1-methylhydantoin + H2O
3-methylhydantoic acid
show the reaction diagram
-
-
-
?
1-methylhydantoin + H2O
3-methylhydantoic acid
show the reaction diagram
-
poor substrate
-
-
?
2-imidazolidone + H2O
?
show the reaction diagram
-
-
-
-
?
2-thiohydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
2-thiouracil + H2O
?
show the reaction diagram
Agrobacterium sp., Agrobacterium sp. IP-I 671
-
-
-
-
?
3-ethylphenylhydantoin + H2O
2-phenylhydantoic acid
show the reaction diagram
-
-
-
?
3-methylphenylhydantoin + H2O
2-phenylhydantoic acid
show the reaction diagram
-
-
-
?
3-phenylhydantoin + H2O
2-phenylhydantoic acid
show the reaction diagram
-
-
-
?
4-chloro-DL-6-phenyl-5,6-dihydrouracil + H2O
4-chloro-N-rac-carbamoyl-beta-phenylalanine
show the reaction diagram
Arthrobacter crystallopoietes, Ochrobactrum sp. G21, Delftia sp. I24, Ochrobactrum sp. G21 DSM18828, Arthrobacter crystallopoietes DSM20117, Delftia sp. I24 DSM18833
-
-
-
-
?
5,5-dimethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
r
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
r
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
-
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
-
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
-
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
Q0PQZ5, -
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
-
5,6-dihydrothymine + H2O
?
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
Sinorhizobium meliloti CECT4114
Q0PQZ5
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
5,6-dihydrothymine + H2O
3-(carbamoylamino)-2-methylpropanoic acid
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
N-carbamyl-beta-aminoisobutyrate
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
N-carbamyl-beta-aminoisobutyrate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-, ?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-, ?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Peptococcus anaerobius
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Q0PQZ5, -
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Q28SA7
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
purified preparation demonstrates no activity on this substrate
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium sp. 30201
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium rubi 30149
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-, purified preparation demonstrates no activity on this substrate
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Serratia liquefaciens 1.15
-
-
-
-
-, ?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens CIP 67.1
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium rhizogenes 30148
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens 30150
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Sinorhizobium meliloti CECT4114
Q0PQZ5
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Geobacillus stearothermophilus JC2310
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Corynebacterium pseudodiphtheriticum 14.10
-
-
-
-
-, ?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens 47C
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium sp. 1-671
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Flavobacterium sp. AJ-3912, Geobacillus stearothermophilus NS1122A
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens 30147
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropanoate
show the reaction diagram
-
26% relative enzyme activity
-
-
?
5,6-dihydrouracil + H2O
?
show the reaction diagram
-, Q59699
2.35fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
5,6-dihydrouracil + H2O
?
show the reaction diagram
-, Q56S49
highest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
5,6-dihydrouracil + H2O
?
show the reaction diagram
Q56S49
highest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
5,6-dihydrouracil + H2O
?
show the reaction diagram
Pseudomonas putida RU-KM3S
Q59699
2.35fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
5,6-dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
Pseudomonas putida, Pseudomonas putida YZ-26
-
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamyl-beta-alanine
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamyl-beta-alanine
show the reaction diagram
-
-
-
-
?
5-(2-thienyl)-DL-hydantoin + H2O
N-carbamoyl-D-2-thienylglycine
show the reaction diagram
-
-
-
-
?
5-(4-hydroxyphenyl)hydantoin + H2O
5-(4-hydroxyphenyl)-D-carbamoylate
show the reaction diagram
-
-
-
-
?
5-(p-hydroxybenzyl)hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
5-(p-hydroxybenzyl)hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
5-bromo-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
5-bromo-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
r
5-bromo-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
-
5-bromo-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
r
5-fluoro-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
-
5-fluoro-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
less effective substrate
-
-
?
5-fluorouracil + H2O
2-fluoro-3-ureidopropionic acid
show the reaction diagram
-, Q59699
growth of GMP1 and GMP2 mutants but not of wild-type RU-KM3S, mutants independent of inducer
-
-
?
5-fluorouracil + H2O
2-fluoro-3-ureidopropionic acid
show the reaction diagram
Pseudomonas putida RU-KM3S
Q59699
growth of GMP1 and GMP2 mutants but not of wild-type RU-KM3S, mutants independent of inducer
-
-
?
5-fluorouracil + hydantoin
2-fluoro-3-ureidopropionic acid + ?
show the reaction diagram
-, Q59699
growth of GMP1 and GMP2 mutants and wild-type RU-KM3S
-
-
?
5-fluorouracil + hydantoin
2-fluoro-3-ureidopropionic acid + ?
show the reaction diagram
Pseudomonas putida RU-KM3S
Q59699
growth of GMP1 and GMP2 mutants and wild-type RU-KM3S
-
-
?
5-hydroxymethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
5-iodo-5,6-dihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
5-leucinyl-hydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens NRRL B11291
-
-
-
-
?
5-phenylhydantoin + H2O
5-phenyl D-carbamoylate
show the reaction diagram
-
-
-
-
?
5-phenylhydantoin + H2O
D-phenylglycine + CO2 + NH3
show the reaction diagram
-
-
-
-
?
6-azauracil + H2O
?
show the reaction diagram
Agrobacterium sp., Agrobacterium sp. IP-I 671
-
-
-
-
?
allantoin + H2O
?
show the reaction diagram
-
-
-
-
?
allantoin + H2O
?
show the reaction diagram
-
-
-
-
?
allantoin + H2O
?
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
alpha-phenylsuccinimide + H2O
2-phenylsuccinamic acid
show the reaction diagram
-
-
-
-
?
beta-ureidoisobutyrate
dihydrothymine + H2O
show the reaction diagram
-
-
-
-
r
carbamoyl beta-aminoisobutyric acid + H2O
?
show the reaction diagram
-
-
-
-
?
carbamoyl beta-aminoisobutyric acid + H2O
?
show the reaction diagram
-
-
-
-
?
D,L-benzylhydantoin + H2O
?
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia JS-02
-
-
-
-
?
D-2-phenylhydantoic acid + H2O
5-phenylhydantoin
show the reaction diagram
-
reverse reaction stereospecific, only D-isomer acts as substrate
-
-
r
D-5(p-hydroxybenzyl)-hydantoin + H2O
N-carbamoyl-D-tyrosine
show the reaction diagram
-
-
-
-
-
D-5-(1-methylpropyl)hydantoin + H2O
N-carbamoyl-D-isoleucine
show the reaction diagram
-
-
-
?
D-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
-
D-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
?
D-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
-
D-5-benzylhydantoin + H2O
N-carbamoyl-D-phenylalanine
show the reaction diagram
-
-
-
-
?
D-5-benzylhydantoin + H2O
N-carbamoyl-D-phenylalanine
show the reaction diagram
-
-
-
?
D-5-butylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-ethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-ethylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens BQL9
-
-
-
-
r
D-5-isobutylhydantoin + H2O
N-carbamoyl-D-leucine
show the reaction diagram
Geobacillus stearothermophilus, Geobacillus stearothermophilus NS1122A
-
-
-
?
D-5-isobutylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Geobacillus stearothermophilus, Geobacillus stearothermophilus NS1122A
-
-
-
?
D-5-isopropylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-methyl-thio-ethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
show the reaction diagram
-
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
show the reaction diagram
-
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
show the reaction diagram
-
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
show the reaction diagram
-
L-5-methylhydantoin is no substrate
-
-
?
D-5-methylhydantoin + H2O
N-carbamoyl-D-alanine
show the reaction diagram
Geobacillus stearothermophilus NS1122A
-
-
-
?
D-5-methylthioethylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens BQL9
-
-
-
-
r
D-5-propylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
D-benzylhydantoin
D-N-carbamoylphenylalanine
show the reaction diagram
Microbacterium liquefaciens, Microbacterium liquefaciens AJ3912
-
-
-
-
?
D-benzylhydantoin + H2O
D-N-carbamoylphenylalanine
show the reaction diagram
A1E351, -
-
-
-
?
D-methylhydantoin + H2O
?
show the reaction diagram
-, Q59699
1.57fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
D-methylhydantoin + H2O
?
show the reaction diagram
Pseudomonas putida RU-KM3S
Q59699
1.57fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
dihydroorotic acid + H2O
?
show the reaction diagram
-
-
-
-
?
dihydropyrimidine + H2O
N-carbamoyl amino acid
show the reaction diagram
-
-
-
-
r
dihydropyrimidine + H2O
N-carbamoyl amino acid
show the reaction diagram
-
-
-
-
r
dihydropyrimidine + H2O
N-carbamoyl amino acid
show the reaction diagram
-
-
-
-
?
dihydropyrimidine + H2O
N-carbamoyl amino acid
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
dihydrothymidine + H2O
?
show the reaction diagram
-
-
-
-
?
dihydrothymine + H2O
N-carbamoyl-beta-aminoisobutyric acid
show the reaction diagram
-
-
-
-
?
dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
-
-
-
-
?
dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens NRRL B11291
-
-
-
-
?
dihydrouracil + H2O
ureidopropionate
show the reaction diagram
-
-
-
-
?
dihydrouracil + H2O
ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens BQL9
-
-
-
-
?
dimethylhydantoin + H2O
?
show the reaction diagram
Q28SA7
1.4% of the activity with dihydrouracil
-
-
?
DL-5(2-(methylthio)-ethyl)hydantoin + H2O
?
show the reaction diagram
-, Q56S49
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
DL-5(2-(methylthio)-ethyl)hydantoin + H2O
?
show the reaction diagram
Q56S49
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
DL-5-(2-carboxyethyl)hydantoin + H2O
L-glutamic acid
show the reaction diagram
-
-
exclusively L-form
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
-
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
-
steric configuration D
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
strictly D-selective
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
-
the enzyme exhibits strict D-enantioselectivity for DL-5-(2-methylthioethyl)-hydantoin
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Agrobacterium sp. 30201
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Brevibacillus agri NCHU1002
-
the enzyme exhibits strict D-enantioselectivity for DL-5-(2-methylthioethyl)-hydantoin
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Agrobacterium tumefaciens CIP 67.1
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Agrobacterium tumefaciens 30150
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
DL-5-(2-methylthioethyl)-hydantoin + H2O
N-carbamoyl-D-methionine
show the reaction diagram
Agrobacterium tumefaciens 30147
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-, Q59699
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
-
produces stereospecifically D-p-hydroxyphenylglycine
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Pseudomonas putida 7711-2
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Pseudomonas putida CCRC
Q59699
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
DL-5-(p-hydroxyphenyl)hydantoin + H2O
N-carbamoyl-p-hydroxyphenylglycine
show the reaction diagram
Agrobacterium sp. 1-671
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
poorly hydrolyzed
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
-
only slight activity
-
-
?
DL-5-benzylhydantoin + H2O
?
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
DL-5-butylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-carboxyethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-cyanoethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
show the reaction diagram
-
-
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
show the reaction diagram
-
-
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
show the reaction diagram
-
-
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
show the reaction diagram
-
strictly L-selective for the cleavage
-
-
?
DL-5-indolylmethylhydantoin + H2O
L-tryptophan
show the reaction diagram
-
strictly D-selective
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens CIP 67.1
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens 47C
-
-
-
-
?
DL-5-isobutyl-hydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Q0PQZ5, -
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
only D-isomer hydrolyzed
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
-
only D-isomer hydrolyzed
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium sp. 30201
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium tumefaciens CIP 67.1
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium tumefaciens 30150
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Sinorhizobium meliloti CECT4114
Q0PQZ5
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium tumefaciens 47C
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
DL-5-isopropylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium tumefaciens 30147
-
-
-
-
?
