Application | Comment | Organism |
---|---|---|
diagnostics | direct detection of GGT activity can provide critical information for the diagnosis of several pathologies. Elevated serum GGT levels are a general marker of diseases affecting the liver | Rattus norvegicus |
medicine | direct detection of GGT activity can provide critical information for the diagnosis of several pathologies. GGT may serve as a tumor biomarker and its presence on the cell surface is particularly attractive for imaging applications | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acivicin | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Rattus norvegicus | 9986 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid | Rattus norvegicus | - |
a peptide + a 5-L-glutamyl amino acid | - |
? | |
additional information | Rattus norvegicus | in vivo expreriments in rat kidney, liver, heart, or brain. gamma-Glu-[1-13C]Gly is hyperpolarized using the TEMPOL nitroxyl radical (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), and the resulting 13C polarization at the time of injection is 9%, overview. A spectral peak corresponding to [1-13C]Gly is readily detected in the kidney | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
leukocyte | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
additional information | the enzyme is widely expressed on the apical surface of epithelial-cell lining ducts, most prominently in the proximal tubules of the kidney and liver biliary ducts, and is also expressed on leukocytes and astrocytes. Non-invasive, real-time detection of GGT activity in vivo, overview | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid | - |
Rattus norvegicus | a peptide + a 5-L-glutamyl amino acid | - |
? | |
additional information | in vivo expreriments in rat kidney, liver, heart, or brain. gamma-Glu-[1-13C]Gly is hyperpolarized using the TEMPOL nitroxyl radical (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), and the resulting 13C polarization at the time of injection is 9%, overview. A spectral peak corresponding to [1-13C]Gly is readily detected in the kidney | Rattus norvegicus | ? | - |
- |
|
additional information | enzyme GGT catalyzes the cleavage of the gamma-peptide bond between the glutamyl and cysteinyl residues of GSH and related molecules, either hydrolyzing the bond or ligating the free alpha-amine of an amino acid or peptide, cf. glutathione hydrolase, EC 3.4.19.13. Development of a HP-GGT probe for hyperpolarized (HP) MR measurements for dissolution dynamic nuclear polarization (DNP), a versatile method to hyperpolarize nuclear spins. It can temporarily increase the signal from NMR-active nuclei by as much as 4 or 5 orders of magnitude, which has enabled the detection of metabolic transformations in real-time with high sensitivity using NMR spectroscopy and imaging. Design and preparation of an HP molecular probe targeting GGT activity, gamma-Glu-[1-13C]Gly. gamma-Glu-[1-13C]Gly fulfills the requirements of an HP molecular probe, and the probe can monitor real-time GGT activity in vivo. Detection of enzyme bound-gamma-Glu and radiolabeled Gly from hydrolysis. Method, overview | Rattus norvegicus | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl transpeptidase | - |
Rattus norvegicus |
GGT | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the gamma-glutamyl transpeptidase (GGT) is a cell surface enzyme that plays an essential central role in glutathione (GSH) homeostasis. Elevated serum GGT levels are a general marker of diseases affecting the liver | Rattus norvegicus |