Literature summary for 3.2.1.22 extracted from

Guce, A.I.; Clark, N.E.; Salgado, E.N.; Ivanen, D.R.; Kulminskaya, A.A.; Brumer, H.; Garman, S.C.
Catalytic mechanism of human alpha-galactosidase (2010), J. Biol. Chem., 285, 3625-3632.

Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Trichoplusia ni insect cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 25% (w/v) PEG 4000, 200 mM (NH4)2SO4, and 100 mM NaCH3COO, pH 4.6, at 20°C	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D170A	the mutant protein lacks the active site nucleophile	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
melibiose + H2O	Homo sapiens	-	D-galactose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P06280	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-Sepharose column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-D-galactoside + H2O	-	Homo sapiens	4-nitrophenol + alpha-D-galactose	-	?
melibiose + H2O	-	Homo sapiens	D-galactose + D-glucose	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Homo sapiens

Synonyms

Synonyms	Comment	Organism
alpha-Gal	-	Homo sapiens
alpha-Gal A	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	defects in human alpha-GAL lead to the development of Fabry disease, a lysosomal storage disorder characterized by the buildup of alpha-galactosylated substrates in the tissues	Homo sapiens

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Release 2023.1 (January 2023)