DL-5-methyldihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-methylhydantoin
?
show the reaction diagram
-
-
-
-
-
DL-5-methylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-methylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-methylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-p-hydroxyphenylhydantoin + H2O
D-p-hydroxyphenylglycine
show the reaction diagram
Ensifer adhaerens, Ensifer adhaerens S-5
-
hydantoinase and carbamylase involved
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
-
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
r
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Q28SA7
45% of the activity with dihydrouracil
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Geobacillus stearothermophilus JC2310
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
DL-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Pseudomonas putida YZ-26
-
-
-
-
?
DL-5-propylhydantoin
?
show the reaction diagram
-
-
-
-
?
DL-5-sec-butylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-sec-butylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-5-sec-butylhydantoin + H2O
?
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
DL-5-sec-butylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens CIP 67.1
-
-
-
-
?
DL-5-sec-butylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens 47C
-
-
-
-
?
DL-5-thienylmethylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-6-isopropyldihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
DL-6-methyldihydrouracil + H2O
?
show the reaction diagram
-
-
-
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
show the reaction diagram
-
-
27% conversion. Enzyme preferably hydrolyzes L-Phe-5,6-dihydrouracil to L-N-carbamoyl-beta-phenylalanine, 61% enantiomeric excess of L-enantiomer
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
show the reaction diagram
Ochrobactrum sp. G21, Delftia sp. I24, Ochrobactrum sp. G21 DSM18828
-
-
enzyme does not display a significant entantioselectivity
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
show the reaction diagram
Arthrobacter crystallopoietes DSM20117
-
-
27% conversion. Enzyme preferably hydrolyzes L-Phe-5,6-dihydrouracil to L-N-carbamoyl-beta-phenylalanine, 61% enantiomeric excess of L-enantiomer
-
?
DL-6-phenyl-5,6-dihydrouracil + H2O
N-rac-carbamoyl-beta-phenylalanine
show the reaction diagram
Delftia sp. I24 DSM18833
-
-
enzyme does not display a significant entantioselectivity
-
?
DL-fluorobromylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
DL-homophenylalanylhydantoin + H2O
?
show the reaction diagram
Brevibacillus agri, Brevibacillus agri NCHU1002
-
the enzyme exhibits non-enantiospecificity for DL-homophenylalanylhydantoin
-
-
?
DL-hydroxymethylhydantoin + H2O
?
show the reaction diagram
Geobacillus stearothermophilus, Geobacillus stearothermophilus SD1
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
Agrobacterium tumefaciens BQL9
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
Geobacillus stearothermophilus SD1
-
-
-
-
?
DL-hydroxyphenylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens NRRL
-
-
-
-
?
DL-isoproylhydantoin + H2O
N-carbamoyl-D-valine
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens BQL9
-
-
-
-
?
DL-methylthioethylhydantoin + H2O
?
show the reaction diagram
-
D-selective for the hydrolysis
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
-
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
19% relative enzyme activity
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
Brevibacillus agri, Brevibacillus agri NCHU1002
-
the enzyme exhibits strict D-enantioselectivity for DL-p-hydroxyphenylhydantoin
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
-
19% relative enzyme activity
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-hydroxyphenylglycine
show the reaction diagram
Pseudomonas putida YZ-26
-
-
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
?
show the reaction diagram
-, Q56S49
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
?
show the reaction diagram
Q56S49
EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
DL-p-hydroxyphenylhydantoin + H2O
N-carbamoyl-D-p-hydroxyphenylglycine
show the reaction diagram
Q28SA7
7.2% of the activity with dihydrouracil
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
-
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
39.9% relative enzyme activity
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
Geobacillus stearothermophilus SD1
-
-
-
-
?
DL-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
39.9% relative enzyme activity
-
-
?
glutarimide + H2O
?
show the reaction diagram
-
-
-
-
?
glutarimide + H2O
?
show the reaction diagram
-
-
-
-
?
glutarimide + H2O
?
show the reaction diagram
-
-
-
-
-
glutarimide + H2O
?
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
r
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Peptococcus anaerobius
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
poorly hydrolyzed
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Q28SA7
18.7% of the activity with dihydrouracil
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Agrobacterium tumefaciens BQL9
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Agrobacterium tumefaciens NRRL B11291
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Geobacillus stearothermophilus SD1
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Geobacillus stearothermophilus JC2310
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
hydantoin + H2O
hydantoic acid
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
r
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
ir
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
r
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Peptococcus anaerobius
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-, Q59699
4fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
preferred substrate, 100% relative enzyme activity
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium sp. 30201
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Arthrobacter sp. DSM3747
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Arthrobacter sp. DSM3747
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium rubi 30149
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Serratia liquefaciens 1.15
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Pseudomonas sp. AJ-11220
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium rhizogenes 30148
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium tumefaciens NRRL
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium tumefaciens 30150
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus JC2310
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Corynebacterium pseudodiphtheriticum 14.10
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
preferred substrate, 100% relative enzyme activity
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium sp. 1-671
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Pseudomonas putida YZ-26
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Pseudomonas putida RU-KM3S
Q59699
4fold elevated hydantoinase activity in uninduced mutant cells compared with the wild type, but equivalent enzyme activity in induced mutant and wild type cells
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Flavobacterium sp. AJ-3912
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium tumefaciens 30147
-
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
Q6TTR0
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
Pseudomonas putida RU-KM3
-
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
Pseudomonas putida YZ-26
-
-
-
-
-
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
Pseudomonas putida YZ-26
Q6TTR0
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
Pseudomonas putida YZ-26
-
-
-
-
?
hydantoin + H2O
N-carbamoylglycine
show the reaction diagram
Pseudomonas putida YZ-26
Q6TTR0
-
-
-
?
hydantoin + H2O
?
show the reaction diagram
-, Q56S49
lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
hydantoin + H2O
?
show the reaction diagram
Q56S49
lowest activity of the EDTA-dialyzed/Co2+-substituted enzyme
-
-
?
hydantoin + H2O
ureidoacetic acid
show the reaction diagram
-
-
-
-
?
hydrothymine
carbamoyl beta-aminoisobutyric acid
show the reaction diagram
-
-
-
?
hydrouracil + H2O
N-carbamoyl beta-alanine
show the reaction diagram
-
-
-
?
L-5-(1-methylpropyl)hydantoin + H2O
N-carbamoyl-L-isoleucine
show the reaction diagram
-
-
-
?
L-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-L-methionine
show the reaction diagram
-
-
-
?
L-5-(2-methylthioethyl)hydantoin + H2O
N-carbamoyl-L-methionine
show the reaction diagram
-
-
-
?
L-5-benzylhydantoin + H2O
N-carbamoyl-L-phenylalanine
show the reaction diagram
-
-
-
?
L-5-ethylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens BQL9
-
-
-
-
r
L-5-isobutylhydantoin + H2O
N-carbamoyl-L-leucine
show the reaction diagram
Geobacillus stearothermophilus, Geobacillus stearothermophilus NS1122A
-
-
-
?
L-5-methylhydantoin + H2O
N-carbamoyl-L-alanine
show the reaction diagram
-
-
-
?
L-5-methylthioethylhydantoin + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens BQL9
-
-
-
-
r
L-benzylhydantoin
L-N-carbamoylphenylalanine
show the reaction diagram
Microbacterium liquefaciens, Microbacterium liquefaciens AJ3912
-
-
-
-
?
m-chlorophenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
m-chlorophenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
N-carbamoyl beta-alanine + H2O
5,6-dihydrouracil
show the reaction diagram
-
-
-
-
r
N-carbamoyl beta-alanine + H2O
5,6-dihydrouracil
show the reaction diagram
-
-
-
-
r
N-carbamoyl beta-alanine + H2O
5,6-dihydrouracil
show the reaction diagram
-
-
-
-
r
N-carbamoyl-beta-alanine + H2O
?
show the reaction diagram
-
-
-
-
?
N-carbamoyl-L-tryptophan + H2O
L-tryptophan + ?
show the reaction diagram
-
N-carbamoyl-D-tryptophan is no substrate
-
-
?
p-chlorophenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
p-chlorophenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
p-chlorophenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
p-methoxyphenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
p-methylphenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
phthalimide + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens NRRL B11291
-
low activity
-
-
?
rac-5-p-fluorophenylhydantoin + H2O
?
show the reaction diagram
-
immobilized enzyme (linked to aminopropyl glass beads) converts 100%
-
-
?
rac-5-p-trifluoromethylphenylhydantoin + H2O
?
show the reaction diagram
-
immobilized enzyme (linked to aminopropyl glass beads) converts 87%
-
-
?
rac-5-phenylhydantoin + H2O
N-carbamoyl-D-phenylglycine
show the reaction diagram
-
immobilized enzyme (linked to aminopropyl glass beads) converts 100%
-
-
?
succinimide + H2O
?
show the reaction diagram
-
-
-
-
-
succinimide + H2O
?
show the reaction diagram
-
-
-
-
-
succinimide + H2O
?
show the reaction diagram
-
-
-
-
r
thymine + H2O
?
show the reaction diagram
-
-
-
-
?
uracil + H2O
?
show the reaction diagram
Agrobacterium sp., Agrobacterium sp. IP-I 671
-
-
-
-
?
uric acid + H2O
?
show the reaction diagram
-
-
-
-
?
m-chlorophenylhydantoin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
DL-5-phenylhydantoin is no substrate
-
-
-
additional information
?
-
-
L-dihydroorotic acid is no substrate
-
-
-
additional information
?
-
-
diphenylhydantoin and 5,5-disubstituted hydantoins like 5-ethyl-5-phenylhydantoin, 5,5-diphenylhydantoin, D-3-methyl-5-phenylhydantoin, D-3-ethyl-5-phenylhydantoin, alpha-methyl-alpha-phenylsuccinimide, N-methyl-alpha-phenylsuccinimide and alpha-ethyl-alpha-phenylglutarimide are no substrates
-
-
-
additional information
?
-
-
hydantoin, urea, allantoin and dihydroorotate are no substrates
-
-
-
additional information
?
-
-
5,5-dimethylhydantoin is no substrate
-
-
-
additional information
?
-
-
succinimide, dihydro-L-orotic-2-acid, dihydrouridine and dihydrothymidine are no substrates
-
-
-
additional information
?
-
-
inactive with carbamoyl beta-alanine amide, barbituric acid, imidazole 4,5-dicarboxylic acid, 5-(carboxymethylidine)hydantoin, orotic acid, 4,5-aminoimidazolecarboximide, urocanic acid and 5-(diphenyl)hydantoin
-
-
-
additional information
?
-
-
disubstituted hydantoins or 1-substituted hydantoins are no substrates
-
-
-
additional information
?
-
-
hydantoins derived from alanine, valine, leucine, serine, phenylalanine and hydroxyphenylglycine are substrates, hydantoins derived from aspartic acid, lysine, and 5,5-disubstituted hydantoins are not hydrolyzed
-
-
-
additional information
?
-
-
DL-hydroxymethylhydantoin and 5,5-disubstituted hydantoin derivatives are no substrates
-
-
-
additional information
?
-
Q2XST3, Q8IPQ2
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
-
does not hydrolyze hydantoins
-
-
-
additional information
?
-
-
no activity towards uracil and succinimide
-
-
-
additional information
?
-
-
catalyzes the hydrolysis of 5-monosubstituted hydantoin to enantionmeric N-carbamoyl-amino acids
-
-
-
additional information
?
