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Literature summary for 3.2.1.22 extracted from

  • Peters, S.; Egert, A.; Stieger, B.; Keller, F.
    Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline alpha-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern (2010), Plant Cell Physiol., 51, 1815-1819.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
1-deoxygalactonojirimycin
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
105
-
raffinose pH 7.5, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information a beta-glucuronidase reporter construct using the ATSIP2 promoter shows that ATSIP2 is strongly expressed in sink tissues of Arabidopsis, i.e. sink leaves and non-xylem parts of the root stele Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Arabidopsis thaliana 4-nitrophenol + alpha-D-galactopyranose
-
?
raffinose + H2O
-
Arabidopsis thaliana D-galactose + sucrose
-
?

Synonyms

Synonyms Comment Organism
alpha-galactosidase
-
Arabidopsis thaliana
ATSIP2
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8
-
Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function raffinose phloem unloading Arabidopsis thaliana