Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 114.7% activity at 10 mM | Sphingomonas sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sphingomonas sp. |
General Stability | Organism |
---|---|
the enzyme shows strong resistance to trypsin and proteinase K digestion. After treatment at 37°C for 1 h with trypsin and proteinase K, the enzyme retained 102.1% and 114% of its initial activity, respectively | Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 10 mM | Sphingomonas sp. | |
FeSO4 | 69.5% residual acitivty at 10 mM | Sphingomonas sp. | |
Hg2+ | complete inhibition at 10 mM | Sphingomonas sp. | |
Mg2+ | 93.4% residual activity at 10 mM | Sphingomonas sp. | |
additional information | not inhibited by EDTA, 2-mercaptoethanol | Sphingomonas sp. | |
SDS | 7.6% residual acitivty at 10 mM | Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
4-nitrophenyl alpha-D-galactopyranoside | at pH 5.0 and 60°C | Sphingomonas sp. | |
29.4 | - |
melibiose | at pH 5.0 and 60°C | Sphingomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 119.7% activity at 10 mM | Sphingomonas sp. | |
Mn2+ | 110.8% activity at 10 mM | Sphingomonas sp. | |
additional information | not influenced by Zn2+, Fe3+, K+, and Cu2+ | Sphingomonas sp. | |
Na+ | 104.9% activity at 10 mM | Sphingomonas sp. | |
Ni2+ | 113.7% activity at 10 mM | Sphingomonas sp. | |
Pb2+ | 113.5% activity at 10 mM | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82300 | - |
x * 82300, calculated from amino acid sequence | Sphingomonas sp. |
85500 | - |
x * 85500, SDS-PAGE | Sphingomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | Sphingomonas sp. | - |
D-galactose + D-glucose | - |
? | |
melibiose + H2O | Sphingomonas sp. JB13 | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | M4GRS1 | - |
- |
Sphingomonas sp. JB13 | M4GRS1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Sphingomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
with raffinose as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
2.8 | - |
with cottonseed meal as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
6.9 | - |
with soybean meal as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
221 | - |
with melibiose as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
225 | - |
with 4-nitrophenyl alpha-D-galactopyranoside as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | best substrate | Sphingomonas sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
4-nitrophenyl alpha-D-galactopyranoside + H2O | best substrate | Sphingomonas sp. JB13 | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
cottonseed meal + H2O | - |
Sphingomonas sp. | ? | - |
? | |
cottonseed meal + H2O | - |
Sphingomonas sp. JB13 | ? | - |
? | |
melibiose + H2O | - |
Sphingomonas sp. | D-galactose + D-glucose | - |
? | |
melibiose + H2O | - |
Sphingomonas sp. JB13 | D-galactose + D-glucose | - |
? | |
raffinose + H2O | worst substrate | Sphingomonas sp. | sucrose + D-galactose | - |
? | |
raffinose + H2O | worst substrate | Sphingomonas sp. JB13 | sucrose + D-galactose | - |
? | |
soybean meal + H2O | - |
Sphingomonas sp. | ? | - |
? | |
soybean meal + H2O | - |
Sphingomonas sp. JB13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82300, calculated from amino acid sequence | Sphingomonas sp. |
? | x * 85500, SDS-PAGE | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
1,6-alpha-D-galactoside galactohydrolase | - |
Sphingomonas sp. |
agaAJB13 | - |
Sphingomonas sp. |
melibiase | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Sphingomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | more than 50% activity between 30 and 65°C | Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme has a half-life of more than 60 min at 60°C and retains about 25% activity at 20°C, and about 10% at 10°C. At 65°C and 70°C, the enzyme activity decreases rapidly | Sphingomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
448 | - |
melibiose | at pH 5.0 and 60°C | Sphingomonas sp. | |
512 | - |
4-nitrophenyl alpha-D-galactopyranoside | at pH 5.0 and 60°C | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Sphingomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | more than 50% activity between pH 4.5 and 6.5 | Sphingomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | the enzyme exhibits more than 40% of its initial activity after incubation in buffers ranging from pH 4.0 to 11.0 at 37°C for 1 h | Sphingomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sphingomonas sp. | calculated from amino acid sequence | - |
5.2 |