-
-
enzyme presents both hydantoinase and dihydropyrimidinase activities, with higher affinity for the natural six-membered ring substrates. For the five-membered ring substrates, affinity is greater for those with aliphatic and apolar groups in the 5th carbon atom, with the highest rates of hydrolysis for D-5-methyl and D-5-ethyl hydantoin. Enzyme is enantioselective for the D-isomer of monosubstituted 5-hydantoins. Activity with substrate analogues such as allantoin, 5,5-dimethylhydantoin, 5-benzyl,5-methyl-hydantoin, succinimide, rodamine or tiazoledione is not detected under the conditions assayed
-
-
-
additional information
?
-
Q28SA7
no substrate: diphenylhydantoin, 5-(hydroxymethyl)uracil, benzylhydantoin, isopropylhydantoin
-
-
-
additional information
?
-
Rattus norvegicus Sprague-Dawley
-
diphenylhydantoin and 5,5-disubstituted hydantoins like 5-ethyl-5-phenylhydantoin, 5,5-diphenylhydantoin, D-3-methyl-5-phenylhydantoin, D-3-ethyl-5-phenylhydantoin, alpha-methyl-alpha-phenylsuccinimide, N-methyl-alpha-phenylsuccinimide and alpha-ethyl-alpha-phenylglutarimide are no substrates
-
-
-
additional information
?
-
Drosophila melanogaster EP(3)3238
Q2XST3, Q8IPQ2
at 0 and 0.01 mM dietary 5-fluorouracil, CRMPsup mutants emerged in numbers similar to those of CRMP+ hemizygotes, survival of CRMPsup mutants was diminished at 0.03 mM 5-fluorouracil
-
-
?
additional information
?
-
Agrobacterium tumefaciens 47C
-
disubstituted hydantoins or 1-substituted hydantoins are no substrates
-
-
-
additional information
?
-
-
DL-hydroxymethylhydantoin and 5,5-disubstituted hydantoin derivatives are no substrates
-
-
-
additional information
?
-
Pseudomonas putida YZ-26
-
catalyzes the hydrolysis of 5-monosubstituted hydantoin to enantionmeric N-carbamoyl-amino acids
-
-
-
additional information
?
-
Pseudomonas putida YZ-26
-
no activity towards uracil and succinimide
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
r
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
r
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
?
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
-
5,6-dihydropyrimidine + H2O
beta-ureido acid
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
-
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
-
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
r
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
-
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
r
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Peptococcus anaerobius
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
purified preparation demonstrates no activity on this substrate
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium rubi 30149
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium sp. IP-I 671
-
purified preparation demonstrates no activity on this substrate
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Serratia liquefaciens 1.15
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens CIP 67.1
-
-
-
-
-
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium rhizogenes 30148
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens 30150
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Geobacillus stearothermophilus JC2310
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Corynebacterium pseudodiphtheriticum 14.10
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens 47C
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Bacillus sp. LU 1220
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
Agrobacterium tumefaciens 30147
-
-
-
-
?
dihydrothymidine + H2O
?
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
r
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
ir
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
r
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Peptococcus anaerobius
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium sp. 30201
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Arthrobacter sp. DSM3747
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Arthrobacter sp. DSM3747
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium rubi 30149
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium sp. IP-I 671
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Serratia liquefaciens 1.15
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Pseudomonas sp. AJ-11220
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium rhizogenes 30148
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium tumefaciens 30150
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus SD-1
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus stearothermophilus JC2310
-
-
-
-
-
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Corynebacterium pseudodiphtheriticum 14.10
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Geobacillus thermocatenulatus GH-2
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium sp. 1-671
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Flavobacterium sp. AJ-3912
-
-
-
-
?
hydantoin + H2O
N-carbamoyl glycine
show the reaction diagram
Agrobacterium tumefaciens 30147
-
-
-
-
?
additional information
?
-
Pseudomonas putida, Pseudomonas putida YZ-26
-
catalyzes the hydrolysis of 5-monosubstituted hydantoin to enantionmeric N-carbamoyl-amino acids
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Al3+
-
at 2 mM 112% relative enzyme activity
Ca2+
-
at 2 mM 123% relative enzyme activity
Co2+
-
stimulates production of L-methionine
Co2+
-
enhances activity by more than 60%
Co2+
-
stimulates
Co2+
-, Q56S49
high stimulation, stimulates enzyme activity most effectively
Co2+
Q84FR6
increases activity, when DL-benzyl-hydantoin is used as substrate
Co2+
-
at 2 mM 162% relative enzyme activity
Co2+
-
79% increase of activity at 1 mM
Co2+
-
2 mM, 331% of initial activity
Cu2+
-, Q56S49
low stimulation
Cu2+
-
at 2 mM 103% relative enzyme activity
Cu2+
-
2 mM, 150% of initial activity
Fe2+
-
needs ferrous ions for activity or reactivation, pure enzyme up to 5 times more active in presence of Fe2+
Fe2+
-
stimulates
Hg2+
-
at 2 mM 0% relative enzyme activity
Mg2+
-
marked acceleration of carbamyl beta-alanine formation from hydrouracil, only slightly stimulatory with hydrothymine as substrate, no effect with hydantoin as substrate
Mg2+
-
at 2 mM 116% relative enzyme activity
Mg2+
-
20% increase of activity at 1 mM
Mg2+
-
2 mM, 121% of initial activity
Mn2+
-
marked acceleration of carbamoyl beta-alanine formation from hydrouracil
Mn2+
-
stimulates production of L-methionine
Mn2+
-
requires 1 ion per molecule of enzyme for activity
Mn2+
-
-
Mn2+
-
depend on
Mn2+
-
stimulates
Mn2+
-
stimulates
Mn2+
-, Q56S49
high stimulation
Mn2+
-
at 2 mM 116% relative enzyme activity, two manganese ions modelled in the active site
Mn2+
-
96% residual activity at 1 mM; compared to wild-type without metal ions, 1 mM Mn2+ enhances the activity up to 200% for wild-type enzyme P479 and to 400500% for truncates mutants P477, P476, P475 and mutant R479D
Mn2+
-
the hydrolytic activity of PydB is enhanced notably by 0.5 mM Mn2+, about an 8fold increase in the enzymatic activity is observed in the presence of 0.05-5 mM Mn2+
Mn2+
-
74% increase of activity at 1 mM
Ni2+
-
stimulates
Ni2+
-
stimulates
Ni2+
-
at 2 mM 124% relative enzyme activity
Ni2+
-
54% increase of activity at 1 mM
Ni2+
-
2 mM, 346% of initial activity
Zinc
-
a binuclear zinc center activates a water molecule for nucleophilic attack on the substrates amide bond
Zinc
-
in vitro preparation of apo-HDT, i.e. metal-removed HDT, and Zn2+-HDT, i.e. Zn2+-added HDT. The Zn2+-HDT and re-HDT contain 2.17 and 0.95 mol Zn2+ per mol subunit, respectively, and have comparable enzymatic activities. In contrast, the apo-HDT only retains 0.04 mol Zn2+ per mol subunit with less than 10% activity, compared with the re-HDT. When the apo-HDT is reconstituted with ZnCl2, the enzymatic activity recovery is about 75%. Data suggest that the re-HDT may have two Zn2+-binding sites, one is an intrinsic or tight-binding site, zinc-alpha, essential for its activity and the other is a vacant or loose-binding site, zinc-beta, possibly non-essential for the activity
Zn2+
-
essential, metalloenzyme
Zn2+
-
essential, metalloenzyme
Zn2+
-
-
Zn2+
-
stimulates
Zn2+
-, Q56S49
low stimulation
Zn2+
Q84FR6
increases activity, when DL-benzyl-hydantoin is used as substrate
Zn2+
-
catalytic di-zinc center embedded by a (beta/alpha)8-barrel structural core, accompanied by a smaller beta-sandwich domain; contains a di-zinc center
Zn2+
-
at 2 mM 118% relative enzyme activity
Zn2+
-
compared to wild-type without metal ions, 1 mM Zn2+ attenuates the enzymatic activity down to 60% for wild-type enzyme P479 and to more than 90% for mutants R479D and truncated mutants P477, P476 and P475; Zn2+-binding enzyme
Zn2+
-
the presence of Zn2+ does not only increase the percentage of secondary structure, but also the thermal and chemical stability of SmelDhp
Zn2+
-
2 mM, 432% of initial activity
Mn2+
-
2 mM, 407% of initial activity
additional information
-, Q56S49
no stimulation with Mg2+, Ni2+, Ca2+ and Fe2+
additional information
Q84FR6
no increase of activity by Fe2+, Mg2+ and Mn2+
additional information
-
non metal ions, 100% relative enzyme activity
additional information
-
binding sites of Zn2+ and Mn2+ may locate at different regions on the enzyme
additional information
-
other divalent metal ions except Mn2+ and Zn2+ do not have significant effect on the enzyme activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,6-dipicolinic acid
-
-
2,6-dipicolinic acid
-
-
4-nitrobenzene sulfonamide
-
-
4-ureidobutyric acid
-
-
5-amino-n-valeric acid
-
-
8-hydroxyquinoline
-
-
8-hydroxyquinoline
-
-
8-Hydroxyquinoline-5-sulfonic acid
-
-
8-Hydroxyquinoline-5-sulfonic acid
-
-
8-Hydroxyquinoline-5-sulfonic acid
-
-
8-Hydroxyquinoline-5-sulfonic acid
-
-
beta-Alanine
-
-
Ca2+
-
97% residual activity at 1 mM
Cd2+
-
44% residual activity at 1 mM
Chloramphenicol
-
10.5fold reduced activity in chloramphenicol-treated cells
Cu2+
-
CuSO4*5H2O strongly inhibits L-methionine production
Cu2+
-
0.4% residual activity at 1 mM
Diethylpyrocarbonate
-
at 2 mM 95% and at 5 mM 80% residual enzyme acitvity
dimethylhydantoin
-
competitive inhibition
EDTA
-
at 5 mM 81% and at 10 mM 71% residual enzyme acitvity
EDTA
-
10 mM, 90% residual activity
glutaric acid monoamide
-
-
Hg2+
-
2 mM, 0% residual activity
