Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required for enzymatic activity | Citrobacter freundii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for enzymatic activity | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
raffinose + H2O = sucrose + alpha-D-galactopyranose | the recombinant enzyme catalyzes the hydrolysis of phenyl R-galactosides via a redox elimination-addition mechanism involving oxidation of the hydroxyl group at C-3 and elimination of phenol across the C-1-C-2 bond to give an enzyme-bound glycal intermediate | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Citrobacter freundii | 4-nitrophenol + alpha-D-galactopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-galactosidase | - |
Citrobacter freundii |
MelA | - |
Citrobacter freundii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Citrobacter freundii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 4.5, 37°C | Citrobacter freundii | |
0.23 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 9.0, 37°C | Citrobacter freundii | |
0.3 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 6.7, 37°C | Citrobacter freundii | |
0.62 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 8.7, 37°C | Citrobacter freundii | |
1.05 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 7.1, 37°C | Citrobacter freundii | |
1.37 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 8.4, 37°C | Citrobacter freundii | |
1.68 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 7.5, 37°C | Citrobacter freundii | |
1.75 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 8.0, 37°C | Citrobacter freundii | |
2.07 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 7.8, 37°C | Citrobacter freundii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required for enzymatic activity | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 4.5, 37°C | Citrobacter freundii | |
0.81 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 6.7, 37°C | Citrobacter freundii | |
0.96 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 9.0, 37°C | Citrobacter freundii | |
2.7 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 7.1, 37°C | Citrobacter freundii | |
5 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 8.7, 37°C | Citrobacter freundii | |
5.8 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 7.5, 37°C | Citrobacter freundii | |
8.9 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 8.0, 37°C | Citrobacter freundii | |
9.9 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 7.8, 37°C | Citrobacter freundii | |
15.6 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 8.4, 37°C | Citrobacter freundii |