Literature summary for 3.2.1.22 extracted from

  • Dey, P.M.; Pridham, J.B.
    Biochemistry of alpha-galactosidases (1972), Adv. Enzymol. Relat. Areas Mol. Biol., 36, 91-130.
    View publication on PubMed

General Stability

General Stability Organism
Mn2+ and reducing agents stabilizes activity in cell free extract Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Medicago sativa
Ag+
-
Prunus dulcis
Ag+
-
Vicia faba
Cu2+
-
Prunus dulcis
Hg2+
-
Medicago sativa
Hg2+
-
Prunus dulcis
Hg2+
-
Umbelopsis vinacea
Hg2+
-
Vicia faba
iodacetamide
-
Klebsiella aerogenes
iodacetamide
-
Streptococcus pneumoniae
iodacetamide
-
Streptomyces olivaceus
N-ethyl maleimide
-
Klebsiella aerogenes
N-ethyl maleimide
-
Streptococcus pneumoniae
N-ethyl maleimide
-
Streptomyces olivaceus
p-chloromercuribenzoate
-
Klebsiella aerogenes
p-chloromercuribenzoate
-
Streptococcus pneumoniae
p-chloromercuribenzoate
-
Streptomyces olivaceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.87
-
p-aminophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
0.45
-
p-nitrophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
1
-
p-cresyl alpha-D-galactoside alpha-galactosidase II Vicia faba
2
-
m-cresyl alpha-D-galactoside alpha-galactosidase II Vicia faba
8
-
ethyl alpha-D-galactoside alpha-galactosidase II Vicia faba
0.78
-
o-cresyl alpha-D-galactoside alpha-galactosidase II Vicia faba
6.25
-
ethyl alpha-D-galactoside
-
Prunus dulcis
6.25
-
n-propyl alpha-D-galactoside
-
Prunus dulcis
12.5
-
raffinose
-
Prunus dulcis
12.5
-
p-nitrophenyl beta-L-arabinoside alpha-galactosidase II Vicia faba
1.33
-
o-cresyl alpha-D-galactoside alpha-galactosidase I Vicia faba
1.8
-
6-bromo-2-naphthyl alpha-D-galactoside alpha-galactosidase I Vicia faba
10
-
m-nitrophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
2.5
-
m-nitrophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
0.62
-
6-bromo-2-naphthyl alpha-D-galactoside alpha-galactosidase II Vicia faba
1.54
-
p-cresyl alpha-D-galactoside alpha-galactosidase I Vicia faba
0.33
-
o-nitrophenyl alpha-D-galactoside
-
Prunus dulcis
0.77
-
melibiose alpha-galactosidase II Vicia faba
4
-
raffinose alpha-galactosidase I Vicia faba
0.13
-
galactinol alpha-galactosidase I Vicia faba
5.26
-
stachyose alpha-galactosidase II Vicia faba
0.38
-
p-nitrophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
0.69
-
galactinol alpha-galactosidase II Vicia faba
0.69
-
o-nitrophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
5
-
phenyl alpha-D-galactoside
-
Prunus dulcis
5
-
raffinose alpha-galactosidase II Vicia faba
0.83
-
m-chlorophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
0.53
-
p-nitrophenyl alpha-D-galactoside
-
Prunus dulcis
1.38
-
m-cresyl alpha-D-galactoside alpha-galactosidase I Vicia faba
0.95
-
p-aminophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
7.5
-
stachyose alpha-galactosidase I Vicia faba
0.96
-
melibiose alpha-galactosidase I Vicia faba
33.3
-
p-nitrophenyl beta-L-arabinoside
-
Prunus dulcis
1.25
-
phenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
14.3
-
p-nitrophenyl beta-L-arabinoside alpha-galactosidase I Vicia faba
14.3
-
methyl alpha-D-galactoside alpha-galactosidase II Vicia faba
10.9
-
methyl alpha-D-galactoside
-
Prunus dulcis
1.11
-
phenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
1.14
-
o-nitrophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
8.33
-
m-chlorophenyl alpha-D-galactoside
-
Prunus dulcis
8.33
-
m-cresyl alpha-D-galactoside
-
Prunus dulcis
1.17
-
m-chlorophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
1.57
-
m-nitrophenyl alpha-D-galactoside
-
Prunus dulcis
4.76
-
p-cresyl alpha-D-galactoside
-
Prunus dulcis
4.76
-
