Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Limosilactobacillus fermentum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 90.4% residual activity at 1 mM | Limosilactobacillus fermentum | |
Cd2+ | 86.0% residual activity at 1 mM | Limosilactobacillus fermentum | |
Cu2+ | 76.8% residual activity at 1 mM | Limosilactobacillus fermentum | |
Hg+ | 20.9% residual activity at 1 mM | Limosilactobacillus fermentum | |
Li+ | 93.3% residual activity at 1 mM | Limosilactobacillus fermentum | |
Mg2+ | 92.6% residual activity at 1 mM | Limosilactobacillus fermentum | |
Mn2+ | 91.5% residual activity at 1 mM | Limosilactobacillus fermentum | |
Zn2+ | 78.3% residual activity at 1 mM | Limosilactobacillus fermentum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
4 * 84000, ultrafiltration | Limosilactobacillus fermentum |
336000 | - |
native enzyme, ultrafiltration | Limosilactobacillus fermentum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus fermentum | Q6IYF5 | - |
- |
Limosilactobacillus fermentum CRL722 | Q6IYF5 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose Fast Flow column chromatography and ammonium sulfate precipitation | Limosilactobacillus fermentum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
cell-free extract | Limosilactobacillus fermentum |
4.37 | - |
after 20.8fold purification | Limosilactobacillus fermentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + phenyl alpha-D-galactoside | - |
Limosilactobacillus fermentum | ? | - |
? | |
D-galactose + phenyl alpha-D-galactoside | - |
Limosilactobacillus fermentum CRL722 | ? | - |
? | |
melibiose + H2O | - |
Limosilactobacillus fermentum | D-galactose + D-glucose | - |
? | |
melibiose + H2O | - |
Limosilactobacillus fermentum CRL722 | D-galactose + D-glucose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Limosilactobacillus fermentum | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Limosilactobacillus fermentum CRL722 | p-nitrophenol + alpha-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 84000, ultrafiltration | Limosilactobacillus fermentum |
Synonyms | Comment | Organism |
---|---|---|
alpha-Gal | - |
Limosilactobacillus fermentum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Limosilactobacillus fermentum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 52 | 50% activity is observed between 35°C and 50°C | Limosilactobacillus fermentum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | the enzyme is active at moderately high temperatures (55°C), no activity loss is observed after 30 min of incubation at 50°C, at 25°C the enzyme still keeps 20% activity, whereas at temperatures above 55°C it drastically decreases | Limosilactobacillus fermentum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Limosilactobacillus fermentum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 6.5 | more than 50% activity between pH 4.3 and 6.5 | Limosilactobacillus fermentum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
20% activity at pH 8.0 | Limosilactobacillus fermentum |