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(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
(S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine + ?
(S)-(cis-S-1-propenyl)thioglycine + L-glutamate
-
-
-
-
?
1-menaphthylglutathione + glycylglycine
?
-
-
-
-
?
2,4-dinitrophenylglutathione + glycylglycine
?
-
-
-
-
?
4,4'-diphenylmethane diisocyanate linked (Cys-Gly)-GSH + glycylglycine
4,4'-diphenylmethane diisocyanate linked (Cys-Gly)-(Cys-Gly) + L-Glu-Gly-Gly
-
-
-
-
?
4,4'-diphenylmethane diisocyanate linked GSH-GSH + glycylglycine
4,4'-diphenylmethane diisocyanate linked (Cys-Gly)-GSH + L-Glu-Gly-Gly
-
-
-
-
?
4-methylbiphenylylglutathione + glycylglycine
?
-
-
-
-
?
4-nitrobenzylglutathione + glycylglycine
?
-
-
-
-
?
5-D-Glu-4-nitroanilide + an amino acid
?
-
-
-
-
?
5-D-glutamyl 3-carboxy-4-nitroanilide + glycylglycine
5-D-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
5-D/L-glutamyl-phenylhydrazine + acceptor
phenylhydrazine + 5-L-glutamyl-acceptor
-
-
product is cytotoxic
ir
5-L-glutamine + 5-L-glutamine
5-poly-glutamic acid + glutamate
-
-
-
?
5-L-glutamyglycylglycine + acceptor
glycylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitroanilide + glucana
5-amino-2-nitrobenzoic acid + N-(3-carboxy-4-nitrophenyl)-L-glutaminyl-L-glutamic acid
-
-
-
ir
5-L-glutamyl 3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
5-L-glutamyl 4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
in the standard GGT assay with gamma-glutamyl p-nitroanilide as substrate, GGT1 accounts for 80% to 99% of the activity in all tissues except seeds where GGT2 is 50% of the activity
-
-
?
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
5-L-glutamyl-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 2-naphthylamine
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
5-L-glutamyl-4-methoxy-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 4-methoxy-2-naphthylamine
-
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-L-glutamyl-acceptor
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
5-L-glutamyl-4-nitroanilide + Asn
4-nitroaniline + 5-L-glutamyl-Asn
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
5-L-glutamyl-4-nitroanilide + DL-Phe
4-nitroaniline + 5-L-glutamyl-DL-Phe
-
39.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
N-ethyl-L-glutamine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
theanine + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
5-L-glutamyl-4-nitroanilide + glycyl-glycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycyl-L-proline
4-nitroaniline + 5-L-glutamyl-glycyl-L-proline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + H2O
p-nitroaniline + Glu
-
in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity
-
-
?
5-L-glutamyl-4-nitroanilide + His
4-nitroaniline + 5-L-glutamyl-His
-
82.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
5-L-glutamyl-4-nitroanilide + L-Ala
4-nitroaniline + 5-L-glutamyl-L-Ala
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
5-L-glutamyl-4-nitroanilide + L-alanylglycine
4-nitroaniline + 5-L-glutamyl-L-alanylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Arg
4-nitroaniline + 5-L-glutamyl-L-Arg
-
21.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
5-L-glutamyl-4-nitroanilide + L-Asn
4-nitroaniline + 5-L-glutamyl-L-Asn
-
24.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Asp
4-nitroaniline + 5-L-glutamyl-L-Asp
-
20.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
5-L-glutamyl-4-nitroanilide + L-Cys
4-nitroaniline + 5-L-glutamyl-L-Cys
-
11.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
5-L-glutamyl-4-nitroanilide + L-Gln
4-nitroaniline + 5-L-glutamyl-L-Gln
-
5.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Glu
4-nitroaniline + 5-L-glutamyl-L-Glu
-
8.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
5-L-glutamyl-4-nitroanilide + L-His
4-nitroaniline + 5-L-glutamyl-L-His
-
35.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
5-L-glutamyl-4-nitroanilide + L-Ile
4-nitroaniline + 5-L-glutamyl-L-Ile
-
19.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