p-nitrophenyl alpha-D-fucoside alpha-galactosidase I Vicia faba
2.24
-
melibiose
-
Prunus dulcis
8.93
-
ethyl alpha-D-galactoside alpha-galactosidase I Vicia faba
7.13
-
methyl alpha-D-galactoside alpha-galactosidase I Vicia faba
6.13
-
n-propyl alpha-D-galactoside alpha-galactosidase I Vicia faba
5.88
-
n-propyl alpha-D-galactoside alpha-galactosidase II Vicia faba
5.88
-
p-nitrophenyl alpha-D-fucoside alpha-galactosidase II Vicia faba
4.54
-
o-cresyl alpha-D-galactoside
-
Prunus dulcis

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound
-
Sus scrofa
-
-
mitochondrion
-
Rattus norvegicus
-
-
mitochondrion
-
Spinacia oleracea
-
-
cytoplasm
-
Rattus norvegicus
-
-
chloroplast
-
Spinacia oleracea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates the enzyme Vicia faba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Medicago sativa
38000
-
alpha-galactosidase II, gel filtration Vigna radiata var. radiata
38000
-
alpha-galactosidase II, gel filtration Vicia faba
50000
-
alpha-galactosidase II, gel filtration Acer pseudoplatanus
195000
-
alpha-galactosidase I, gel filtration Vicia dumetorum
57000
-
alpha-galactosidase II, gel filtration Vicia dumetorum
77000
-
dormant seeds, alpha-galactosidase II, gel filtration Vicia sativa
23000
-
gel filtration Helianthus annuus
25000
-
gel filtration Cyamopsis tetragonoloba
30000
-
germinated seeds, gel filtration Vicia sativa
167000
-
alpha-galactosidase I, gel filtration Acer pseudoplatanus
26000
-
gel filtration Coffea sp.
87000
-
gel filtration Cajanus indicus
159000
-
alpha-galactosidase I, gel filtration Helianthus annuus
209000
-
alpha-galactosidase I, gel filtration Vigna radiata var. radiata
209000
-
alpha-galactosidase I, gel filtration Vicia faba
166000
-
dormant seeds, alpha-galactosidase I, gel filtration Vicia sativa
125900
-
alpha-galactosidase I, gel filtration Phaseolus vulgaris
121600
-
alpha-galactosidase I, gel filtration Pisum sativum
39800
-
alpha-galactosidase II, gel filtration Phaseolus vulgaris
32300
-
alpha-galactosidase II, gel filtration Pisum sativum
30000 32400 gel filtration Prunus dulcis

Organism

Organism UniProt Comment Textmining
Acer pseudoplatanus
-
-
-
Klebsiella aerogenes
-
-
-
Aspergillus niger
-
-
-
Aspergillus paxillus
-
-
-
Bos taurus
-
-
-
Brassica oleracea
-
-
-
Vicia faba
-
-
-
Cajanus indicus
-
-
-
Calvatia cyanthiformis
-
-
-
Canavalia ensiformis
-
-
-
Ricinus communis
-
-
-
Citrullus lanatus subsp. vulgaris
-
-
-
Coffea sp.
-
green coffee beans
-
Cyamopsis tetragonoloba
-
-
-
Streptococcus pneumoniae
-
-
-
Escherichia coli
-
-
-
Epidinium ecaudatum
-
-
-
Eudiplodinium maggii
-
-
-
Helianthus annuus
-
-
-
Helix pomatia
-
-
-
Hordeum sp.
-
-
-
Medicago sativa
-
-
-
Melolontha melolontha
-
-
-
Umbelopsis vinacea
-
-
-
Ophryoscolex caudatus
-
-
-
Vigna radiata var. radiata
-
-
-
Phaseolus vulgaris
-
-
-
Pisum sativum
-
-
-
Plantago ovata
-
-
-
Polyplastron multivesiculatum
-
-
-
Prunus dulcis
-
-
-
Raphanus sativus
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces sp.
-
yeast
-
Spinacia oleracea
-
-
-
Streptococcus equinus
-
-
-
Streptomyces olivaceus
-
-
-
Sus scrofa
-
-
-
Tritrichomonas suis
-
-
-
Trigonella foenum-graecum
-
-
-
Triticum sp.
-
-
-
Vicia dumetorum
-
-
-
Vicia sativa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus niger
glycoprotein 2.7% D-glucosamine and 10.8% hexoses Umbelopsis vinacea
glycoprotein alpha-galactosidase I Vicia faba

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-
liver
-
Bos taurus
-
thyroid gland
-
Rattus norvegicus
-
uterus
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
parathyroid gland
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
stachyose + H2O
-
Vicia faba ?
