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Literature summary for 3.2.1.22 extracted from
- Characterisation of an alpha-galactosidase with potential relevance to ripening related texture changes (2006), Phytochemistry, 67, 242-254.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 1.0 mM, complete inhibition, alpha-galactosidase 1; 1.0 mM, complete inhibition, alpha-galactosidase 2; 1.0 mM, complete inhibition, alpha-galactosidase 3 | Carica papaya |  |
| D-fucose | 75 mM, 11.1% inhibition, alpha-galactosidase 3; 75 mM, 25.5% inhibition, alpha-galactosidase 2; 75 mM, 47.1% inhibition, alpha-galactosidase 1 | Carica papaya | |
| D-galactose | 10 mM, 81.2% inhibition, alpha-galactosidase 1; 5 mM, 63% inhibition, alpha-galactosidase 2; 5 mM, 66.6% inhibition, alpha-galactosidase 3 | Carica papaya | |
| D-xylose | 75 mM, 45% inhibition, alpha-galactosidase 3; 75 mM, 62.7% inhibition, alpha-galactosidase 2; 75 mM, 81.5% inhibition, alpha-galactosidase 1 | Carica papaya | |
| HgCl2 | 0.5 mM, complete inhibition, alpha-galactosidase 1; 2.0 mM, complete inhibition, alpha-galactosidase 2; 2.0 mM, complete inhibition, alpha-galactosidase 3 | Carica papaya | |
| KCl | 2.0 M, 41.3% inhibition; 2.0 M, 74.6% inhibition, alpha-galactosidase 3; 2.0 M, 8.8% inhibition, alpha-galactosidase 1 | Carica papaya | |
| NaCl | 2.0 M, 21.9% inhibition, alpha-galactosidase 1; 2.0 M, 46.7% inhibition, alpha-galactosidase 2; 2.0 M, 78.9% inhibition, alpha-galactosidase 2 | Carica papaya | |
| p-nitrophenyl-alpha-D-galactoside | substrate inhibition above 4 mM, alpha-galactosidase 2; substrate inhibition above 4 mM, alpha-galactosidase 3 | Carica papaya | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.09 | - |
p-nitrophenyl-alpha-D-galactoside | alpha-galactosidase 3 | Carica papaya | |
| 1.35 | - |
p-nitrophenyl-alpha-D-galactoside | alpha-galactosidase 2 | Carica papaya | |
| 1.36 | - |
p-nitrophenyl-alpha-D-galactoside | alpha-galactosidase 1 | Carica papaya | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
alpha-galactosidase 3, gel filtration | Carica papaya |
| 52000 | - |
x * 52000, as SDS-PAGE analysis might overestimate the molecular size of glycoproteins it seems probable that the native alpha-galactosidase 2 is a tetramer, SDS-PAGE | Carica papaya |
| 57000 | - |
alpha-galactosidase 1, gel filtration | Carica papaya |
| 170000 | - |
alpha-galactosidase2, gel filtration | Carica papaya |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Carica papaya | alpha-galactosidase 2 has the ability to markedly catalyze increased pectin solubility and depolymerization while the polymers are still structurally attached to the cell walls mimicking, in part, the changes that occur during ripening. The enzyme might contribute to papaya fruit softening during ripening | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | alpha-galactosidase 2 | Carica papaya |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| alpha-galactosidase 1 | Carica papaya |
| alpha-galactosidase 2 | Carica papaya |
| alpha-galactosidase 3 | Carica papaya |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | alpha-galactosidase 1 | Carica papaya | - |
| fruit | alpha-galactosidase 3 | Carica papaya | - |
| fruit | protein level of alpha-galactosidase 2 is low in developing fruits and generally increased with ripening | Carica papaya | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.1 | - |
alpha-galactosidase 3 | Carica papaya |
| 14.46 | - |
alpha-galactosidase 1 | Carica papaya |
| 53.26 | - |
alpha-galactosidase 2 | Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinogalactan + H2O | alpha-galactosidase 1 | Carica papaya | ? | - |
? | |
| arabinogalactan + H2O | alpha-galactosidase 2 | Carica papaya | ? | - |
? | |
| arabinogalactan + H2O | alpha-galactosidase 3 | Carica papaya | ? | - |
? | |
| galactan + H2O | alpha-galactosidase 1 | Carica papaya | ? | - |
? | |
| galactan + H2O | alpha-galactosidase 2 | Carica papaya | ? | - |
? | |
| galactan + H2O | alpha-galactosidase 3 | Carica papaya | ? | - |
? | |
| additional information | alpha-galactosidase 2 has the ability to markedly catalyze increased pectin solubility and depolymerization while the polymers are still structurally attached to the cell walls mimicking, in part, the changes that occur during ripening. The enzyme might contribute to papaya fruit softening during ripening | Carica papaya | ? | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | alpha-galactosidase 1 | Carica papaya | p-nitrophenol + alpha-D-galactose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | alpha-galactosidase 2 | Carica papaya | p-nitrophenol + alpha-D-galactose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | alpha-galactosidase 3 | Carica papaya | p-nitrophenol + alpha-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, as SDS-PAGE analysis might overestimate the molecular size of glycoproteins it seems probable that the native alpha-galactosidase 2 is a tetramer, SDS-PAGE | Carica papaya |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-galactosidase 1 | - |
Carica papaya |
| alpha-galactosidase 2 | - |
Carica papaya |
| alpha-galactosidase 3 | - |
Carica papaya |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
alpha-galactosidase 3 | Carica papaya |
| 45 | - |
alpha-galactosidase 2 | Carica papaya |
| 65 | - |
alpha-galactosidase 1 | Carica papaya |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
10 min, 50% loss of activity, alpha-galactosidase 2 | Carica papaya |
| 48 | - |
10 min, 50% loss of activity, alpha-galactosidase 3 | Carica papaya |
| 55 | - |
10 min, 50% loss of activity, alpha-galactosidase 1 | Carica papaya |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
alpha-galactosidase 2 | Carica papaya |
| 3.5 | - |
alpha-galactosidase 3 | Carica papaya |
| 5.5 | - |
alpha-galactosidase 1 | Carica papaya |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Carica papaya | isoelectric focusing, alpha-galactosidase 2 | - |
7.2 |
| Carica papaya | isoelectric focusing, alpha-galactosidase 3 | - |
9.3 |
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Release 2023.1 (January 2023)
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