5-L-glutamyl-4-nitroanilide + L-Leu
4-nitroaniline + 5-L-glutamyl-L-Leu
-
21.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
5-L-glutamyl-4-nitroanilide + L-leucyl-L-alanine
4-nitroaniline + 5-L-glutamyl-L-leucyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Lys
4-nitroaniline + 5-L-glutamyl-L-Lys
-
11.2% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
5-L-glutamyl-4-nitroanilide + L-Met
4-nitroaniline + 5-L-glutamyl-L-Met
5-L-glutamyl-4-nitroanilide + L-methioninamide
4-nitroaniline + 5-L-glutamyl-L-methioninamide
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
5-L-glutamyl-4-nitroanilide + L-methionyl(2,2,2-trifluoroethylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(2,2,2-trifluoroethylamide)
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(2-fluoroethylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(2-fluoroethylamide)
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(3,3,3-trifluoropropylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(3,3,3-trifluoropropylamide)
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionylethylamide
4-nitroaniline + 5-L-glutamyl-L-methionylethylamide
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionylpropylamide
4-nitroaniline + 5-L-glutamyl-L-methionylpropylamide
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Phe
4-nitroaniline + 5-L-glutamyl-L-Phe
-
38.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
5-L-glutamyl-4-nitroanilide + L-Pro
4-nitroaniline + 5-L-glutamyl-L-Pro
-
12.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
5-L-glutamyl-4-nitroanilide + L-Ser
4-nitroaniline + 5-L-glutamyl-L-Ser
-
12.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
5-L-glutamyl-4-nitroanilide + L-Thr
4-nitroaniline + 5-L-glutamyl-L-Thr
-
34% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
5-L-glutamyl-4-nitroanilide + L-Trp
4-nitroaniline + 5-L-glutamyl-L-Trp
-
30.9% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
5-L-glutamyl-4-nitroanilide + L-tyrosine
4-nitroaniline + 5-L-glutamyl-L-tyrosine
-
24.1% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Val
4-nitroaniline + 5-L-glutamyl-L-Val
-
30.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
5-L-glutamyl-4-nitroanilide + Leu
4-nitroaniline + 5-L-glutamyl-Leu
-
21.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Lys
4-nitroaniline + 5-L-glutamyl-Lys
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
5-L-glutamyl-4-nitroanilide + Pro
4-nitroaniline + 5-L-glutamyl-Pro
-
13.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + S-alkylcysteine sulfoxide
4-nitroaniline + 5-L-glutamyl-S-alkylcysteine sulfoxide
-
best substrates
-
-
?
5-L-glutamyl-4-nitroanilide + Ser
4-nitroaniline + 5-L-glutamyl-Ser
-
12.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + taurine
4-nitroaniline + 5-L-glutamyl-taurine
-
68.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Thr
4-nitroaniline + 5-L-glutamyl-Thr
5-L-glutamyl-4-nitroanilide + Tyr
4-nitroaniline + 5-L-glutamyl-Tyr
-
34.0% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Val
4-nitroaniline + 5-L-glutamyl-Val
-
30.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
5-L-glutamyl-cis-3-amino-L-proline + acceptor
5-L-glutamyl-acceptor + cis-3-amino-L-proline
-
-
-
?
5-L-glutamyl-L-alanine + 5-L-glutamyl-L-alanine
L-glutamine + 5-L-glutamyl-L-glutamyl-L-alanine
-
shows also hydrolase activity with this substrate
-
?
5-L-glutamyl-L-His + Gly-Gly
L-His + 5-L-glutamyl-Gly-Gly
-
106% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-leucine + glycylglycine
L-Leu + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-L-lysine + acceptor
L-lysine + 5-L-glutamyl-acceptor
-
shows also hydrolase activity with this substrate
-
?
5-L-glutamyl-L-Met + Gly-Gly
L-Met + 5-L-glutamyl-Gly-Gly
-
114% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Phe + Gly-Gly
L-Phe + 5-L-glutamyl-Gly-Gly
-
103% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Trp + Gly-Gly
L-Trp + 5-L-glutamyl-Gly-Gly
-
127% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Tyr + Gly-Gly
L-Tyr + 5-L-glutamyl-Gly-Gly
-
