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Information on EC 3.2.1.22 - alpha-galactosidase
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3.2.1.22 alpha-galactosidaseIUBMB Comments
Also hydrolyses alpha-D-fucosides.
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Word Map
- 3.2.1.22
- fabry
- lysosomal
-
x-linked
- cardiac
- glycosphingolipids
- globotriaosylceramide
- pain
- galactose
- epitope
- endothelial
- oligosaccharide
- hypertrophy
- ventricular
- raffinose
- glycolipids
- proteinuria
- angiokeratomas
- melibiose
- glycosidases
- cerebrovascular
- alpha-glucosidase
-
hemizygotes
-
xenotransplantation
- stachyose
-
multisystemic
-
corporation
-
anti-gal
- galactomannans
- simplicifolia
-
cambridge
- alpha-l-fucosidase
-
hyperacute
- anaphylaxis
- coffee
- degradation
- arylamidase
- griffonia
- alpha-d-mannosidase
-
soymilk
- beta-d-galactosidase
- guar
-
sanofi
-
flatulence
- beta-d-glucosidase
- beta-mannosidase
- analysis
-
verticillata
- gaucher
- imiglucerase
- alpha-n-acetylgalactosaminidase
- biotechnology
- nutrition
- molecular biology
- medicine
-
bandeiraea
- beta-d-glucuronidase
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,6-alpha-D-galactoside galactohydrolase
Aga-F78
agaAJB13
AGal
Agalsidase alfa
AglC
alkaline alpha-galactosidase
alpha-D-galactopyranoside galactohydrolase
alpha-D-galactosidase
Alpha-D-galactoside galactohydrolase
alpha-Gal
alpha-Gal A
alpha-galactosidase
alpha-galactosidase A
alpha-galactosidase I
alpha-galactosidase II
alpha-galactoside galactohydrolase
alphaGal1
ceramidase, galactosylgalactosylglucosyl-
-
-
-
-
ceramide trihexosidase
-
-
-
-
ceramidetrihexosidase
-
-
-
-
ceramidetrihexoside-alpha-galactosidase
-
-
-
-
Gal36
GALA
GalS
GH36 alpha-galactosidase
GLA
MEL1
MelA
melibiase
TM1192
TmGalA
TnGalA
trihexosyl ceramide galactosidase
-
-
-
-
trihexosylceramide alpha-galactosidase
-
-
-
-
trihexosylceramidealpha-galactosidase
-
-
-
-
Agalsidase alfa
-
-
-
-
alpha-D-galactosidase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactoside galactohydrolase
-
-
-
-
melibiase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + H2O = D-galactose + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
-
-
-
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
also hydrolyses alpha-D-fucosides
-
-
-
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
the recombinant enzyme catalyzes the hydrolysis of phenyl R-galactosides via a redox elimination-addition mechanism involving oxidation of the hydroxyl group at C-3 and elimination of phenol across the C-1-C-2 bond to give an enzyme-bound glycal intermediate
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
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CAS REGISTRY NUMBER
COMMENTARY
9023-01-2
-
9025-35-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-naphthyl alpha-D-galactoside + H2O
1-naphthol + D-galactose
-
-
-
?
2,4,6-trinitrophenyl 2-deoxy-2,2-difluoro-alpha-D-galactopyranoside + H2O
2',4',6'-trinitrophenol + 2-deoxy-2,2-difluoro-alpha-D-galactopyranose
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactose
-
-
-
?
3-nitrophenyl alpha-D-galactoside + H2O
3-nitrophenol + alpha-D-galactose
O33835
-
-
-
?
4-bromophenyl alpha-D-galactoside + H2O
4-bromophenol + alpha-D-galactose
O33835
-
-
-
?
4-methoxyphenyl alpha-D-galactoside + H2O
4-methoxyphenol + alpha-D-galactose
O33835
-
-
-
?
4-methylumbelliferyl alpha-D-galactopyranoside + H2O
4-methylumbelliferone + alpha-D-galactopyranose
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
alpha-D-galactose + 4-methylumbelliferone
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
-
100% activity
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + alpha-D-xylopyranose
-
9.64% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
low activity
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
4-O-[4-O[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose
-
-
-
-
?
6-bromo-2-naphthyl alpha-D-galactoside + H2O
6-bromo-2-naphthol + D-galactose
-
-
-
-
?
6-bromo-2-naphthyl alpha-D-galactoside + H2O
6-bromo-2-naphthol + D-glucose
-
-
-
?
alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose
-
-
-
?
alpha-D-galactosyl fluoride + H2O
D-galactose + fluoride
-
-
-
?
beta-naphthyl alpha-D-galactoside + H2O
beta-naphthol + alpha-D-galactose
O33835
-
-
-
?