-
?
stachyose + H2O
-
Escherichia coli ?
-
?
stachyose + H2O
-
Coffea sp. ?
-
?
stachyose + H2O
-
Umbelopsis vinacea ?
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
-
Vicia faba p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
-
Aspergillus niger p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
-
Medicago sativa p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
-
Prunus dulcis p-nitrophenol + alpha-D-galactose
-
?
melibiose + H2O
-
Vicia faba D-galactose + D-glucose
-
?
melibiose + H2O
-
Vicia sativa D-galactose + D-glucose
-
?
melibiose + H2O
-
Prunus dulcis D-galactose + D-glucose
-
?
melibiose + H2O
-
Coffea sp. D-galactose + D-glucose
-
?
melibiose + H2O
-
Saccharomyces sp. D-galactose + D-glucose
-
?
melibiose + H2O
-
Umbelopsis vinacea D-galactose + D-glucose
-
?
melibiose + H2O
-
Vicia dumetorum D-galactose + D-glucose
-
?
galactinol + H2O
-
Vicia faba alpha-D-galactopyranose + myoinositol
-
?
raffinose + H2O
-
Vicia faba sucrose + D-galactose
-
?
raffinose + H2O
-
Vicia sativa sucrose + D-galactose
-
?
raffinose + H2O
-
Escherichia coli sucrose + D-galactose
-
?
raffinose + H2O
-
Aspergillus niger sucrose + D-galactose
-
?
raffinose + H2O
-
Medicago sativa sucrose + D-galactose
-
?
raffinose + H2O
-
Prunus dulcis sucrose + D-galactose
-
?
raffinose + H2O
-
Coffea sp. sucrose + D-galactose
-
?
raffinose + H2O
-
Umbelopsis vinacea sucrose + D-galactose
-
?
raffinose + H2O
-
Vicia dumetorum sucrose + D-galactose
-
?
p-nitrophenyl beta-L-arabinoside + H2O
-
Vicia faba p-nitrophenol + L-arabinose
-
?
p-nitrophenyl beta-L-arabinoside + H2O
-
Prunus dulcis p-nitrophenol + L-arabinose
-
?
methyl alpha-D-galactoside + H2O
-
Vicia faba methanol + D-galactose
-
?
methyl alpha-D-galactoside + H2O
-
Prunus dulcis methanol + D-galactose
-
?
m-nitrophenyl alpha-D-galactoside + H2O
-
Vicia faba m-nitrophenol + D-galactose
-
?
m-nitrophenyl alpha-D-galactoside + H2O
-
Prunus dulcis m-nitrophenol + D-galactose
-
?
ethyl alpha-D-galactoside + H2O
-
Vicia faba ethanol + D-galactose
-
?
ethyl alpha-D-galactoside + H2O
-
Prunus dulcis ethanol + D-galactose
-
?
m-cresyl alpha-D-galactoside + H2O
-
Vicia faba m-cresol + D-galactose
-
?
m-cresyl alpha-D-galactoside + H2O
-
Prunus dulcis m-cresol + D-galactose
-
?
m-chlorophenyl alpha-D-galactoside + H2O
-
Vicia faba m-chlorophenol + D-galactose
-
?
m-chlorophenyl alpha-D-galactoside + H2O
-
Prunus dulcis m-chlorophenol + D-galactose
-
?
n-propyl alpha-D-galactoside + H2O
-
Vicia faba n-propanol + D-galactose
-
?
n-propyl alpha-D-galactoside + H2O
-
Prunus dulcis n-propanol + D-galactose
-
?
phenyl alpha-D-galactoside + H2O
-
Vicia faba phenol + D-galactose
-
?
phenyl alpha-D-galactoside + H2O
-
Prunus dulcis phenol + D-galactose
-
?
p-cresyl alpha-D-galactoside + H2O
-
Vicia faba p-cresol + D-galactose
-
?
p-cresyl alpha-D-galactoside + H2O
-
Prunus dulcis p-cresol + D-galactose
-
?
o-cresyl alpha-D-galactoside + H2O
-
Vicia faba o-cresol + D-galactose
-
?
o-cresyl alpha-D-galactoside + H2O
-
Prunus dulcis o-cresol + D-galactose
-
?
o-nitrophenyl alpha-D-galactoside + H2O
-
Vicia faba o-nitrophenol + D-galactose
-
?
o-nitrophenyl alpha-D-galactoside + H2O
-
Prunus dulcis o-nitrophenol + D-galactose
-
?
