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Literature summary for 3.2.1.22 extracted from
- Characterization of a protease-resistant alpha-galactosidase from the thermophilic fungus Rhizomucor miehei and its application in removal of raffinose family oligosaccharides (2012), Biores. Technol., 110, 578-586 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme completely hydrolyzes raffinose and stachyose present in soybeans and kidney beans at 50°C within 60 min | Rhizomucor miehei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rhizomucor miehei |
General Stability
| General Stability | Organism |
|---|---|
| enzyme displays remarkable resistance to proteases | Rhizomucor miehei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 4% of initial activity | Rhizomucor miehei |  |
| Hg2+ | 8% of initial activity | Rhizomucor miehei | |
| additional information | not inhibitory: D-galactose at 100 mM, or dithiothreitol, EDTA | Rhizomucor miehei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 4.5, 50°C | Rhizomucor miehei | |
| 16.9 | - |
melibiose | pH 4.5, 50°C | Rhizomucor miehei | |
| 27.6 | - |
stachyose | pH 4.5, 50°C | Rhizomucor miehei | |
| 47.9 | - |
raffinose | pH 4.5, 50°C | Rhizomucor miehei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 129% of initial activity | Rhizomucor miehei | |
| Co2+ | 195% of initial activity | Rhizomucor miehei | |
| Cu2+ | 119% of initial activity | Rhizomucor miehei | |
| Fe2+ | 194% of initial activity | Rhizomucor miehei | |
| Zn2+ | 116% of initial activity | Rhizomucor miehei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 312000 | - |
gel filtration | Rhizomucor miehei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | H8Y263 | - |
- |
| Rhizomucor miehei CAU432 | H8Y263 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 198 | - |
substrate 4-nitrophenyl alpha-D-galactopyranoside, pH 4.5, 50°C | Rhizomucor miehei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Rhizomucor miehei CAU432 | 4-nitrophenol + alpha-D-galactose | - |
? | |
| melibiose + H2O | 5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei | alpha-D-galactose + alpha-D-glucose | - |
? | |
| melibiose + H2O | 5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei CAU432 | alpha-D-galactose + alpha-D-glucose | - |
? | |
| raffinose + H2O | 13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei | D-galactose + sucrose | - |
? | |
| raffinose + H2O | 13.5% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei CAU432 | D-galactose + sucrose | - |
? | |
| stachyose + H2O | 31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei | raffinose + D-galactose | - |
? | |
| stachyose + H2O | 31% of the activity with 4-nitrophenyl alpha-D-galactopyranoside | Rhizomucor miehei CAU432 | raffinose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 85141, calculated, 4 * 85000, SDS-PAGE | Rhizomucor miehei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gal36 | - |
Rhizomucor miehei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Rhizomucor miehei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30 min, 100% residual activity | Rhizomucor miehei |
| 60 | - |
30 min, 70% reidual activity | Rhizomucor miehei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
melibiose | pH 4.5, 50°C | Rhizomucor miehei | |
| 4.2 | - |
raffinose | pH 4.5, 50°C | Rhizomucor miehei | |
| 6.3 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 4.5, 50°C | Rhizomucor miehei | |
| 6.4 | - |
stachyose | pH 4.5, 50°C | Rhizomucor miehei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Rhizomucor miehei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | - |
Rhizomucor miehei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhizomucor miehei | isoelectric focusing | - |
5.8 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
melibiose | pH 4.5, 50°C | Rhizomucor miehei | |
| 0.09 | - |
raffinose | pH 4.5, 50°C | Rhizomucor miehei | |
| 0.23 | - |
stachyose | pH 4.5, 50°C | Rhizomucor miehei | |
| 17.5 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH 4.5, 50°C | Rhizomucor miehei | |
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