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Literature summary for 3.5.2.2 extracted from

  • Chiang, C.J.; Chen, H.C.; Chao, Y.P.; Tzen, J.T.
    One-step purification of insoluble hydantoinase overproduced in Escherichia coli (2007), Protein Expr. Purif., 52, 14-18.
    View publication on PubMed
Search for more literature for 3.5.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells in insoluble form Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 SDS-PAGE Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens NRRL B-11291
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using artificial oil bodies Agrobacterium tumefaciens

Renatured (Commentary)

Renatured (Comment) Organism
artificial oil bodies are treated with 0.04 mM of 1,4-dithiothreitol for 16 h and centrifugation is then applied to segregate the oil and aqueous phase containing the refolded enzymes Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 crude extract, at 25°C and pH 7.5 Agrobacterium tumefaciens
103.6
-
 after 334fold purification, at 25°C and pH 7.5 Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-hydroxyphenylhydantoin + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
DL-hydroxyphenylhydantoin + H2O
-
 Agrobacterium tumefaciens NRRL B-11291 ?
-
 ?

Synonyms

Synonyms Comment Organism
HDT
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Agrobacterium tumefaciens