L-dihydroorotic acid
-
competitive inhibition
Mercuric chloride
-
1 mM completely abrogates activity
N-bromosuccinimide
-
1 mM completely abrogates activity
N-Carbamoyl-beta-alanine
-
-
N-Carbamoyl-beta-alanine
-
product inhibition
N-carbamoyl-beta-aminoisobutyric acid
-
product inhibition
N-Carbamoyl-glycine
-
competitive inhibition
Nitrilotriacetic acid
-
inhibition 42%
o-Phenathroline
-
at 1 mM 47% and at 2 mM 46% residual enzyme acitvity
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuric benzoate
-
1 mM completely abrogates activity
Propionic acid
-
-
propionic acid amide
-
-
rac-5-methyl-5-phenylhydantoic acid
-
competitive
trans-1,2-cyclohexandiaminetetraacetic acid
-
inhibition 42%
Zn2+
-
irreversible inhibition
Zn2+
-
ZnSO4*7H2O strongly inhibits L-methionine production
Zn2+
-
60% increase of activity at 1 mM
Zn2+
-
a loss of 50% activity is observed in the presence of Zn2+
Zn2+
-
68% residual activity at 1 mM, the dissociation of purified enzyme into its subunits occurrs in the presence of 1 mM Zn2+
Mg2+
-
slightly inhibitory with carbamoyl beta-alanine or carbamoyl beta-aminoisobutyric acid as substrates
additional information
-
not inhibited by N-ethylmaleimide, iodoacetamide, iodoacetic acid, PMSF, phenylglyoxal
-
additional information
-
the use of high sugar to nitrogen ratios in the growth medium results in the formation of extracellular polymers, low biomass, and low enzyme activity
-
additional information
-
not inhibitory: iodoacetamide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
10 mM, 108% of initial activity
2-Thiouracil
-
induces biosynthesis of enzyme
dithiothreitol
-
10 mM, 111% of initial activity
Uracil
-
induces biosynthesis of enzyme
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.017
-
5,6-Dihydrothymine
-
pH 8.2
0.026
-
5,6-Dihydrothymine
-
pH 7.0
0.008
-
5,6-Dihydrouracil
-
-
0.011
-
5,6-Dihydrouracil
-
-
0.019
-
5,6-Dihydrouracil
-
pH 7.0
0.02
-
5,6-Dihydrouracil
-
pH 8.2
1.7
-
5,6-Dihydrouracil
-
-
11
-
5,6-Dihydrouracil
-
-
117
-
5,6-Dihydrouracil
-
-
0.017
-
5-bromo-5,6-dihydrouracil
-
pH 8.2
0.025
-
5-bromo-5,6-dihydrouracil
-
pH 7.0
0.02
-
5-bromodihydrouracil
-
-
0.018
-
5-iododihydrouracil
-
-
34
-
5-isopropylhydantoin
-
-
62.5
-
5-methylhydantoin
-
-
5.8
-
allantoin
-
-
0.028
-
beta-Ureidoisobutyrate
-
0.1 mM
0.069
-
beta-Ureidoisobutyrate
-
0.1-3.25 mM
0.015
-
beta-ureidopropionate
-
0.1 mM
0.079
-
beta-ureidopropionate
-
0.1-5 mM
14.98
-
D,L-benzylhydantoin
-
free enzyme, at pH 8.5 and 45C
27.03
-
D,L-benzylhydantoin
-
immobilized enzyme, at pH 8.5 and 45C
5
-
D-5-benzylhydantoin
-
pH 8.0, 40C
14
-
D-5-butylhydantoin
-
pH 8.0, 40C
-
3.4
-
D-5-ethylhydantoin
-
at 20C
5.1
-
D-5-ethylhydantoin
-
at 25C
7.9
-
D-5-ethylhydantoin
-
at 30C
8.2
-
D-5-ethylhydantoin
-
at 35C
11.6
-
D-5-ethylhydantoin
-
at 40C
18
-
D-5-ethylhydantoin
-
pH 8.0, 40C
10
-
D-5-isobutylhydantoin
-
pH 8.0, 40C
44
-
D-5-isopropylhydantoin
-
pH 8.0, 40C
66
-
D-5-methyl-thio-ethylhydantoin
-
pH 8.0, 40C
72
-
D-5-methylhydantoin
-
pH 8.0, 40C
0.4
-
D-5-methylthioethylhydantoin
-
at 20C
1.4
-
D-5-methylthioethylhydantoin
-
at 25C
2.5
-
D-5-methylthioethylhydantoin
-
at 30C
5.5
-
D-5-methylthioethylhydantoin
-
at 35C
7
-
D-5-methylthioethylhydantoin
-
at 40C
26
-
D-5-propylhydantoin
-
pH 8.0, 40C
-
0.015
-
Dihydrothymine
-
-
2.4
-
dihydrouracil
-, Q56S49
-
3
-
dihydrouracil
-
pH 8.0, 40C
28
-
DL-5-(2-methylthioethyl)-hydantoin
-
-
28
-
DL-5-(2-methylthioethyl)-hydantoin
-
-
34
-
DL-5-(2-methylthioethyl)-hydantoin
-
-
40.7
-
DL-5-(2-methylthioethyl)hydantoin
-
-
20
-
DL-5-(p-hydroxyphenyl)hydantoin
-
pH 8.0, 40C
6.8
-
DL-5-benzylhydantoin
-
-
12.8
-
DL-5-butylhydantoin
-
-
4.9
-
DL-5-isobutylhydantoin
-
-
17.2
-
DL-5-isopropylhydantoin
-
-
1
-
DL-5-methyldihydrouracil
-
pH 8.0, 40C
-
27.8
-
DL-5-methylhydantoin
-
-
7.9
-
DL-5-phenylhydantoin
-
-
50
-
DL-5-phenylhydantoin
-
-
58.8
-
DL-5-phenyllhydantoin
-
-
25.6
-
DL-5-propylhydantoin
-
-
81
-
DL-6-isopropyldihydrouracil
-
pH 8.0, 40C
-
111
-
DL-6-methyldihydrouracil
-
pH 8.0, 40C
-
0.074
-
DL-hydroxyphenylhydantoin
-
-
2.2
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159L
3.5
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159I
3.6
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159V
5.4
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, wild-type enzyme
7.4
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159A
13
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme L65F/F159A
24
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme M63F/L65V
27
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159S
2.9
-
DL-p-hydroxyphenylhydantoin
-
mutant M63I/F159S, pH 8.0, 37C
4.9
-
DL-p-hydroxyphenylhydantoin
-
wild-type, pH 8.0, 37C
5.1
-
DL-p-hydroxyphenylhydantoin
-
mutant M63H/F159N, pH 8.0, 37C
18
-
DL-p-hydroxyphenylhydantoin
Q28SA7
pH 8.0, 40C
1.28
-
hydantoin
-
-
5
-
hydantoin
-, Q56S49
-
8
9
hydantoin
-
pH 8.0, 37C, mutant enzyme F159L
50
-
hydantoin
-
-
78
-
hydantoin
-
wild-type, pH 8.0, 37C
98
-
hydantoin
-
pH 8.0, 37C, wild-type enzyme
105.4
-
hydantoin
-
-
125
-
hydantoin
-
pH 8.0, 40C
160
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159V
190
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159I
260
-
hydantoin
-
pH 8.0, 37C, mutant enzyme M63F/L65V
280
-
hydantoin
-
-
350
-
hydantoin
-
-
382
-
hydantoin
-
mutant M63I/F159S, pH 8.0, 37C
400
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159S
416
-
hydantoin
-
mutant M63H/F159N, pH 8.0, 37C
450
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159A
470
-
hydantoin
-
pH 8.0, 37C, mutant enzyme L65F/F159A
830
-
hydantoin
-
-
2.1
-
Hydrothymine
-
-
117.5
-
Hydrouracil
-
-
0.1
-
L-5-ethylhydantoin
-
at 20C
0.23
-
L-5-ethylhydantoin
-
at 25C
0.43
-
L-5-ethylhydantoin
-
at 30C
2.6
-
L-5-ethylhydantoin
-
at 35C
3.7
-
L-5-ethylhydantoin
-
at 40C
0.4
-
L-5-methylthioethylhydantoin
-
at 20C
0.9
-
L-5-methylthioethylhydantoin
-
at 25C
1.2
-
L-5-methylthioethylhydantoin
-
at 30C
2.6
-
L-5-methylthioethylhydantoin
-
at 35C
4.7
-
L-5-methylthioethylhydantoin
-
at 40C
1.92
-
N-Carbamoyl-beta-alanine
-
pH 7.0
2.26
-
N-Carbamoyl-beta-alanine
-
pH 8.2
1.19
-
N-carbamoyl-beta-aminoisobutyric acid
-
pH 7.0
1.59
-
N-carbamoyl-beta-aminoisobutyric acid
-
pH 8.2
30.7
-
p-hydroxyphenylhydantoin
-
-
47.2
-
p-hydroxyphenylhydantoin
-
-
32.1
-
phenylhydantoin
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2
-
D-5-benzylhydantoin
-
pH 8.0, 40C
3
-
D-5-butylhydantoin
-
pH 8.0, 40C
-
16.6
-
D-5-ethylhydantoin
-
at 20C
17
-
D-5-ethylhydantoin
-
pH 8.0, 40C
20.5
-
D-5-ethylhydantoin
-
at 25C
24.9
-
D-5-ethylhydantoin
-
at 30C
27.3
-
D-5-ethylhydantoin
-
at 35C
37.3
-
D-5-ethylhydantoin
-
at 40C
2
-
D-5-isobutylhydantoin
-
pH 8.0, 40C
5
-
D-5-isopropylhydantoin
-
pH 8.0, 40C
18
-
D-5-methyl-thio-ethylhydantoin
-
pH 8.0, 40C
197
-
D-5-methylhydantoin
-
pH 8.0, 40C
4.9
-
D-5-methylthioethylhydantoin
-
at 20C
6.4
-
D-5-methylthioethylhydantoin
-
at 25C
12.6
-
D-5-methylthioethylhydantoin
-
at 30C
26
-
D-5-methylthioethylhydantoin
-
at 35C
92.6
-
D-5-methylthioethylhydantoin
-
at 40C
6
-
D-5-propylhydantoin
-
pH 8.0, 40C
-
2
-
dihydrouracil
-
pH 8.0, 40C
6.2
-
dihydrouracil
-, Q56S49
-
1
-
DL-5-(p-hydroxyphenyl)hydantoin
-
pH 8.0, 40C
1
-
DL-5-methyldihydrouracil, DL-6-isopropyldihydrouracil
-
pH 8.0, 40C
-
8
-
DL-6-methyldihydrouracil
-
pH 8.0, 40C
-
11
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159L
18
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, wild-type enzyme
46
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159I
50
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159V
110
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159A
180
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme M63F/L65V
200
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme L65F/F159A
280
-
DL-hydroxyphenylhydantoin
-
pH 8.0, 37C, mutant enzyme F159S
4.6
-
DL-p-hydroxyphenylhydantoin
Q28SA7
pH 8.0, 40C
18
-
DL-p-hydroxyphenylhydantoin
-
wild-type, pH 8.0, 37C
59
-
DL-p-hydroxyphenylhydantoin
-
mutant M63I/F159S, pH 8.0, 37C
62
-
DL-p-hydroxyphenylhydantoin
-
mutant M63H/F159N, pH 8.0, 37C
3.04
-
hydantoin
-, Q56S49
-
8
-
hydantoin
-
mutant M63I/F159S, pH 8.0, 37C
12
-
hydantoin
-
pH 8.0, 40C
21
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159S
42
-
hydantoin
-
pH 8.0, 37C, mutant enzyme M63F/L65V
45
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159A
48
-
hydantoin
-
pH 8.0, 37C, mutant enzyme L65F/F159A
57
-
hydantoin
-
mutant M63H/F159N, pH 8.0, 37C
59
-
hydantoin
-
wild-type, pH 8.0, 37C
97
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159V
270
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159I
280
-
hydantoin
-
pH 8.0, 37C, mutant enzyme F159L
440
-
hydantoin
-
pH 8.0, 37C, wild-type enzyme
450
-
hydantoin
-
-
7
-
Hydrothymine
-
-
71.7
-
Hydrouracil
-
-
0.0055
-
L-5-ethylhydantoin
-
at 20C
0.02
-
L-5-ethylhydantoin
-
at 25C
0.061
-
L-5-ethylhydantoin
-
at 30C
0.22
-
L-5-ethylhydantoin
-
at 35C
0.44
-
L-5-ethylhydantoin
-
at 40C
0.6
-
L-5-methylthioethylhydantoin
-
at 20C
1
-
L-5-methylthioethylhydantoin
-
at 25C
3.9
-
L-5-methylthioethylhydantoin
-
at 30C
7.7
-
L-5-methylthioethylhydantoin
-
at 35C
11.4
-
L-5-methylthioethylhydantoin
-
at 40C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.4
-
D-5-benzylhydantoin
-
pH 8.0, 40C
84705
0.214
-
D-5-butylhydantoin
-
pH 8.0, 40C
0
0.944
-
D-5-ethylhydantoin
-
pH 8.0, 40C
263690
0.2
-
D-5-isobutylhydantoin
-
pH 8.0, 40C
84706
0.114
-
D-5-isopropylhydantoin
-
pH 8.0, 40C
84707
0.273
-
D-5-methyl-thio-ethylhydantoin
-
pH 8.0, 40C
310637
2.736
-
D-5-methylhydantoin
-
pH 8.0, 40C
84708
0.231
-
D-5-propylhydantoin
-
pH 8.0, 40C
0
0.667
-
dihydrouracil
-
pH 8.0, 40C
9847
0.05
-
DL-5-(p-hydroxyphenyl)hydantoin
-
pH 8.0, 40C
20902
1
-
DL-5-methyldihydrouracil
-
pH 8.0, 40C
0
0.012
-
DL-6-isopropyldihydrouracil
-
pH 8.0, 40C
0
0.072
-
DL-6-methyldihydrouracil
-
pH 8.0, 40C
0
0.25
-
DL-p-hydroxyphenylhydantoin
Q28SA7
pH 8.0, 40C
235407
3.63
-
DL-p-hydroxyphenylhydantoin
-
wild-type, pH 8.0, 37C
235407
12.17
-
DL-p-hydroxyphenylhydantoin
-
mutant M63H/F159N, pH 8.0, 37C
235407
20.95
-
DL-p-hydroxyphenylhydantoin
-
mutant M63I/F159S, pH 8.0, 37C
235407
0.022
-
hydantoin
-
mutant M63I/F159S, pH 8.0, 37C
11483
0.096
-
hydantoin
-
pH 8.0, 40C
11483
0.137
-
hydantoin
-
mutant M63H/F159N, pH 8.0, 37C
11483
0.763
-
