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Literature summary for 2.3.2.2 extracted from

  • Hussein, A.S.; Walter, R.D.
    Purification and characterization of gamma-glutamyl transpeptidase from Ascaris suum (1996), Mol. Biochem. Parasitol., 77, 41-47.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acivicin
-
Ascaris suum
D-serine
-
Ascaris suum
L- or D-Serine/borate competitive Ascaris suum
L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazolacetic acid i.e. acivicin; in vivo inhibition and accumulation of glutathione; kinetics; strong, irreversible, reacts with glutamyl binding site Ascaris suum
L-serine
-
Ascaris suum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Ascaris suum
additional information
-
additional information Km values for several amino acids and dipeptides, overview Ascaris suum
0.03
-
5-L-glutamyl-7-amido-4-methylcoumarin
-
Ascaris suum
1.28
-
glycylglycine
-
Ascaris suum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Ascaris suum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
1 * 43000 + 1 * 30000, SDS-PAGE Ascaris suum
43000
-
1 * 43000 + 1 * 30000, SDS-PAGE Ascaris suum
70000
-
gel filtration Ascaris suum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ Ascaris suum
-
peptide + 5-L-glutamyl amino acid
-
?
glutathione + an amino acid Ascaris suum initial step of glutathione degradation L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
?

Organism

Organism UniProt Comment Textmining
Ascaris suum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilized by Triton X-100 Ascaris suum

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Ascaris suum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities with diverse substrates Ascaris suum
1009
-
purified enzyme Ascaris suum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
Ascaris suum peptide + 5-L-glutamyl amino acid
-
?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ascaris suum peptide + 5-L-glutamyl amino acid
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor acceptors are amino acids and peptides, overview Ascaris suum 7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor glycylglycine is best acceptor Ascaris suum 7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
?
glutathione + an amino acid initial step of glutathione degradation Ascaris suum L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
?
L-glutamyl-gamma-7-amido-4-methylcoumarin + glycylglycine
-
Ascaris suum 7-amino-4-methylcoumarin + L-glutamyl-glycylglycine
-
?

Subunits

Subunits Comment Organism
dimer 1 * 43000 + 1 * 30000, SDS-PAGE Ascaris suum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ascaris suum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Ascaris suum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
L-serine in presence of borate Ascaris suum
0.42
-
acivicin
-
Ascaris suum
0.61
-
D-serine in presence of borate Ascaris suum