6-bromo-2-naphthyl alpha-D-galactoside + H2O
-
Vicia faba 6-bromo-2-naphthol + D-galactose
-
?
p-aminophenyl alpha-D-galactoside + H2O
-
Vicia faba p-aminophenol + D-galactose
-
?
p-nitrophenyl alpha-D-fucoside + H2O
-
Vicia faba p-nitrophenol + D-fucose
-
?
phenyl alpha-D-galactoside + methanol
-
Trigonella foenum-graecum methyl alpha-D-galactoside + phenol
-
?
phenyl alpha-D-galactoside + methanol
-
Brassica oleracea methyl alpha-D-galactoside + phenol
-
?
phenyl alpha-D-galactoside + methanol
-
Helianthus annuus methyl alpha-D-galactoside + phenol
-
?
phenyl alpha-D-galactoside + methanol
-
Coffea sp. methyl alpha-D-galactoside + phenol
-
?
phenyl alpha-D-galactoside + methanol
-
Hordeum sp. methyl alpha-D-galactoside + phenol
-
?
melibiose + methanol
-
Coffea sp. methyl alpha-D-galactoside + D-glucose
-
?
raffinose + methanol
-
Coffea sp. methyl alpha-D-galactoside + verbascose + ajugose
-
?
stachyose + methanol
-
Coffea sp. methyl alpha-D-galactoside + verbascose + ajugose
-
?
D-glucose + phenyl alpha-D-galactoside
-
Vicia sativa ?
-
?
D-glucose + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
D-glucose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
D-glucose + phenyl alpha-D-galactoside
-
Plantago ovata ?
-
?
D-galactose + phenyl alpha-D-galactoside
-
Phaseolus vulgaris ?
-
?
D-galactose + phenyl alpha-D-galactoside
-
Vicia sativa ?
-
?
D-galactose + phenyl alpha-D-galactoside
-
Ricinus communis ?
-
?
D-galactose + phenyl alpha-D-galactoside
-
Raphanus sativus ?
-
?
D-galactose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
D-galactose + phenyl alpha-D-galactoside
-
Plantago ovata ?
-
?
gentianose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
gentiobiose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
lactose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
maltose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
D-mannose + phenyl alpha-D-galactoside
-
Vicia sativa epimelibiose + H2O
-
?
D-mannose + phenyl alpha-D-galactoside
-
Coffea sp. epimelibiose + H2O
-
?
D-mannose + phenyl alpha-D-galactoside
-
Plantago ovata epimelibiose + H2O
-
?
cellobiose + phenyl alpha-D-galactoside
-
Coffea sp. ?
-
?
glycerol + melibiose
-
Coffea sp. floridoside + H2O
-
?
sucrose + melibiose
-
Coffea sp. raffinose + stachyose + planteose
-
?
sucrose + phenyl alpha-D-galactoside
-
Coffea sp. raffinose + stachyose + planteose
-
?
ethanol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
n-propanol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
n-propanol + phenyl alpha-D-galactoside
-
Brassica oleracea ?
-
?
n-propanol + phenyl alpha-D-galactoside
-
Helianthus annuus ?
-
?
n-propanol + phenyl alpha-D-galactoside
-
Hordeum sp. ?
-
?
n-butanol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
isopropanol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
isopentanol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
glycerol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
glycerol + phenyl alpha-D-galactoside
-
Brassica oleracea ?
-
?
glycerol + phenyl alpha-D-galactoside
-
Helianthus annuus ?
-
?
glycerol + phenyl alpha-D-galactoside
-
Hordeum sp. ?
-
?
mannitol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
sorbitol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
meso-inositol + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
sucrose + phenyl alpha-D-galactoside
-
Vicia sativa ?
-
?
sucrose + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
sucrose + phenyl alpha-D-galactoside
-
Plantago ovata ?
-
?
trehalose + phenyl alpha-D-galactoside
-
Trigonella foenum-graecum ?
-
?
sucrose + galactinol
-
Phaseolus vulgaris raffinose + H2O
-
?
raffinose + galactinol
-
Phaseolus vulgaris stachyose + H2O
-
?
melibiose + melibiose
-
Streptococcus pneumoniae ?
-
?
raffinose + raffinose
-
Streptococcus pneumoniae ?
-
?
D-glucose + melibiose
-
Streptococcus pneumoniae melibiose + D-glucose
-
?
D-glucose + raffinose
-
Streptococcus pneumoniae ?
-
?
stachyose + stachyose
-
Streptococcus pneumoniae ?
