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3.5.2.2: dihydropyrimidinase

This is an abbreviated version!
For detailed information about dihydropyrimidinase, go to the full flat file.

Word Map on EC 3.5.2.2

Reaction

5,6-dihydrouracil
+
H2O
=
3-ureidopropanoate

Synonyms

4,5-dihydropyrimidine amidohydrolase, aaHYD, bar9HYD, bpHYD, bsHYD, CRMP, D-CpHPG, D-HYD, D-hydantionase, D-hydantoin-hydrolyzing enzyme, D-hydantoinase, D-PfHYD, DHP, DHP-1, DHP-2, DHPase, dht, DHTase, dihydropyrimidase/hydantoinase, Dihydropyrimidinase, dihydropyrimidine amidohydrolase, dihydropyrimidine dehydrogenase, HDT, HYD, hydantoin peptidase, hydantoinase, hydropyrimidine hydrase, hyuH, L-Hyd, L-hydantoinase, microbial hydantoinase, P479, PA0441, PaDHPase, PYD2, PydB, pyrimidine hydrase, SmelDhp, thHYD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.2 dihydropyrimidinase

General Stability

General Stability on EC 3.5.2.2 - dihydropyrimidinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after immobilisation on Eupergit C, the hydantoinase activity is retained at 86% of the unimmobilised level and 15% of this activity is retained even after 4 weeks, as compared with the unimmobilised hydantoinase activity which is completely lost after 2 weeks
-
fairly stable during storage in appropriate buffers
-
freezing of the enzyme lead to its subsequent inactivation
-
reactions catalyzed by both free and immobilized D-hydantoinase are accelerated under microwave irradiation
-
the half-time of the immobilized D-hydantoinase (onto polystyrene anion exchange resin via glutaraldehyde) is 11 days
-
the presence of Zn2+ does not only increase the percentage of secondary structure, but also the thermal and chemical stability of Dhp
-