Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5, 37°C | Aspergillus nidulans | |
12 | - |
melibiose | pH 5, 37°C | Aspergillus nidulans | |
15 | - |
raffinose | pH 5, 37°C | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
SDS-PAGE | Aspergillus nidulans |
335000 | - |
gel filtration | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Aspergillus nidulans | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
melibiose + H2O | - |
Aspergillus nidulans | D-galactose + D-glucose | - |
? | |
raffinose + H2O | - |
Aspergillus nidulans | sucrose + alpha-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 83000, SDS-PAGE | Aspergillus nidulans |
Synonyms | Comment | Organism |
---|---|---|
AglC | - |
Aspergillus nidulans |
alpha-galactosidase | - |
Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus nidulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
retains more than 95% activity after 15 min incubation up to 45°C at pH 5.0 | Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
586 | - |
raffinose | pH 5, 37°C | Aspergillus nidulans | |
1067 | - |
melibiose | pH 5, 37°C | Aspergillus nidulans | |
1278 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5, 37°C | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus nidulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.6 | 9.9 | - |
Aspergillus nidulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
39.1 | - |
raffinose | pH 5, 37°C | Aspergillus nidulans | |
88.9 | - |
melibiose | pH 5, 37°C | Aspergillus nidulans | |
4730 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5, 37°C | Aspergillus nidulans |