Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Raphanus sativus | |
acivicin | - |
Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
0.032 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
0.038 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
0.038 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
0.042 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
0.042 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
0.046 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
0.046 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
0.21 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
0.22 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
0.39 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
0.41 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
0.44 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
0.47 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
0.6 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
0.66 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
2.3 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
2.4 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
4.3 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
4.4 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | bound to, enzyme form GGT A | Raphanus sativus | 5618 | - |
cell wall | bound to, enzyme form GGT B | Raphanus sativus | 5618 | - |
soluble | enzyme form GGT I | Raphanus sativus | - |
- |
soluble | enzyme form GGT II | Raphanus sativus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE | Raphanus sativus |
21000 | - |
1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE | Raphanus sativus |
42000 | - |
1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE | Raphanus sativus |
42000 | - |
1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE | Raphanus sativus |
61000 | - |
enzyme form GGT A, gel filtration | Raphanus sativus |
61000 | - |
enzyme form GGT B, gel filtration | Raphanus sativus |
63000 | - |
enzyme form GGT I, gel filtration | Raphanus sativus |
63000 | - |
enzyme form GGT II, gel filtration | Raphanus sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme form GGT A | Raphanus sativus |
glycoprotein | enzyme form GGT B | Raphanus sativus |
glycoprotein | enzyme form GGT I | Raphanus sativus |
glycoprotein | enzyme form GGT II | Raphanus sativus |
Purification (Comment) | Organism |
---|---|
enzyme form GGT A | Raphanus sativus |
enzyme form GGT B | Raphanus sativus |
enzyme form GGT I | Raphanus sativus |
enzyme form GGT II | Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Raphanus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
enzyme form GGT I | Raphanus sativus |
0.59 | - |
enzyme form GGT II | Raphanus sativus |
1.48 | - |
enzyme form GGT A | Raphanus sativus |
1.73 | - |
enzyme form GGT B | Raphanus sativus |
Storage Stability | Organism |
---|---|
-20°C or 4°C, activity of enzyme form GGT A is lost within 1 week | Raphanus sativus |
-20°C or 4°C, activity of enzyme form GGT B is lost within 1 week | Raphanus sativus |
-80°C, stable, enzyme form GGT I | Raphanus sativus |
-80°C, stable, enzyme form GGT II | Raphanus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid | 161% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid | - |
? | |
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid | 176% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid | - |
? | |
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid | 8% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid | - |
? | |
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid | 8% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | 124% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | 169% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | 44% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Arg | 44% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Arg | - |
? | |
5-L-glutamyl-4-nitroanilide + Asp | 13% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + Asp | 13% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + Asp | 140% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + Asp | 144% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Asp | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys | 7% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys | 7% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys | 7% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys | 7% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys-Gly | 15% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys-Gly | 16% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys-Gly | 36% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Cys-Gly | 36% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Cys-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Glu | 11% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + Glu | 13% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + Glu | 13% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + Glu | 13% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Glu | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 14% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 14% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 43% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly | 48% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + Met | 137% of the activity with Gly-Gly, enzyme form GGT II | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Met | - |
? | |
5-L-glutamyl-4-nitroanilide + Met | 141% of the activity with Gly-Gly, enzyme form GGT I | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Met | - |
? | |
5-L-glutamyl-4-nitroanilide + Met | 43% of the activity with Gly-Gly, enzyme form GGT A | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Met | - |
? | |
5-L-glutamyl-4-nitroanilide + Met | 43% of the activity with Gly-Gly, enzyme form GGT B | Raphanus sativus | 4-nitroaniline + 5-L-glutamyl-Met | - |
? | |
gamma-Glu-Cys + Gly-Gly | - |
Raphanus sativus | Cys + 5-L-glutamyl-Gly-Gly | - |
? | |
GS-bimane + Gly-Gly | GS-bimane is the conjugate of GSH and monobromobimane | Raphanus sativus | ? | - |
? | |
GSH + Gly-Gly | - |
Raphanus sativus | ? | - |
? | |
GSSG + Gly-Gly | - |
Raphanus sativus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 42000 + 1 * 21000, enzyme form GGT I, SDS-PAGE | Raphanus sativus |
dimer | 1 * 42000 + 1 * 21000, enzyme form GGT II, SDS-PAGE | Raphanus sativus |
monomer | 1 * 61000, enzyme form GGT A, SDS-PAGE | Raphanus sativus |
monomer | 1 * 61000, enzyme form GGT B, SDS-PAGE | Raphanus sativus |
Synonyms | Comment | Organism |
---|---|---|
GGT A | - |
Raphanus sativus |
GGT I | - |
Raphanus sativus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
12 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
13 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
13 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
15 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
16 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
20 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
21 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
23 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
27 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
30 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
30 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
31 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
31 | - |
gamma-Glu-Cys | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
32 | - |
GSH | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
32 | - |
GSSG | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus | |
33 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I | Raphanus sativus | |
38 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B | Raphanus sativus | |
39 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A | Raphanus sativus | |
40 | - |
GS-bimane | 37°C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
enzyme form GGT I | Raphanus sativus |
7.5 | - |
enzyme form GGT II | Raphanus sativus |
7.5 | 8 | enzyme form GGT A | Raphanus sativus |
7.5 | 8 | enzyme form GGT B | Raphanus sativus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT A | Raphanus sativus |
6 | 9 | pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT B | Raphanus sativus |
7 | 9 | pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT I | Raphanus sativus |
7 | 9 | pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT II | Raphanus sativus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
acivicin | 37°C, pH 8.0, enzyme form GGT II | Raphanus sativus | |
0.017 | - |
acivicin | 37°C, pH 8.0, enzyme form GGT I | Raphanus sativus | |
0.081 | - |
6-diazo-5-oxo-L-norleucine | 37°C, pH 8.0, enzyme form GGT I | Raphanus sativus | |
0.085 | - |
6-diazo-5-oxo-L-norleucine | 37°C, pH 8.0, enzyme form GGT II | Raphanus sativus | |
0.096 | - |
acivicin | 37°C, pH 8.0, enzyme form GGT A | Raphanus sativus | |
0.103 | - |
acivicin | 37°C, pH 8.0, enzyme form GGT B | Raphanus sativus | |
0.47 | - |
6-diazo-5-oxo-L-norleucine | 37°C, pH 8.0, enzyme form GGT B | Raphanus sativus | |
0.57 | - |
6-diazo-5-oxo-L-norleucine | 37°C, pH 8.0, enzyme form GGT A | Raphanus sativus |