D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O
lactosyl-N-acylsphingosine + D-galactose
digalactosyldiacylglycerol + H2O
?
-
galactolipid substrate, located in chloroplast thylakoid membranes, converted by the purified recombinant cutinase (EC 3.1.1.74) expressed in tobacco chloroplasts and showing alpha galactosidase activity, EC 3.2.1.22
-
-
?
gentibiose-alternansucrase-derived oligosaccharide + ?
?
-
1.28fold increased activity compared to gentibiose
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
-
i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
-
-
?
maltitol-alternansucrase-derived oligosaccharide + ?
?
-
2.66fold increased activity compared to malitol
-
-
?
maltose-alternansucrase-derived oligosaccharide + ?
?
-
3.19fold increased activity compared to maltose
-
-
?
melibiose-alternansucrase-derived oligosaccharide + ?
?
-
1.45fold decreased activity compared to melibiose
-
-
?
N-acetyl-D-galactosamine + melibiose
6-O-alpha-D-galactosyl-N-acetyl-D-galactosamine + D-glucose
-
-
-
-
?
N-acetyl-D-glucosamine + melibiose
6-O-alpha-D-galactosyl-N-acetyl-D-glucosamine + H2O
-
-
-
-
?
O-beta-D-mannopyranosyl-(1-4)-O-[alpha-D-galactopyranosyl-(1-6)]-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
?
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + D-galactose
-
27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
o-nitrophenyl beta-D-galactopyranoside
o-nitrophenol + beta-D-galactopyranose
-
4.53% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose
?
-
transglycosylation
-
-
?
p-nitrophenyl alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
100% activity
-
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
enzyme P1: 5.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: no activity
-
-
?
p-nitrophenyl-beta-L-arabinopyranoside + H2O
p-nitrophenol + beta-L-arabinose
-
-
-
?
phenyl alpha-D-galactoside + H2O
phenol + alpha-D-galactose
O33835
-
-
-
?
raffinose-alternansucrase-derived oligosaccharide + ?
?
-
1.67fold increased activity compared to raffinose
-
-
?
2,4-dinitrophenyl alpha-D-galactoside + H2O
2,4-dinitrophenol + alpha-D-galactose
O33835
-
-
-
?
2,4-dinitrophenyl alpha-D-galactoside + H2O
2,4-dinitrophenol + alpha-D-galactose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
O33835
-
-
-
?
2,5-dinitrophenyl alpha-D-galactoside + H2O
2,5-dinitrophenol + alpha-D-galactose
O33835
-
-
-
?
2,5-dinitrophenyl alpha-D-galactoside + H2O
2,5-dinitrophenol + alpha-D-galactose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
O33835
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
-
16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
-
16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
-
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
-
-
-
-
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
O33835
-
-
-
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
O33835
-
-
-
?
3,4-dimethylphenyl alpha-D-galactoside + H2O
3,4-dimethylphenol + alpha-D-galactose
O33835
-
-
-
?
3,4-dimethylphenyl alpha-D-galactoside + H2O
3,4-dimethylphenol + alpha-D-galactose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
O33835
-
-
-
?
3-nitrophenyl alpha-D-galactopyranoside + H2O
3-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
3-nitrophenyl alpha-D-galactopyranoside + H2O
3-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
3-nitrophenyl alpha-D-galactopyranoside + H2O
3-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
3-nitrophenyl-alpha-D-galactopyranoside + H2O
3-nitrophenol + D-galactopyranose
-
5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
-
?
3-nitrophenyl-alpha-D-galactopyranoside + H2O
3-nitrophenol + D-galactopyranose
-
5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
-
-
-
-
?
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
-
-
-
-
?
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
Corynebacterium murisepticum
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
Penicillium chrysogenum sp. 23
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
highest activity
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
best substrate
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
best substrate
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
hydrolysis is accompanied by transglycosylation resulting in production of a mixture of (alpha1,2)-, (alpha1,3)-, and (alpha1,6)-4-nitrophenyl-digalactosides
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
hydrolysis is accompanied by transglycosylation resulting in production of a mixture of (alpha1,2)-, (alpha1,3)-, and (alpha1,6)-4-nitrophenyl-digalactosides
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
Tachigali multijuga
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
O33835
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
Q7WWP9
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
activity measured in total fly homogenates, substrate concentration of 60 mM in citrate buffer
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
extracellular enzyme
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
intracellular enzyme
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
extracellular enzyme
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
intracellular enzyme
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
-
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
-
-
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
-
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6-bromo-2-naphthyl-alpha-D-galactopyranoside + H2O
6-bromo-2-naphthol + D-galactose
-
-
-
?
6-bromo-2-naphthyl-alpha-D-galactopyranoside + H2O
6-bromo-2-naphthol + D-galactose
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
-
-
-
?
D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O
lactosyl-N-acylsphingosine + D-galactose
-
-
-
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?