94.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Val + Gly-Gly
L-Val + 5-L-glutamyl-Gly-Gly
-
113% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamylaniline + 5-L-glutamylaniline
aniline + 5-L-glutamyl-5-L-glutamylaniline
-
-
-
ir
5-L-glutamylaniline + H2O
aniline + L-glutamic acid
-
-
-
ir
5-L-glutamylaniline + S-methyl-L-cysteine
aniline + 5-L-glutamyl-S-methyl-L-cysteine
-
-
-
ir
7-(N-gamma-glutamylamino)-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + ?
9-methylanthracenylglutathione + glycylglycine
?
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
a (5-L-glutamyl)-peptide + L-cysteinylglycine
?
-
-
-
-
?
D-gamma-glutamyl-S-([4-[(4-aminophenyl)methyl]phenyl]carbamoyl)-L-cysteinylglycine + glycylglycine
S-([4-[(4-aminophenyl)methyl]phenyl]carbamoyl)-L-cysteinylglycine + L-Glu-Gly-Gly
-
-
-
-
?
D-Gln + Ala
D-Glu-Ala + NH3
-
6.2% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Cys
D-Glu-Cys + NH3
-
19.3% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + ethylamine
?
1.3% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
D-Gln + Glu
D-Glu-Glu + NH3
-
8.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Gly
D-Glu-Gly + NH3
-
20.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Gly-Gly
Glu-Gly-Gly + NH3
-
-
-
-
?
D-Gln + His
D-Glu-His + NH3
-
116% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + L-Val
D-Glu-Val + NH3
-
8.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Met
D-Glu-Met + NH3
-
352% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Phe
D-Glu-Phe + NH3
-
144% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + taurine
D-Glu-taurine + NH3
-
422% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Trp
D-Glu-Trp + NH3
-
558% of the activity with D-Gln and Gly-Gly
-
-
?
diclofenac-S-acyl-glutathione + Gly
diclofenac-S-acyl-cysteinylglycine + ?
-
-
-
-
?
gamma-Glu-Cys + Gly-Gly
Cys + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
gamma-Glu-L-Tyr + ethylamine
?
5.2% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
gamma-Glu-Leu + ethylamine
?
3.4% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
gamma-glutamyl-4-nitroanilide + hydroxamate
4-nitroaniline + gamma-L-glutamylhydroxamate
gamma-glutamyl-D,L-phenylthioglycylglycine + acceptor
gamma-glutamyl-acceptor + alpha-phenylthioglycyl-glycine
-
-
-
-
?
gamma-glutamyl-S-[(6-[[([(2R)-2-amino-3-[(carboxymethyl)amino]-3-oxopropyl]sulfanyl)carbonyl]amino]hexyl)carbamoyl]cysteinylglycine + glycylglycine
(5R,20S)-5,20-diamino-4,8,17,21-tetraoxo-7,18-dithia-3,9,16,22-tetraazatetracosane-1,24-dioic acid + L-Glu-Gly-Gly
-
-
-
-
?
gamma-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + gamma-L-glutamyl-Gly-Gly
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine
L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine
glutamic acid gamma methyl ester + ethylamine
theanine + ethanol
-
-
-
-
ir
glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
glutathione + 1-aminocyclopropane-1-carboxylic acid
L-cysteinylglycine + 5-L-glutamyl-1-aminocyclopropane-1-carboxylic acid
-
isoforms I and II
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
glutathione + amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Ggt1 initiates enzymatic breakdown of GSH by cleavage of the gamma-glutamyl bond and release of cysteinylglycine. Second, the thiol group of the released dipeptide reduces ferric to ferrous iron. A combination of kinetic properties of both reactions results in efficient iron reduction over a broad pH range
-
-
?
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
glutathione + daunomycin
L-cysteinylglycine + 5-L-glutamyl-daunomycin
-
detoxification of daunomycin by transpeptidation
-
ir
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
glutathione + Gly-Gly
L-cysteinylglycine + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
glutathione + leukotriene D
cysteinylglycine + leukotriene C
glutathione disulfide + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
glutathione sulfonic acid + glycylglycine
?
-
-
-
?
glutathionesulfonic acid + glycylglycine
?
-
-
-
?
GS-bimane + Gly-Gly
?
-