-
?
melibiose + melibiose
-
Streptococcus equinus mannotriose + D-glucose
-
?
sucrose + melibiose
-
Streptococcus equinus raffinose + D-glucose
-
?
raffinose + melibiose
-
Streptococcus equinus stachyose + H2O
-
?
1-naphthyl alpha-D-galactoside + H2O
-
Saccharomyces sp. 1-naphthol + D-galactose
-
?
2-naphthyl alpha-D-galactoside + H2O
-
Saccharomyces sp. 2-naphthol + D-glucose
-
?
sucrose + raffinose
-
Triticum sp. planteose + D-glucose
-
?
N-acetyl-D-glucosamine + melibiose
-
Tritrichomonas suis 6-O-alpha-D-galactosyl-N-acetyl-D-glucosamine + H2O
-
?
N-acetyl-D-galactosamine + melibiose
-
Tritrichomonas suis 6-O-alpha-D-galactosyl-N-acetyl-D-galactosamine + D-glucose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
60 min, 30% loss in activity Streptococcus pneumoniae
55
-
15 min, 90% loss of activity Streptomyces olivaceus
55
-
60 min, 35% loss in activity Aspergillus niger
55
-
5 min, 20% loss in activity Bos taurus
70
-
30 min, complete loss in activity Helix pomatia
62
-
5 min, 20% loss in activity Canavalia ensiformis
60
-
5 min, complete loss in activity Bos taurus
60
-
20 min, 15% loss of activity Prunus dulcis
60
-
alpha-galactosidase I, 30 min, 42% loss in activity, alpha-galactosidase II, 30 min, 81% loss of activity Vicia faba
75
-
40 min, 16% loss in activity Vicia sativa
43
-
10 min, 50% loss in activity Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Escherichia coli
6.3
-
-
Vicia sativa
6.3
-
-
Coffea sp.
6 6.5 alpha-galactosidase I, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
6
-
-
Melolontha melolontha
6
-
alpha-galactosidase II, phenyl alpha-D-galactoside as substrate Coffea sp.
4.2
-
-
Citrullus lanatus subsp. vulgaris
5.6
-
-
Ophryoscolex caudatus
5.9
-
alpha-galactosidase I Plantago ovata
5 5.5
-
Epidinium ecaudatum
5 5.5 alpha-galactosidase II, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
5 5.5 alpha-galactosidase II Pisum sativum
5.3
-
-
Spinacia oleracea
5.3
-
-
Polyplastron multivesiculatum
5.3
-
alpha-galactosidase II Plantago ovata
5.3
-
alpha-galactosidase I, phenyl alpha-D-galactoside as substrate Coffea sp.
4 5 p-nitrophenyl alpha-D-galactoside as substrate Canavalia ensiformis
4 6
-
Streptomyces olivaceus
4 6 stachyose or o-nitrophenyl alpha-D-galactoside as substrate Umbelopsis vinacea
4 4.5 kidney Rattus norvegicus
5.2
-
-
Sus scrofa
5.2
-
uterus Rattus norvegicus
5.4
-
-
Streptomyces olivaceus
5.6 6
-
Streptococcus pneumoniae
3.2
-
-
Trigonella foenum-graecum
4.6
-
-
Trigonella foenum-graecum
4.6
-
phenyl alpha-D-galactoside as substrate Aspergillus paxillus
5.1
-
-
Eudiplodinium maggii
3 3.5 alpha-galactosidase II Pisum sativum
3 3.5 alpha-galactosidase I, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
2.5 3.5 alpha-galactosidase II, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
6.5 6.7
-
Phaseolus vulgaris
3.5 4.5 1-naphthyl alpha-D-galactoside, 2-naphthyl alpha-D-galactoside or 6-bromo-2-naphthyl alpha-D-galactoside as substrate Saccharomyces sp.
3.5 4.5 alpha-galactosidase II, raffinose as substrate Vicia faba
5.5 5.7
-
Prunus dulcis
4.9
-
brain Rattus norvegicus
3.5 5.5 alpha-galactosidase I, raffinose as substrate Vicia faba
3 5 o-nitrophenyl alpha-D-galactoside as substrate Calvatia cyanthiformis
3 5 raffinose as substrate Umbelopsis vinacea
3 6.5 alpha-galactosidase I Pisum sativum
3.2 3.8
-
Helix pomatia
3.5 5.3 melibiose as substrate Saccharomyces sp.
3.6 4.2
-
Coffea sp.
3.8 4.5 p-nitrophenyl alpha-D-galactoside as substrate Aspergillus niger
4.2 4.8 melibiitol as substrate Aspergillus niger
5.6 6.3
-
Streptococcus equinus