hydantoin
-
wild-type, pH 8.0, 37C
11483
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.28
-
rac-5-methyl-5-phenylhydantoic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00113
-
-
-
0.005
-
-
strain 30207, isopropylhydantoin as substrate
0.00569
-
-
at 30C
0.006
-
-
strain 30201, isopropylhydantoin as substrate
0.007
-
-
strain 30205, isopropylhydantoin as substrate
0.009
-
-
strain 30199, isopropylhydantoin as substrate
0.01
-
-
strain 50106, isopropylhydantoin as substrate
0.011
-
-
strain 2005, isopropylhydantoin as substrate
0.013
-
-
strain 30150, isopropylhydantoin as substrate
0.014
-
-
strain 50106, incubated with hydantoin, isopropylhydantoin as substrate; strain 50108, isopropylhydantoin as substrate
0.015
-
-
strain 50090, incubated with hydantoin, isopropylhydantoin as substrate
0.016
-
-
strain 11291, isopropylhydantoin as substrate
0.016
-
-
strain 50090, isopropylhydantoin as substrate
0.019
-
-
strain 50154, isopropylhydantoin as substrate
0.021
-
-
strain 30204, isopropylhydantoin as substrate
0.022
-
-
strain 2005, isopropylhydantoin as substrate
0.023
-
-
strain 30147, isopropylhydantoin as substrate
0.033
-
-
strain 30201, dihydrouracil as substrate
0.033
-
-
strain 50108, isopropylhydantoin as substrate
0.034
-
-
strain 291, isopropylhydantoin as substrate
0.038
-
-
strain 84, isopropylhydantoin as substrate
0.049
-
-
strain 549, isopropylhydantoin as substrate
0.051
-
-
strain 291, incubated with hydantoin, isopropylhydantoin as substrate
0.059
-
-
strain 11291, dihydrouracil as substrate
0.07
-
-
substrate D-benzylhydantoin
0.084
-
-
DL-5-p-hydroxyphenylhydantoin conversion
0.085
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-(1-methylpropyl)hydantoin
0.091
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-hydantoin-5-acetic acid
0.094
-
-
strain 11291, incubated with hydantoin
0.1
-
-
10 mM dihydrouracil as substrate
0.112
-
-
strain 30205, dihydrouracil as substrate
0.127
-
-
strain 549, incubated with hydantoin, isopropylhydantoin as substrate
0.128
-
-
strain 30150, dihydrouracil as substrate
0.15
-
-
strain 2005, dihydrouracil as substrate
0.17
-
-, Q56S49
EDTA-dialyzed/Co2+-substituted enzyme incubated with hydantoin as substrate
0.185
-
-
strain 84, incubated with hydantoin, isopropylhydantoin as substrate
0.19
-
-
strain 50154, dihydrouracil as substrate
0.193
-
-
strain 30204, dihydrouracil as substrate
0.194
-
-
strain 50090, dihydrouracil as substrate
0.212
-
-
strain 30147, dihydrouracil as substrate
0.216
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-methylhydantoin
0.225
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-hydroxymethylhydantoin
0.299
-
-
strain 30155, dihydrouracil as substrate
0.3
-
-
DL-phenylhydantoin, 50 mM
0.3
-
-
crude extract, at 25C and pH 7.5
0.31
-
-
strain 50106, dihydrouracil as substrate
0.317
-
-
strain 30208, dihydrouracil as substrate
0.36
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Fe2+ with DL-p-hydroxyphenylhydantoin as substrate
0.37
-
-
substrate L-benzylhydantoin
0.377
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by hydantoin
0.385
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-p-hydroxyphenylhydantoin
0.4
-
-
10 mM dihydrothymine as substrate
0.425
-
-
strain 30207, dihydrouracil as substrate
0.46
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Ca2+ with DL-p-hydroxyphenylhydantoin as substrate
0.49
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Ni2+ with DL-p-hydroxyphenylhydantoin as substrate
0.5
-
-
strain 291, dihydrouracil as substrate
0.52
-
-, Q56S49
EDTA-dialyzed enzyme with D,L-p-hydroxyphenylhydantoin as substrate
0.53
-
-
strain 50108, dihydrouracil as substrate
0.54
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Mg2+ with DL-p-hydroxyphenylhydantoin as substrate
0.562
-
-
strain 30206, dihydrouracil as substrate
0.6
-
-
DL-methylhydantoin, 57 mM
0.62
-
-
strain 2005, incubated with hydantoin, dihydrouracil as substrate
0.62
-
-, Q56S49
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-5(2-(methylthio)-ethyl)hydantoin as substrate
0.635
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-phenylhydantoin
0.68
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Cu2+ with DL-p-hydroxyphenylhydantoin as substrate
0.73
-
-
strain 549, incubated with hydantoin, dihydrouracil as substrate
0.73
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Zn2+ with DL-p-hydroxyphenylhydantoin as substrate
0.78
-
-
dihydrouracil as substrate
0.81
-
-
strain 50154, incubated with hydantoin, dihydrouracil as substrate
0.826
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-phenylpropylhydantoin
0.89
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-methylthiethylhydantoin
0.9
-
Q6TTR0
crude extract
0.98
-
-
strain 50108, incubated with hydantoin, dihydrouracil as substrate
1.06
-
-
-
1.11
-
-
strain 50106, incubated with hydantoin, dihydrouracil as substrate
1.128
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-isobutylhydantoin
1.14
-
-
strain 30155, dihydrouracil as substrate
1.2
-
-
strain 549, dihydrouracil as substrate
1.286
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-benylhydantoin
1.4
-
-
strain 50090, incubated with hydantoin, dihydrouracil as substrate
1.44
-
-
DL-hydantoin as substrate
1.5
-
-
L-methylthioethylhydantoin, 2 mM
1.591
-
-
DL-5-p-hydroxyphenylhydantoin conversion induced by DL-5-(2-indolymethyl)hydantoin
1.67
-
-
DL-5-methylhydantoin as substrate
1.69
-
-, Q56S49
EDTA-dialyzed/Co2+-substituted enzyme incubated with DL-p-hydroxyphenylhydantoin as substrate
1.9
-
-
strain 549, incubated with hydantoin, dihydrouracil as substrate
2.25
-
-
incubated with hydantoin
2.38
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Mn2+ with DL-p-hydroxyphenylhydantoin as substrate
3.1
-
Q6TTR0
after 3.4fold purification
3.56
-
-, Q56S49
EDTA-dialyzed/Co2+-substituted enzyme incubated with dihydrouracil as substrate
3.6
-
-, Q56S49
EDTA-dialyzed enzyme incubated with Co2+ with DL-p-hydroxyphenylhydantoin as substrate
5.4
-
-
D-methylthioethylhydantoin, 2 mM
5.93
-
Q84FR6
purified recombinant enzyme from Escherichia coli JM109pJAVI2
6.6
-
-
mutant P477, free enzyme
8.1
-
-
mutant P476, free enzyme
8.2
-
Q28SA7
40C, pH 8.0
8.41
-
-
mutant R479D, free enzyme; purified mutant enzyme R479D, at 37C
9.32
-
-
mutant P478, ifree enzyme
10.1
-
-
-
10.4
-
-
-
10.65
-
-
after 3.4fold purification, using DL-5-phenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
10.75
-
-
mutant P479, free enzyme; purified wild type enzyme, at 37C
10.9
-
-
isopropylhydantoin as substrate
11.84
-
-
after 3.4fold purification, using DL-p-hydroxyphenylhydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
12
-
-
L-methylthioethylhydantoin, 20 mM
13
18
-
-
13
-
-
DL-5-indolylmethylhydantoin as substrate
14.3
-
-
crude extract, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
27.5
-
-
dihydrouracil as substrate
30
-
-
DL-methylthioethylhydantoin, 40 mM
30.3
-
-
crystals
37
-
-
D-methylthioethylhydantoin, 20 mM
48.5
-
-
after 3.4fold purification, using DL-hydantoin as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
66.2
-
-
-
70
-
-
L-5-indolylmethylhydantoin as substrate
76.6
-
-
DL-fluorobromylhydantoin as substrate
91.6
-
-
hydroxyphenylhydantoin as substrate
101.5
-
-
hydantoin as substrate
103.6
-
-
after 334fold purification, at 25C and pH 7.5
136.8
-
-
-
216
-
-
DL-5-phenylhydantoin as substrate
236.9
-
-
phenylhydantoin as substrate
600.8
-
-
after 3.4fold purification, using 5,6-dihydrouracil as a substrate, in 50 mM Tris-HCl buffer, pH 8.0
613
-
-
mutant P475, free enzyme
additional information
-
-
0.01 U/ml cells, mutant DELTA474-479; 0.06 U/ml cells, mutant R479A; 1.32 U/ml cells, mutant DELTA477-479; 1.33 U/ml cells, mutant DELTA478-479; 1.40 U/ml cells, mutant DELTA475-479; 1.48 U/ml cells, mutant DELTA476-479; 1.73 U/ml cells, mutant R479D; 2.12 U/ml cells, mutant DELTA479; no activity in mutant P474, free enzyme; no activity in mutant R479A, free enzyme; only the last four residues can be deleted without significantly affecting the enzyme activity
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.6
-
Q28SA7
-
8
8.3
-
DL-5-phenylhydantoin or hydantoin as substrate
8
-
-
strain 84
8
-
-, Q56S49
DL-p-hydroxyphenylhydantoin as substrate
8
-
-
mutants
8
-
-
in 50mM Tris-HCl buffer
8.2
-
-
borate buffer, hydantoin as substrate
8.2
-
-
resting cells
8.3
8.5
-
DL-5-(2-methylthioethyl)-hydantoin as substrate
8.5
-
-
hydantoin as substrate
8.5
-
-
free enzyme
8.7
9.7
-
reaction with dihydrouracil
8.8
9
-
dihydrouracil as substrate
9
10
-
immobilised enzyme
9
-
-
dihydrouracil as substrate
9
-
-
immobilized enzyme
9.5
-
-
L-5-methylhydantoin as substrate
10
-
-
purified enzyme
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
9.5
-
in 50mM Tris-HCl buffer
7
8
-
wild-type enzyme, 100% activity
7
9
-, Q56S49
DL-p-hydroxyphenylhydantoin as substrate
8
9.7
-
pH 8.0: about 55% of maximal activity, pH 9.7: about 90% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
38
-
-
free enzyme
40
45
-
strain 2.5
40
-
-
immobilised enzyme
45
55
-
strain 1.2
45
55
-
strain 2.2 and 84
45
-
-
DL-5-phenylhydantoin or hydantoin as substrate
45
-
-
DL-5-phenylhydantoin or hydantoin as substrate
45
-
-
resting cells used
45
-
-
resting cells
45
-
-
immobilized enzyme
50
-
Q28SA7
-
55
-
-
dihydrouracil or DL-5-(2-methylthioethyl)-hydantoin as substrate
55
-
-
purified enzyme
57
-
-
strain 1.9
60
70
-
resting cells
60
-
-
L-5-methylhydantoin as substrate
60
-
-, Q56S49
DL-p-hydroxyphenylhydantoin as substrate
65
-
-
hydantoin as substrate
70
-
-
DL-p-hydroxyphenylhydantoin as substrate
75
-
-
DL-5-isopropylhydantoin as substrate
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
80
-, Q56S49
DL-p-hydroxyphenylhydantoin as substrate
60
-
-
unstable at higher temperature
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Agrobacterium tumefaciens NRRL
-
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens NRRL
-
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens NRRL
-
-
-
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
additional information
-
no detectable activity in thyroid gland, muscle, testis, brain cortex, brain gray matter and white matter
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Lachancea kluyveri (strain ATCC 58438 / CBS 3082 / CCRC 21498 / NBRC 1685 / JCM 7257 / NCYC 543 / NRRL Y-12651)
