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Literature summary for 3.2.1.22 extracted from
- Characterization of a GH36 alph-D-galactosidase associated with assimilation of gum arabic in Bifidobacterium longum subsp. longum JCM7052 (2021), J. Appl. Glycosci., 68, 47-52 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(lambdaDE3) cells | Bifidobacterium longum subsp. Longum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.774 | - |
alpha-D-Gal-(1->3)-L-Ara | at pH 6.0 and 40°C | Bifidobacterium longum subsp. Longum | |
| 29.5 | - |
raffinose | at pH 6.0 and 40°C | Bifidobacterium longum subsp. Longum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum subsp. Longum | - |
- |
- |
| Bifidobacterium longum subsp. Longum JCM7052 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal affinity resin column chromatography, and Superdex 200 gel filtration | Bifidobacterium longum subsp. Longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-galactoside | - |
Bifidobacterium longum subsp. Longum | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 4-nitrophenyl alpha-D-galactoside | - |
Bifidobacterium longum subsp. Longum JCM7052 | 4-nitrophenol + alpha-D-galactose | - |
? | |
| alpha-D-Gal-(1->3)-L-Ara + H2O | the enzyme preferentially cleaves alpha-D-Gal-(1->3)-L-Ara rather than beta-L-Arap-(1->3)-L-Ara in 3-O-alpha-D-galactosyl-alpha-L-arabinofuranosidase-releasing disaccharides | Bifidobacterium longum subsp. Longum | alpha-D-galactose + L-arabinose | - |
? | |
| alpha-D-Gal-(1->3)-L-Ara + H2O | the enzyme preferentially cleaves alpha-D-Gal-(1->3)-L-Ara rather than beta-L-Arap-(1->3)-L-Ara in 3-O-alpha-D-galactosyl-alpha-L-arabinofuranosidase-releasing disaccharides | Bifidobacterium longum subsp. Longum JCM7052 | alpha-D-galactose + L-arabinose | - |
? | |
| blood group B liner trisaccharide + H2O | - |
Bifidobacterium longum subsp. Longum | ? | - |
? | |
| blood group B liner trisaccharide + H2O | - |
Bifidobacterium longum subsp. Longum JCM7052 | ? | - |
? | |
| gum arabic arabinogalactan protein + H2O | - |
Bifidobacterium longum subsp. Longum | ? | - |
? | |
| melibiose + H2O | - |
Bifidobacterium longum subsp. Longum | alpha-D-galactose + D-glucose | - |
? | |
| melibiose + H2O | - |
Bifidobacterium longum subsp. Longum JCM7052 | alpha-D-galactose + D-glucose | - |
? | |
| additional information | the enzyme also performs transglycosylation reactions with 4-nitrophenyl alpha-D-galactoside and methanol, ethanol or 1-propanol as acceptor | Bifidobacterium longum subsp. Longum | ? | - |
- |
|
| additional information | the enzyme also performs transglycosylation reactions with 4-nitrophenyl alpha-D-galactoside and methanol, ethanol or 1-propanol as acceptor | Bifidobacterium longum subsp. Longum JCM7052 | ? | - |
- |
|
| raffinose + H2O | - |
Bifidobacterium longum subsp. Longum | alpha-D-galactose + sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, His-tagged enzyme, SDS-PAGE | Bifidobacterium longum subsp. Longum |
| ? | x * 79587, calculated from amino acid sequence | Bifidobacterium longum subsp. Longum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| blAga3 | - |
Bifidobacterium longum subsp. Longum |
| BLGA_00330 | - |
Bifidobacterium longum subsp. Longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bifidobacterium longum subsp. Longum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 107 | - |
alpha-D-Gal-(1->3)-L-Ara | at pH 6.0 and 40°C | Bifidobacterium longum subsp. Longum | |
| 270 | - |
raffinose | at pH 6.0 and 40°C | Bifidobacterium longum subsp. Longum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bifidobacterium longum subsp. Longum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.17 | - |
raffinose | at pH 6.0 and 40°C | Bifidobacterium longum subsp. Longum | |
| 138 | - |
alpha-D-Gal-(1->3)-L-Ara | at pH 6.0 and 40°C | Bifidobacterium longum subsp. Longum |
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