Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
raffinose + H2O | Penicillium chrysogenum sp. 23 | - |
D-fructose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum sp. 23 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography and Sephadex G-100 gel filtration | Penicillium chrysogenum sp. 23 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.46 | - |
lyophilized extract, at pH 5.5 and 50°C | Penicillium chrysogenum sp. 23 |
30 | - |
after 65.2fold purification, at pH 5.5 and 50°C | Penicillium chrysogenum sp. 23 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Penicillium chrysogenum sp. 23 | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
raffinose + H2O | - |
Penicillium chrysogenum sp. 23 | D-fructose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-Gal | - |
Penicillium chrysogenum sp. 23 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium chrysogenum sp. 23 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | the enzyme is stable from 25 to 40°C for 24 h. At 45°C the activity falls to 80%, at 50°C to 72% and at 60°C the activity is completely lost | Penicillium chrysogenum sp. 23 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Penicillium chrysogenum sp. 23 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6 | the enzyme activity remains stable over a wide range of pH 4.5-6.0 for 24 h | Penicillium chrysogenum sp. 23 |