Lachancea kluyveri (strain ATCC 58438 / CBS 3082 / CCRC 21498 / NBRC 1685 / JCM 7257 / NCYC 543 / NRRL Y-12651)
Lachancea kluyveri (strain ATCC 58438 / CBS 3082 / CCRC 21498 / NBRC 1685 / JCM 7257 / NCYC 543 / NRRL Y-12651)
Rhizobium meliloti
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
49290
-
Q84FR6
calculated from sequence data
49680
-
-
MALDI-MS studies, single charged monomer
49880
-
-
calculated from sequence data
50680
-
-
calculated from sequence
51050
-
-
determined by mass spectrometer
51720
-
-
calculated from amino acid sequence
52000
-
-, Q56S49
SDS-PAGE corresponding well with calculated mass of the affinity-tagged translational product of the dhp gene
52040
-
-
MALDI-TOF mass spectromety
52500
-
-
calculated from sequence of cDNA
53000
-
-
SDS-PAGE
53400
-
Q59699
deduced from sequence of cDNA
54000
-
Q84FR6
purified enzyme
54000
-
-
molecular weight of the monomer enzyme and its mutants evaluated by SDS-PAGE, molecular weight of their native forms are determined by gel filtration; SDS-PAGE
54000
-
-
SDS-PAGE
55000
-
Q84FR6
recombinant enzyme, SDS-PAGE
55000
-
Q59699
SDS-PAGE
60000
-
-
SDS-PAGE, corresponding to subunit of the enzyme
100000
-
-
gel filtration
102100
-
-
mass spectrometric analysis
103000
-
-
SDS-PAGE
105000
-
-
gel filtration
108000
-
Q6TTR0
SDS-PAGE
110000
-
-
SDS-PAGE, room temperature, 2-mercaptoethanol
110000
-
-
dynamic light scattering analysis
110000
-
-
SDS-PAGE
113000
-
-
native protein, calculated from hydrodynamic radius
120000
-
-
strain SD-1
133900
-
-
gel filtration
140000
-
-
-
155000
200000
-
-
160000
-
-
gel filtration
190000
-
-
gel filtration
190000
-
-
; strain 2.2, gel filtration
200000
-
-
gel filtration
200000
-
-
native enzyme
200000
-
-
strain NS1122A
200000
-
Q0PQZ5
gel filtration
205000
-
-
strain 2.5, gel filtration
207000
-
-
gel filtration
210000
-
-
-
210000
-
-
gel filtration
212000
-
-
gel filtration
215000
-
-
gel filtration
226000
-
-
-
226000
-
-
gel filtration
226000
-
-
-
226000
-
-
gel filtration
230000
-
-
gel filtration
230000
-
-
gel filtration
232000
-
-
strain DSM 84, gel filtration
235000
-
-
strain 1.2 and 1.9, gel filtration
247000
-
-
calculated according to the repective amount of Zn2+
253000
-
Q28SA7
gel filtration
257000
-
-
gel filtration
266000
-
-
-
360000
-
-
artificial fusion enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
A1E351, -
x * 52000, SDS-PAGE
dimer
-
2 * 51100, mass spectrometric analysis; 2 * 54000, SDS-PAGE
dimer
-
-
dimer
-
2 * 50000, SDS-PAGE
dimer
-
2 * 52000, reducing and nonreducing SDS-PAGE; 2 * 52253, mass spectrometric analysis
dimer
-
; wild-type P479, determined by gel filtration
dimer
Q6TTR0
2 * 54000, SDS-PAGE
dimer
Geobacillus stearothermophilus SD-1
-
; 2 * 51100, mass spectrometric analysis; 2 * 54000, SDS-PAGE
-
dimer
Pseudomonas putida YZ-26
-
; 2 * 54000, SDS-PAGE; wild-type P479, determined by gel filtration
-
homodimer
-
2 * 54000, SDS-PAGE
homodimer
-
2 * 52000, SDS-PAGE
homodimer
Pseudomonas putida YZ-26
-
2 * 52000, SDS-PAGE; 2 * 54000, SDS-PAGE
-
homotetramer
-
4 * 62300, His8-tagged protein
homotetramer
-
structure solved by molecular replacement, corresponding to the native state of the enzyme in solution
homotetramer
-
4 * 52000
homotetramer
Burkholderia cepacia njut01
-
4 * 52000
-
monomer
-
recombinant enzyme expressed in Escherichia coli
monomer
-
mutant enzymes R479D, P478, P477, P476, P475, and P474, determined by size-exclusion chromatography
monomer
-
recombinant enzyme expressed in Escherichia coli
-
monomer
Pseudomonas putida YZ-26
-
; mutant enzymes R479D, P478, P477, P476, P475, and P474, determined by size-exclusion chromatography
-
oligomer
-
2 * 60000 + 2 * 62000 + 1 * 110000, room temperature, 2-mercaptoethanol, SDS-PAGE
tetramer
-
4 * 60000, SDS-PAGE
tetramer
-
4 * 56500, SDS-PAGE
tetramer
-
4 * 54000, SDS-PAGE
tetramer
-
4 * 54000, SDS-PAGE
tetramer
-
4 * 55000-62000
tetramer
-
4 * 55000, SDS-PAGE
tetramer
-
4 * 52000, recombinant enzyme, SDS-PAGE
tetramer
-
4 * 53000, SDS-PAGE; 4 * 54000, gel filtration, denaturing conditions
tetramer
-
4 * 49670
tetramer
-
-
tetramer
-
4 * 56000, SDS-PAGE
tetramer
-
4 * 60000, SDS-PAGE
tetramer
-
4 * 86000, artificial fusion protein, SDS-PAGE
tetramer
-
each subunit of the tetrameric enzyme consists of an elliptically distorted (alpha/beta)8-barrel domain
tetramer
-
; x-ray crystallography
tetramer
-
x-ray crystallography
tetramer
-
purified enzyme from the native Bacillus sp. AR9 by gel filtration
tetramer
Q0PQZ5
x-ray crystallography
tetramer
-
in the absence of Zn2+, the protein acquires a tetrameric functional structure at pH 6.0, which is stable up to pH 9
tetramer
Q28SA7
4 * 56000, His-tagged recombinant protein
tetramer
Arthrobacter sp. DSM3747
-
; 4 * 55000-62000
-
tetramer
-
purified enzyme from the native Bacillus sp. AR9 by gel filtration
-
tetramer
Geobacillus stearothermophilus JC2310
-
-
-
tetramer
Geobacillus stearothermophilus NS1122A
-
4 * 55000, SDS-PAGE
-
tetramer
Geobacillus stearothermophilus SD-1
-
; 4 * 86000, artificial fusion protein, SDS-PAGE
-
tetramer
Geobacillus thermocatenulatus GH-2
-
4 * 56000, SDS-PAGE; 4 * 86000, artificial fusion protein, SDS-PAGE
-
tetramer
Sinorhizobium meliloti CECT4114
-
x-ray crystallography
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2.6 A resolution. Crystals belong to the space group p2(1) with unit cell parameters a = 111.55 A, b = 74.28 A, c = 146.87 A and beta = 106.7 A
-
hanging-drop vapour-diffusion method, crystals belong to the space group P6(4) and have unit-cell parameters a = b = 129.55, c = 102.86 A, alpha = beta = 90, gamma = 120
-
by vapour-diffusion method with sitting drops
-
with 100 mM Bistris, pH 6.5, 20% (w/v) PEG 3350, and 100 mM trisodium citrate; X-ray structure at at 2.05 A resolution
-
docking of the productive transition states of hydantoin, DL-p-hydroxyphenylhydantoin, 1-p-hydroxyphenylhydantoin and dihydrouracil into the active site to simulate the mode of substrate binding. The substrate binding pocket accommodates substrates with small side chains better than those with large ones. The bulky side chains of Phe63, Leu92, Phe150 and Tyr153 are in close contact with the exocyclic substituent of D-p-hydroxyphenylhydantoin
Q28SA7
equilibration, at 2.4 A resolution; with 100 mM Bistris, pH 6.5, 20% (w/v) PEG 3350, and 100 mM trisodium citrate
-
hangin-drop vapour-diffusion method
-
hanging-drop vapour-diffusion method, crystal structure at 2.7 A resolution
-
hanging drop vapour diffusion method with 4.0 M sodium formate, 0.1 M sodium acetate pH 4.6 as precipitant in the presence of agarose at 0.1% (w/v)
Q0PQZ5
in the absence and in the presence of zinc, hanging drop vapour diffusion method
-
to 1.85 A resolution. Enzyme consists of two domains, an (alpha/beta)8 TIM-like barrel domain, where the catalytic centre is located, and a smaller beta-sheet sandwich domain of unknown function. The C-terminal tails of each subunit extend toward another monomer in a swapping-like manner, creating a hydrogen bond network which suggests its implication in protein oligomerisation. Mutational and structural evidence suggest the involvement of a conserved tyrosine in the reaction mechanism of the enzyme
-
hanging-drop vapour-diffusion method. Two forms of crystals are grown under quite different crystallization conditions: needle-shaped form A crystals diffract to 2.2 A and belong to the triclinic space group with unit cell parameters a = 96.35 A, b = 96.87 A, c = 154.87 A, alpha = 82.1, beta = 72.54 and gamma = 77.19. Form B crystals diffract to 3.0 A and belong to the orthorhombic space group C222(1), with unit cell parameters A = 113.92 A, b = 157.22 A and c = 156.21 A
-
crystals belong to orthorhombic space group C222
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
7
-
strain 2.2, other strains more stable around pH 5
5
-
-
strain 84
5.5
8.5
-
most stable in this range, more stable at alkaline pH than at acid pH
5.5
-
-
remains stable at pH values higher than
5.5
-
-
the enzyme activity is completely lost below pH 5.5
6
12
-
the enzymatic activity is completely lost under pH 6.0, it maintains nearly 60% activity at a pH above 12
6
7
-
most stable in this range, more stable at alkaline than at acidic pH
6
9
-
in the absence of Zn2+, the protein acquires a tetrameric functional structure at pH 6.0, which is stable up to pH 9
6
9.5
-
enzyme inactivated at pH 3.5-4 and above pH 10.5
7
10
Q6TTR0
-
7.1
7.2
-
more stable in slightly alkaline buffers
8.5
9.5
-
most stable in this range
9
-
-
immobilized enzyme is completely stable
additional information
-
-
immobilized enzyme is significantly inactivated at pH 10 and pH 8.0
additional information
-
-
pH stability of mutant enzymes R479D and C-terminally truncated mutants P477, P476, and P475 is significantly higher than that of the wild-type enzyme at both acidic and basic sides.; The influence of pH, either on the wild-type enzyme or on mutants, is close to the optimal pH from pH 8.0 to 9.0; The relative activity of mutants is about twice as that of the wild-type enzyme at pH 6.0 and pH 10.0
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
25
-
the relative activity of immobilized D-hydantoinase is dramatically enhanced when the temperature increased from 5 to 25C
30
-
-
strain 2.2 and 84, stable activity for at least 50 h
30
-
-
30 min, about 75% of the activity that is retained after treatment for 30 min at 4C
37
-
-
stable up to at pH 5
37
-
-
strain 2.5
37
-
-
stable up to at pH 5
40
80
-
fairly thermostable, oligomer retains full activity at 40-60C, still retains 98% initial activity at 70C
40
-
-
stable at temperatures up to, starts to lose significant activity at 50C, remaining activity at 55C less than 25%, heat treatment in presence of substrate stabilizes enzyme at temperatures in excess of 40C
40
-
-
30 min, about 65% of the activity that is retained after treatment for 30 min at 4C
40
-
-
30 min, stable
45
-
-
strains 1.2 and 1.9
45
-
-
stable up to
50
55
-
only stable at a temperature below 50C, activity is rapidly lost over 55C
50
90
-
the enzyme is completely thermostable at 50C for 20 days, and 50% of its initial activity remains after 36 days of incubation. After that, enzymatic activity gradually decreases to 4.4% after 65 days. Less than 15% maximum activity is observed at 90C for 10 min.