GS-bimane is the conjugate of GSH and monobromobimane
-
-
?
GSH + ethylamine
?
56.7% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
GSH + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
-
-
?
GSH + L-glutamine
?
-
-
-
-
?
GSSG + ethylamine
?
10.6% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
GSSG + Gly-Gly
?
-
-
-
-
?
GSSG + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
-
-
?
hexane-1,6-diyldicarbamic acid linked GSH-GSH + glycylglycine
gamma-glutamyl-S-[(6-[[([(2R)-2-amino-3-[(carboxymethyl)amino]-3-oxopropyl]sulfanyl)carbonyl]amino]hexyl)carbamoyl]cysteinylglycine + L-Glu-Gly-Gly
-
-
-
-
?
hypoglycin A + GSH
hypoglycin B + cysteinylglycine
-
-
-
-
?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor
L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
L-gamma-glutamyl-3-carboxy-4-nitroanilide + H2O
3-carboxy-4-nitroaniline + L-glutamate
assay at pH 8
-
-
?
L-gamma-glutamyl-4-nitroanilide + ethylamine
4-nitroaniline + 5-L-glutamyl-ethylamine
L-gamma-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
L-gamma-glutamyl-4-nitroanilide + glycine methylester
4-nitroaniline + 5-L-glutamyl-glycyl methylester
very low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
L-gamma-glutamyl-4-nitroanilide + glycylglycine methylester
4-nitroaniline + 5-L-glutamyl-glycylglycine methylester
low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
L-gamma-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
L-gamma-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
L-gamma-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
L-gamma-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
very low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
L-gamma-glutamyl-S-[(6-aminohexyl)carbamoyl]-D-cysteinylglycine + glycylglycine
[[(2R)-2-amino-3-[[(6-aminohexyl)carbamoyl]sulfanyl]propanoyl]amino]acetic acid + L-Glu-Gly-Gly
-
-
-
-
?
L-Gln + ethylamine
?
18.8% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Gln + Gly-Gly
NH3 + 5-L-glutamyl-Gly-Gly
-
78.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
L-Gln + taurine
gamma-glutamyltaurine + NH3
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
L-Glu-gamma-3-carboxy-4-hydroxy-anilide + ethylamine
?
3.9% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monohydroxamate + ethylamine
?
0.5% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monomethyl ester + ethylamine
?
L-glutamic acid 5-(3-carboxy-4-nitroanilide) + ?
?
-
-
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
L-glutamine + glycylglycine
NH3 + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-glutamine + L-leucine
gamma-glutamyl-L-leucine + NH3
-
optimum conditions for a biocatalytic synthesis of gamma-Glu-Leu are determined to be 200 mM glutamine, 200 mM leucine, 50 mM Tris-HCl, pH 9.0, and enzyme BlGGT at a working concentration of 2.0 U/ml. Upon a 5-h incubation of the reaction mixture at 50ưC, the product yield reaches approximately 52%. The preparative synthesis of gamma-Glu-Leu by BlGGT is done under optimal conditions at millimolar scale level
-
-
?
L-glutamyl-gamma-7-amido-4-methylcoumarin + glycyclglycine
7-amino-4-methylcoumarin + L-glutamyl-glycylglycine
-
-
-
-
?
L-glutamylhydrazine + methylamine
gamma-L-glutamylmethylamide + hydrazine
recombinant enzyme, method optimmization and evaluation, overview
-
-
?
lentinic acid + amino acid
desglutamyl lentinic acid + 5-L-glutamylamino acid
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
leukotriene C4 + glycylglycine
leukotriene D4 + 5-L-glutamyl-glycylglycine
-
-
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
S-(4-nitro-benzyl)glutathione + glycylglycine
?
-
-
-
?
S-methyl-glutathione + ethylamine
?
1.8% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
S-methylglutathione + acceptor
S-methyl-L-cysteinylglycine + 5-L-glutamyl-acceptor
-
shows also hydrolase activity with this substrate
-
?
S-methylglutathione + glycylglycine
?
-
-
-
?
S-nitroso-glutathione + glycyl-glycine
S-nitroso-cysteinylglycine + gamma-L-glutamyl-glycyl-glycine
-
-
-
-
?
additional information
?
-
(5-L-glutamyl)-peptide + acceptor + H+

