Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.22 extracted from

  • Yang, D.; Tian, G.; Du, F.; Zhao, Y.; Zhao, L.; Wang, H.; Ng, T.B.
    A fungal alpha-galactosidase from Pseudobalsamia microspora capable of degrading raffinose family oligosaccharides (2015), Appl. Biochem. Biotechnol., 176, 2157-2169 .
    View publication on PubMed
Search for more literature for 3.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
carbodiimide 165% activity at 0.4 mM Diehliomyces microsporus
diacetyl 140% activity at 0.2 mM Diehliomyces microsporus
diethyl dicarbonate 170% activity at 0.4 mM Diehliomyces microsporus

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 46% residual activity at 2.5 mM Diehliomyces microsporus
Ca2+ 47% residual activity at 2.5 mM Diehliomyces microsporus
Cd2+ complete inhibition at 1.25 mM Diehliomyces microsporus
Cu2+ complete inhibition at 1.25 mM Diehliomyces microsporus
dithiothreitol 54% residual activity at 0.4 mM Diehliomyces microsporus
Fe2+ 44% residual activity at 1.25 mM Diehliomyces microsporus
Fe3+ 6% residual activity at 10 mM Diehliomyces microsporus
Hg2+ complete inhibition at 1.25 mM Diehliomyces microsporus
K+ 32% residual activity at 2.5 mM Diehliomyces microsporus
Mg2+ 63% residual activity at 1.25 mM Diehliomyces microsporus
Mn2+ 33% residual activity at 5 mM Diehliomyces microsporus
N-bromosuccinimide complete inhibition at 0.2 mM Diehliomyces microsporus
Pb2+ 29% residual activity at 5 mM Diehliomyces microsporus
Zn2+ 24% residual activity at 5 mM Diehliomyces microsporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 4-nitrophenyl alpha-D-glucopyranoside at pH 4.6 and 50°C Diehliomyces microsporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Diehliomyces microsporus

Organism

Organism UniProt Comment Textmining
Diehliomyces microsporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, Q Sepharose column chromatography, Mono Q column chromatography, and Superdex G-75 gel filtration Diehliomyces microsporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.21
-
 crude extract, at pH 4.6 and 50°C Diehliomyces microsporus
11275
-
 after 1224.1fold purification, at pH 4.6 and 50°C Diehliomyces microsporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O 100% activity Diehliomyces microsporus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
melibiose + H2O 15.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside Diehliomyces microsporus D-glucose + D-galactose
-
 ?
additional information no activity with 2-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucuronide, guar gum, and locust bean gum Diehliomyces microsporus ?
-
 ?
raffinose + H2O 49.95% activity compared to 4-nitrophenyl alpha-D-glucopyranoside Diehliomyces microsporus sucrose + D-galactose
-
 ?
stachyose + H2O 6.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside Diehliomyces microsporus 2 D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE Diehliomyces microsporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Diehliomyces microsporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 about 40% activity at 30°C, about 55% activity at 40°C, about 95% activity at 50°C, 100% activity at 55°C, about 80% activity at 60°C, almost no activity at 70°C Diehliomyces microsporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Diehliomyces microsporus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 the enzyme exhibits 60% and 73% of the maximum activity at pH 4.0 and 6.0, respectively. At pH 3.0 and 7.0, there is hardly any alpha-galactosidase activity remaining Diehliomyces microsporus