50
-
-
50% initial activity remains after 6 h, activity decreases within min by incubating at 60C
50
-
-
highly denatured at temperatures above
50
-
-
30 min, about 45% of the activity that is retained after treatment for 30 min at 4C
50
-
-
30 min, 11% loss of activity in absence of metal ions or in presence of Co2+ or Mn2+
51
-
-
more than 40% activity lost after 10 min
57
-
-
stable up to at above pH 8
60
-
-
stable up to, readily loses activity above 65C
60
-
-
stable up to, retains full activity on heating at 40C for 8 h, retains 75% original activity at 62.5C for 8 h
60
-
Peptococcus anaerobius
-
half-life of 1 h
60
-
-
30 min, about 35% of the activity that is retained after treatmet for 30 min at 4C
60
-
-
30 min, 65% loss of activity in absence of metal ions, 20% loss of activity in presence of Co2+ or Mn2+
60
-
-
at 60C, the immobilized D-hydantoinase remains 90% activity at least, but the immobilized enzyme form decreases sharply to 65% of the activity
60
-
-
6 h, 95% residual activity
70
-
-
treatment for 30 min, 20% activity remains
70
-
-
retains 30% of its activity in 30 min
70
-
-
half-life of 80 min, loses only 33% activity in 4 h at 60C, retains 90% activity in the range 55-70C, half-life of more than 12 h at 50C, retains more than 70% activity at 60C
70
-
-
30 min, about 20% of the activity that is retained after treatment for 30 min at 4C
70
-
-
30 min, complete loss of activity above, in absence of metal ions. 85% loss of activity in presence of Co2+ or Mn2+
70
-
-
free enzyme loses 60% of activity in 10 min, immobilized enzyme retains full activity
70
-
-
30 min, 25% residual activity
80
-
-
half-life 30 min
80
-
-
half-life about 45 min, stable up to 75C
80
-
-
half-life of 20 min
80
-
-
30 min, about 15% of the activity that is retained after treatment for 30 min at 4C
additional information
-
-
The thermal stability of mutant entyme P479 is higher than that of all mutants under the same conditions. The oligomeric D-hydantoinase may be less flexible and resist the thermal inactivation. The monomer enzyme has less these interaction forces that may affect its stability under the externally environmental factors.
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
freezing of the enzyme lead to its subsequent inactivation
-
reactions catalyzed by both free and immobilized D-hydantoinase are accelerated under microwave irradiation
-
the half-time of the immobilized D-hydantoinase (onto polystyrene anion exchange resin via glutaraldehyde) is 11 days
-
fairly stable during storage in appropriate buffers
-
after immobilisation on Eupergit C, the hydantoinase activity is retained at 86% of the unimmobilised level and 15% of this activity is retained even after 4 weeks, as compared with the unimmobilised hydantoinase activity which is completely lost after 2 weeks
-
the presence of Zn2+ does not only increase the percentage of secondary structure, but also the thermal and chemical stability of Dhp
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-30C, Luria-Bertani medium, 40% (v/V) glycerol
-
-20C, lactate medium
Q84FR6
5C, phosphate buffer, can be stored under weakly alkaline conditions for at least several weeks,
-
-20C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, 50% initial activity left after 6 months
-
4C, 5 mM Tris buffer pH 8.5, 0.05 mM MnCl2, retains full activity 1 month
-
50C, 10 days
-, Q56S49
-20C, stable for several months
-
4C, dialyzed against phosphate or veronal buffer, pH 6.9 and pH 8.6, without loss of activity
-
4C, stable in dilute Tris buffer pH 7.5 for several days with no loss of activity, purified enzyme loses up to 50% activity after 6 months
-
4C, onto polystyrene anion exchange resin via glutaraldehyde-immobilized D-hydantoinase, 100 days, nearly 90% of activity is reserved
-
-80C, phosphate buffer, pH 7.0, 10% (v/v) glycerol
-
-20C, HEPES buffer, pH 8.0, 3% glycerol
-
-20C, Tris buffer, pH 7.5, shorter times
-
-80C, Tris buffer, pH 7.5, longer times
-
4C, potassium phosphate buffer at pH 8 permits best enzyme recovery and assured stability of enzyme for more than 14 days
-
-20C, stable for protracted periods
-
-70C, no loss of activity for several months
-
4C, less than 20% activity lost after 2-3 days
-
4C, purified enzyme, 20% decrease in specific activity observed within 2 months
-
-5C, H3BO3/KCl buffer, pH 9.0
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
6-His-tagged enzyme was purified to homgeneity by His-Bind resin affinity chromatography
-
recombinant enzyme from Escherichia coli, purification in one chromatographic step without affinity tag
-
TALON metal affinity resin column chromatography
-
using artificial oil bodies
-
enzyme from wild type 19.8fold purified after MonoQ chromatography, recombinant enzyme from Escherichia coli JM109pJAVI2 90% purified by Strep-tag technology
Q84FR6
by immobilized metal affinity chromatography
-, Q56S49
Ni-NTA column chromatography
-
Phenyl Sepharose Fast Flow column chromatography
-
gel filtration, metal-affinity chromatography
-
artificial fusion enzyme
-
recombinant enzyme
-
artificial fusion enzyme
-
recombinant protein
Q28SA7
gel filtration
-
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration
Q6TTR0
by ammonium sulfate fractionation, Phenyl-Sepharose hydrophobic interaction chromatography and Sephacryl S-200 gel filtration
-
purification of mutants by a two-step chromatography, an ion exchange chromatography following by a hydrophobic chromatography; Q-Sepharose column chromatography, ammonium sulfate precipitation, and phenyl-Sepharose column chromatography; Q-Sepharose Fast Flow chromatography and Phenyl-Sepharose Fast Flow column chromatography
-
Talon metalaffinity resin column chromatography and Superdex 200 gel filtration
Q0PQZ5
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells in insoluble form
-
expression in Escherichia coli NovaBlue
-
expression in Escherichia coli
-
expression in Escherichia coli JM109
Q84FR6
expression in Escherichia coli
A1E351, -
expression in Escherichia coli JM109
-
overexpression of His6-tagged DHP in Escherichia coli Novablue cells
-, Q56S49
expressed in Escherichia coli strain JM109, the expression level of dihydropyrimidinase in the recombinant Escherichia coli cells is estimated to be about 20% of the respective total soluble proteins
-
expression in Escherichia coli
-
overexpression as His8-tagged fusion protein in Escherichia coli BL21
-
gene for thermostable hydantoinase cloned and overexpressed in Escherichia coli JM105, vector pAHN134
-
strain SD1, D-hydantoinase gene cloned and sequenced
-
gene cloned and sequenced, identical with B. stearothermophilus NS1122A gene
-
cDNA sequences of dihydropyrimidinase determined
-
expressed in Escherichia coli
-
expression in Escherichia coli, His-tag
Q28SA7
expression as His8-tagged protein in Escherichia coli BL21
-
expression in Escherichia coli JM109
-
expression in Escherichia coli
-
a series of mutants of the enzyme with the C-terminal residues either deleted or substituted are prepared, expression in Escherichia coli; expressed in Escherichia coli BL21 (DE3) cells
-
D-hydantoinase gene cloned, sequenced and expressed in Escherichia coli
-
expressed by pET-HDT/Escherichia coli BL21
-
expressed in Escherichia coli BL21 (DE3) cells
Q6TTR0
expressed in Escherichia coli strain DG116
-
expressed in Escherichia coli strain pE-HDT/BL21
-
expressed in Escherichia coli strains strains BL21 DE3 and JM109 (as a His-tagged enzyme)
Q59699
expression in Escherichia coli
-
DL-hydantoinase cloned from 172-kb native plasmid
-
expression in Escherichia coli
-
cDNA sequences of dihydropyrimidinase determined
-
expressed in Escherichia coli BL21 cells
Q0PQZ5
D-hydantoinase genes cloned and sequenced, Escherichia coli transformed with cosmid vectors, D-hydantoinase expressed and identified; expressed in Escherichia coli BL21
-
expressed in Escherichia coli B834; expressed in Escherichia coli BL21
-
expressed in Escherichia coli BL21
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
significant reduction in both hydantoinase and N-carbamoylase activities in cells grown in medium containing the repressive nitrogen source 0.5% casamino acids as compared with the non-repressive nitrogen source 0.02% casamino acids
-
hydantoinase and N-carbamoylase activities are regulated by the growth phase of the cell, the presence or absence of hydantoin, and these regulatory pathways act at both the transcriptional and post-transcriptional levels. hyuH transcription is not sensitive to nitrogen catabolite repression in strain RU-AE01
-
hydantoinase and N-carbamoylase activity are induced approximately 3fold in cells grown in medium with hydantoin as compared with 0.02% casamino acids as a nitrogen source. A 2fold increase in hydantoinase gene hyuH transcriptional activity is observed in medium containing hydantoin as compared with cells grown in minimal medium
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
F159A
-
turnover number for hydantoin is 10% of that of the wild-type enzyme, Km-value for hydantoin is 4.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 6.1fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme
F159I
-
turnover number for hydantoin is 61% of that of the wild-type enzyme, Km-value for hydantoin is 1.9fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 2.6fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.5fold lower than that of the wild-type enzyme
F159L
-
turnover number for hydantoin 64% of the turnover number of the wild-type enzyme, Km-value for hydantoin is 90% of the value of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 61% of that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 2.5fold lower than that of the wild-type enzyme
F159S
-
turnover number for hydantoin is 4.7% of that of the wild-type enzyme, Km-value for hydantoin is 4fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 15.5fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 5fold higher than that of the wild-type enzyme
F159S
-
400% of wild-type activity
F159V
-
turnover number for hydantoin is 22% of that of the wild-type enzyme, Km-value for hydantoin is 1.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 2.8fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.5fold lower than that of the wild-type enzyme
L65F/F159A F159A
-
turnover number for hydantoin is 10.9% of that of the wild-type enzyme, Km-value for hydantoin is 4.8fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 11fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 2.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme
M63A
-
120% of wild-type activity
M63A/F159S
-
411% of wild-type activity
M63F/L65V F159A
-
turnover number for hydantoin is 9.5% of that of the wild-type enzyme, Km-value for hydantoin is 2.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 10fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 4.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme
M63H/F159N
-
450% of wild-type activity
M63H/F159R
-
168% of wild-type activity
M63H/F159S
-
353% of wild-type activity
M63I
-
108% of wild-type activity
M63I/F159A
-
374% of wild-type activity
M63I/F159S
-
540% of wild-type activity
M63I/F159T
-
411% of wild-type activity
M63Q/F159N
-
213% of wild-type activity
F159A
Geobacillus stearothermophilus SD1
-