D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O
lactosyl-N-acylsphingosine + D-galactose
-
-
-
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?
D-sucrose + H2O
D-glucose + D-fructose
-
35.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside
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-
?
D-sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
D-sucrose + H2O
D-glucose + D-fructose
-
16.30% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
galactomannan + H2O
?
-
with galactose/mannose ratios of 2.4 and of 1.72
-
-
?
guar gum + H2O
?
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
-
enzyme P1: 6.2% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
-
2.3% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
locust bean gum + H2O
?
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
m-chlorophenyl alpha-D-galactoside + H2O
m-chlorophenol + D-galactose
-
-
-
-
?
maltose + H2O
alpha-D-glucose
-
6.07% activity relative to p-nitrophenyl alpha-D-galactopyranoside
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-
?
maltose + H2O
alpha-D-glucose
-
enzyme P1: 4% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
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-
?
maltose + H2O
alpha-D-glucose
-
18.12% activity relative to p-nitrophenyl alpha-D-galactopyranoside
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-
?
maltose + H2O
alpha-D-glucose
-
10.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
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-
-
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?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
alpha-D-galactose + D-glucose
-
42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
alpha-D-galactose + D-glucose
-
enzyme P1: 6.9% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10% of the activity with p-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
alpha-D-galactose + D-glucose
-
35.5% of Vmax with p-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
D-galactose + D-glucose
-
15.36% activity relative to p-nitrophenyl alpha-D-galactopyranoside
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-
?
melibiose + H2O
D-galactose + D-glucose
-
intracellular enzyme
-
-
?
melibiose + H2O
D-galactose + D-glucose
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
-
7.68% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
-
16.15% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
-
negligible hydrolytic activity towards melibiose
-
-
?
melibiose + H2O
D-galactose + D-glucose
-
negligible hydrolytic activity towards melibiose
-
-
?
melibiose + H2O
D-galactose + D-glucose
-
7.3% of Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-glucose + D-galactose
-
15.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
-
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
-
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
-
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
Absidia sp. (in: Fungi) WL511
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-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
100% activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
100% activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
100% activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + D-galactose
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
alpha-galactosidase 1
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
alpha-galactosidase 2
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
alpha-galactosidase 3
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
-
?
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
-
-
-
-
?
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
Coffea sp.
-
-
-
-
?
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
-
-
-
-
?
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
-
-
-
-
?
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
-
-
-
-
?
raffinose + H2O
D-galactose + sucrose
-
assay at pH 7.5, 37°C, 15 min, reaction terminated by 2 min boiling
-
-
?
raffinose + H2O
D-galactose + sucrose
-
assay at pH 7.5, 37°C, 20 min, reaction terminated by keeping the reaction mixture in boiling water for 2 min
-
-
?
raffinose + H2O
D-galactose + sucrose
13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
D-galactose + sucrose
13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
-
52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
-
enzyme P1: 11.8% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 54.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
-
Vmax is 1.8fold higher than that with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
-
20% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
-
78.57% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
-
intracellular enzyme
-
-
?
raffinose + H2O
sucrose + D-galactose
-
49.95% activity compared to 4-nitrophenyl alpha-D-glucopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
-
30.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
-
60.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
high actvity with alpha-Gal I, low activity with alpha-Gal II
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
85.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
enzyme P1: 5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
11.74% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
78.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
Vmax is 2fold higher than that with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
17.8% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
20fold less active than with raffinose
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
-
20fold less active than with raffinose
-
-
?
stachyose + H2O
2 D-galactose + sucrose
-
6.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside
-
-
?
stachyose + H2O
raffinose + D-galactose
31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + H2O
raffinose + D-galactose
31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
-
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
20fold less active than with raffinose
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
-
-
-
?
additional information
?
-
-
pectin and polygalacturonic acid are not measurably degraded
-
-
?
additional information
?
-
no substrate: galactomannan
-
-
?
additional information
?
-
-
D-lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
?
additional information
?
-
-
activity is not enhanced on sucrose and beta-linked carbohydrates and fructooligosaccharides
-
-
?
additional information
?
-
under optimum pH conditions and high melibiose concentration of 40% w/v, the enzyme is able to form oligosaccharides with degree of polymerisation of 3 and more at higher concentration than with degree of polymerization 2, with a total yield of 20.5% w/w
-
-
?
additional information
?
-
-
under optimum pH conditions and high melibiose concentration of 40% w/v, the enzyme is able to form oligosaccharides with degree of polymerisation of 3 and more at higher concentration than with degree of polymerization 2, with a total yield of 20.5% w/w
-
-
?
additional information
?
-
-
raffinose induces about twice the activity than melibiose
-
-
?
additional information
?
-
enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors
-
-
?
additional information
?
-
no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside
-
-
?