peptide + 5-L-glutamyl amino acid
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors are Glu, alpha-L-Glu-L-Ala
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
dipeptides are better acceptors than free amino acids
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
isozyme A prefers basic and aromatic amino acids as acceptors
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetylglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors are Glu, alpha-L-Glu-L-Ala
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
Oncornavirus
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
487899, 487905, 487912, 487914, 487915, 487917, 487918, 487919, 487920, 487957, 487958, 487960, 487964, 487969 -
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
poor substrates are beta-substituted amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
imino acids are no substrates
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: leukotriene C
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione S-conjugates
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
alpha-aminoisobutyric acid is no donor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors: L-methionine, L-tryptophan, L-alanine, 1-aminocyclopropane-1-carboxylic acid, glutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors: 5-ethyl-Glu and beta-benzyl-Glu, hydrolyzed by pig enzyme
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: leukotriene C
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
L-cysteine is no substrate
-
?
(5-L-glutamyl)-peptide + an amino acid

peptide + 5-L-Glu-Xaa
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
(5-L-glutamyl)-peptide + an amino acid

peptide + a 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine

4-nitroaniline + gamma-glutamylglycylglycine
quantitative measurement of release of 4-nitroaniline
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
-
the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-p-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
quantitative measurement of release of 4-nitroaniline
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
chromogenic gamma-glutamyl-4-nitroanilide (GPNA) reacts as the donor substrate affording the gamma-glutamyl-enzyme intermediate through reaction with the catalytically active threonine residue at the N-terminus of the small subunit of the enzyme. In this step 4-nitroaniline (PNA) is liberated, which can be spectrophotometrically detected at 410 nm. The gamma-glutamyl-enzyme intermediate is then resolved by nucleophilic attack of the free amino group of glycylglycine, present in solution in excess amount. The transpeptidation product gamma-glutamylglycylglycine is formed and the enzyme is restored in its free state, able to start a new catalytic cycle. The reaction of the gamma-glutamyl enzyme intermediate with a nucleophile is the rate-determining step of the process, thus the rate of liberation of PNA is usually considered a measure of the rate of the transpeptidase activity
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
chromogenic gamma-glutamyl-4-nitroanilide (GPNA) reacts as the donor substrate affording the gamma-glutamyl-enzyme intermediate through reaction with the catalytically active threonine residue at the N-terminus of the small subunit of the enzyme. In this step 4-nitroaniline (PNA) is liberated, which can be spectrophotometrically detected at 410 nm. The gamma-glutamyl-enzyme intermediate is then resolved by nucleophilic attack of the free amino group of glycylglycine, present in solution in excess amount. The transpeptidation product gamma-glutamylglycylglycine is formed and the enzyme is restored in its free state, able to start a new catalytic cycle. The reaction of the gamma-glutamyl enzyme intermediate with a nucleophile is the rate-determining step of the process, thus the rate of liberation of PNA is usually considered a measure of the rate of the transpeptidase activity
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor

4-nitroaniline + 5-D-glutamyl-acceptor
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine

5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
-
-
-
ir
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine

3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-2-naphthylamide + glycylglycine

5-L-glutamyl-glycylglycine + 2-naphthylamine
-
-
-
?
5-L-glutamyl-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 2-naphthylamine
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide

5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine

5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate

4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
beta-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
alpha-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
alpha-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid

4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
161% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
176% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
8% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
8% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide

5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
autotranspeptidase
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
autotranspeptidation, L- or, to a lesser extent, D-enantiomer
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala

4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg

4-nitroaniline + 5-L-glutamyl-Arg
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
113.6% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
113.6% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
124% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
169% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
44% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
44% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Asn

4-nitroaniline + 5-L-glutamyl-Asn
-
39.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asn
4-nitroaniline + 5-L-glutamyl-Asn
-
39.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp

4-nitroaniline + 5-L-glutamyl-Asp
-
4.5% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
4.5% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
13% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
13% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
140% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
144% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + citrulline

4-nitroaniline + 5-L-glutamyl-citrulline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Cys

4-nitroaniline + 5-L-glutamyl-Cys
-
46.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly

4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
15% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
16% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
36% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
36% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine

theanine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
theanine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Glu

4-nitroaniline + 5-L-glutamyl-Glu
-
4.4% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
11% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
13% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
13% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
13% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Gly

4-nitroaniline + 5-L-glutamyl-Gly
-
37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
33.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
33.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
14% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
14% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
43% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
48% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly

4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala

4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly

4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine

4-nitroaniline + 5-L-glutamyl-glycine
-
19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
Oncornavirus
-
19% viral enzyme
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
glutamyl transfer at 17.2%
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine

4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine

4-nitroaniline + 5-L-glutamylglycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
ir
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
GGT activity
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
Oncornavirus
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
4-nitroaniline is cytotoxic
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine

5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
substrate for isoform GGT1
-
-
?
5-L-glutamyl-4-nitroanilide + H2O

4-nitroaniline + Glu
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
ping-pong mechanism
-
-
?
5-L-glutamyl-4-nitroanilide + H2O

4-nitroaniline + L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
hydrolase reaction
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
hydrolase reaction
-
ir
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
hydrolase reaction
-
?
5-L-glutamyl-4-nitroanilide + H2O

L-glutamate + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ile

4-nitroaniline + 5-L-glutamyl-Ile
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
-
20.7% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ala

4-nitroaniline + 5-L-glutamyl-L-Ala
-
9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ala
4-nitroaniline + 5-L-glutamyl-L-Ala
-
9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine

4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
L-Ala or D-Ala
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
glutamyl transfer at 32% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine

4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
glutamyl transfer at 30% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine

4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate

4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
glutamyl transfer at 8.6% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine

4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine

4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine

4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
best acceptor, low molecular weight enzyme variant
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate

4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine

4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
best acceptor, high molecular weight enzyme variant
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
best acceptor
-
-
?