turnover number for hydantoin is 10% of that of the wild-type enzyme, Km-value for hydantoin is 4.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 6.1fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.4fold higher than that of the wild-type enzyme. Specificity of the mutant enzyme towards aromatic substrate hydroxyphenylhydantoin is enhanced by 200fold compared with that of the wild-type enzyme
-
F159I
Geobacillus stearothermophilus SD1
-
turnover number for hydantoin is 61% of that of the wild-type enzyme, Km-value for hydantoin is 1.9fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 2.6fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.5fold lower than that of the wild-type enzyme
-
F159L
Geobacillus stearothermophilus SD1
-
turnover number for hydantoin 64% of the turnover number of the wild-type enzyme, Km-value for hydantoin is 90% of the value of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 61% of that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 2.5fold lower than that of the wild-type enzyme
-
F159S
Geobacillus stearothermophilus SD1
-
turnover number for hydantoin is 4.7% of that of the wild-type enzyme, Km-value for hydantoin is 4fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 15.5fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 5fold higher than that of the wild-type enzyme
-
F159V
Geobacillus stearothermophilus SD1
-
turnover number for hydantoin is 22% of that of the wild-type enzyme, Km-value for hydantoin is 1.6fold higher than that of the wild-type enzyme, turnover-number for DL-hydroxyphenylhydantoin is 2.8fold higher than that of the wild-type enzyme, Km-value for DL-hydroxyphenylhydantoin is 1.5fold lower than that of the wild-type enzyme
-
R412M
-
inactive
R412M
-
inactive enzyme, missense mutation causing dihydropyrimidase deficiency
W360R
-
inactive
W360R
-
inactive enzyme, missense mutation causing dihydropyrimidase deficiency
F150C
Q28SA7
about 5% of wild-type activity
F150D
Q28SA7
about 5% of wild-type activity
F150E
Q28SA7
about 5% of wild-type activity
F150G
Q28SA7
about 8% of wild-type activity
F150H
Q28SA7
about 5% of wild-type activity
F150I
Q28SA7
about 10% of wild-type activity
F150K
Q28SA7
about 5% of wild-type activity
F150L
Q28SA7
about 10% of wild-type activity
F150M
Q28SA7
about 15% of wild-type activity
F150N
Q28SA7
about 5% of wild-type activity
F150P
Q28SA7
about 5% of wild-type activity
F150Q
Q28SA7
about 5% of wild-type activity
F150R
Q28SA7
about 5% of wild-type activity
F150S
Q28SA7
about 5% of wild-type activity
F150T
Q28SA7
about 5% of wild-type activity
F150V
Q28SA7
about 5% of wild-type activity
F150W
Q28SA7
about 5% of wild-type activity
F150Y
Q28SA7
about 18% of wild-type activity
F63C
Q28SA7
about 75% of wild-type activity
F63D
Q28SA7
about 15% of wild-type activity
F63E
Q28SA7
about 10% of wild-type activity
F63G
Q28SA7
about 50% of wild-type activity
F63H
Q28SA7
about 115% of wild-type activity
F63I
Q28SA7
about 80% of wild-type activity
F63K
Q28SA7
about 35% of wild-type activity
F63L
Q28SA7
about 105% of wild-type activity
F63M
Q28SA7
about 95% of wild-type activity
F63N
Q28SA7
about 110% of wild-type activity
F63P
Q28SA7
about 10% of wild-type activity
F63Q
Q28SA7
about 25% of wild-type activity
F63R
Q28SA7
about 30% of wild-type activity
F63S
Q28SA7
about 75% of wild-type activity
F63T
Q28SA7
about 70% of wild-type activity
F63V
Q28SA7
about 70% of wild-type activity
F63W
Q28SA7
about 95% of wild-type activity
F63Y
Q28SA7
about 125% of wild-type activity
L157A
Q28SA7
33.6% of wild-type activity
L157D
Q28SA7
11% of wild-type activity
L157I
Q28SA7
94.0% of wild-type activity
L157V
Q28SA7
98.3% of wild-type activity
L92C
Q28SA7
about 25% of wild-type activity
L92D
Q28SA7
about 10% of wild-type activity
L92E
Q28SA7
about 10% of wild-type activity
L92F
Q28SA7
about 95% of wild-type activity
L92G
Q28SA7
about 10% of wild-type activity
L92H
Q28SA7
about 15% of wild-type activity
L92I
Q28SA7
about 120% of wild-type activity
L92K
Q28SA7
about 20% of wild-type activity
L92M
Q28SA7
about 115% of wild-type activity
L92N
Q28SA7
about 10% of wild-type activity
L92P
Q28SA7
about 5% of wild-type activity
L92Q
Q28SA7
about 15% of wild-type activity
L92R
Q28SA7
about 5% of wild-type activity
L92S
Q28SA7
about 10% of wild-type activity
L92T
Q28SA7
about 35% of wild-type activity
L92V
Q28SA7
about 70% of wild-type activity
L92W
Q28SA7
about 10% of wild-type activity
L92Y
Q28SA7
about 5% of wild-type activity
DELTA474-479
-
C-terminally truncated mutant, expressed in the form of random aggregates without any activity
DELTA475-479
-
C-terminally truncated mutant, dissociated into the monomeric state, the activity is largely retained
DELTA476-479
-
C-terminally truncated mutant, dissociated into the monomeric state, the activity is largely retained
DELTA477-479
-
C-terminally truncated mutant, dissociated into the monomeric state, the activity is largely retained
R474
-
mutant is expressed in the form of random aggregates without any activity
R479A
-
expressed in the form of random aggregates without any activity; mutant is expressed in the form of random aggregates without any activity; the mutant is expressed in the form of random aggregates without any activity, the relative activity of mutant is about twice as that of the wild type enzyme at pH 6.0 and pH 10.0
R479A
Pseudomonas putida YZ-26
-
expressed in the form of random aggregates without any activity; mutant is expressed in the form of random aggregates without any activity
-
DELTA478-479
-
C-terminally truncated mutant, dissociated into the monomeric state, the activity is largely retained
additional information
-
several truncated mutants (P478, P477, P476, and P475) are dissociated into the monomeric state as well, but their activities are largely retained, the relative activities of mutants is about twice as that of the wild type enzyme at pH 6.0 and pH 10.0; the flexibility of the non-conservative region at the C-terminus is quite limited implying that the intact enzyme structure is essential for enzyme activity; the truncated mutants P478, P477, P476, and P475 are dissociated into the monomeric state, but their activities are largely retained (86.7-57.0% of wild type activity)
additional information
Q6TTR0
HYDc1 with the C-terminal Arg deletion retains 43% activity, while HYDn1 with the N-terminal Ser deletion has no activity using DL-hydantoin as substrate
R479D
-
dissociated into the monomeric state, activity is largely retained; dissociates into the monomeric state but 78.2% retains activity of the wild type enzyme; the C-terminal-substituted enzyme is dissociated into the monomeric state, but the activity is largely retained, the relative activity of mutant is about twice as that of the wild type enzyme at pH 6.0 and pH 10.0
additional information
Pseudomonas putida YZ-26
-
HYDc1 with the C-terminal Arg deletion retains 43% activity, while HYDn1 with the N-terminal Ser deletion has no activity using DL-hydantoin as substrate; several truncated mutants (P478, P477, P476, and P475) are dissociated into the monomeric state as well, but their activities are largely retained, the relative activities of mutants is about twice as that of the wild type enzyme at pH 6.0 and pH 10.0; the flexibility of the non-conservative region at the C-terminus is quite limited implying that the intact enzyme structure is essential for enzyme activity; the truncated mutants P478, P477, P476, and P475 are dissociated into the monomeric state, but their activities are largely retained (86.7-57.0% of wild type activity)
-
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
artificial oil bodies are treated with 0.04 mM of 1,4-dithiothreitol for 16 h and centrifugation is then applied to segregate the oil and aqueous phase containing the refolded enzymes
-
when the apo-HDT depleted of zinc is reconstituted with ZnCl2, the enzymatic activity recovery is about 75%
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides
medicine
-
D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides; development of the enzymatic process for production of D-p-hydroxyphenylglycine from DL-5-hydroxyphenylhydantoin, new drugs based on p-hydroxyphenylglycine like aspoxicillin, cefbuperazine and cepyramide expected to be marketed in near future
medicine
Agrobacterium sp. 1-671
-
D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides; development of the enzymatic process for production of D-p-hydroxyphenylglycine from DL-5-hydroxyphenylhydantoin, new drugs based on p-hydroxyphenylglycine like aspoxicillin, cefbuperazine and cepyramide expected to be marketed in near future
-
medicine
Agrobacterium sp. IP-I 671
-
D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides; D-amino acids for preparation of beta-lactam antibiotics like semisynthetic penicillins and cephalosporins such as ampicillin, amoxicillin and cefadroxyl, peptide hormones and pesticides; development of the enzymatic process for production of D-p-hydroxyphenylglycine from DL-5-hydroxyphenylhydantoin, new drugs based on p-hydroxyphenylglycine like aspoxicillin, cefbuperazine and cepyramide expected to be marketed in near future
-
synthesis
-
; commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
synthesis
-
artificial fusion enzyme useful as potential biocatalyst for production of nonnatural amino acids
synthesis
-
encapsulation of a crude cell extract from Agrobacterium radiobacter containing D-hydantoinase and D-carbamoylase activities into alginate-chitosan polyelectrolyte complexes with negligible leakage from the formed capsules. The most suitable biocatalysts are prepared using a chitosan with a medium molecular weight of 600 kDa and a degree of deacetylation of 0.9. For all of the preparation conditions under study, an encapsulation yield of around 60% was achieved and the enzymatic activity yields ranged from 30 to 80% for D-hydantoinase activity and from 40 to 128% for D-carbamoylase activity relative to the activities of the soluble extract. All of the biocatalysts are able to hydrolyze LD-hydroxyphenylhydantoin into p-hydroxyphenylglycine with yields ranging from 30 to 80%
synthesis
Agrobacterium tumefaciens 30147
-
artificial fusion enzyme useful as potential biocatalyst for production of nonnatural amino acids
-
synthesis
Agrobacterium tumefaciens 47C
-
; commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
-
synthesis
-
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
synthesis
-
production of D-N-carbamoyl amino acid
nutrition
Corynebacterium pseudodiphtheriticum 14.10
-
-
-
synthesis
-
artificial fusion enzyme useful as potential biocatalyst for production of nonnatural amino acids
nutrition
-
no degradative activity for L-tryptophan, method for L-tryptophan production is very promising as a commercial process
medicine
-
industrial production of L-amino acids
synthesis
-
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
synthesis
-
artificial fusion enzyme useful as potential biocatalyst for production of nonnatural amino acids
synthesis
Geobacillus stearothermophilus JC2310
-
commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals
-
synthesis
Geobacillus stearothermophilus SD-1
-
; artificial fusion enzyme useful as potential biocatalyst for production of nonnatural amino acids; commercial production of optically pure amino acids for synthesis