KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics with L-gamma-glutamyl-4-nitroanilide as substrate, native extracellular enzyme | Aspergillus nidulans | |
0.2 | - |
L-gamma-glutamyl-4-nitroanilide | pH 8.0, 37°C, native extracellular enzyme | Aspergillus nidulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus nidulans | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid | Aspergillus nidulans | - |
a peptide + a 5-L-glutamyl amino acid | - |
? | |
a (5-L-glutamyl)-peptide + an amino acid | Aspergillus nidulans FGSC A26 | - |
a peptide + a 5-L-glutamyl amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme partially from carbon stressed cultures | Aspergillus nidulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | carbon stressed cultures | Aspergillus nidulans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (5-L-glutamyl)-peptide + an amino acid | - |
Aspergillus nidulans | a peptide + a 5-L-glutamyl amino acid | - |
? | |
a (5-L-glutamyl)-peptide + an amino acid | - |
Aspergillus nidulans FGSC A26 | a peptide + a 5-L-glutamyl amino acid | - |
? | |
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine | GSH | Aspergillus nidulans | L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine | - |
? | |
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine | GSH | Aspergillus nidulans FGSC A26 | L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine | - |
? | |
glutathione disulfide + glycylglycine | GSSG | Aspergillus nidulans | L-Cys-Gly + 5-L-glutamyl-glycylglycine | - |
? | |
glutathione disulfide + glycylglycine | GSSG | Aspergillus nidulans FGSC A26 | L-Cys-Gly + 5-L-glutamyl-glycylglycine | - |
? | |
L-gamma-glutamyl-4-nitroanilide + glycylglycine | - |
Aspergillus nidulans | 4-nitroaniline + 5-L-glutamyl-glycyl-glycine | - |
? | |
L-gamma-glutamyl-4-nitroanilide + glycylglycine | - |
Aspergillus nidulans FGSC A26 | 4-nitroaniline + 5-L-glutamyl-glycyl-glycine | - |
? | |
L-glutamine + glycylglycine | - |
Aspergillus nidulans | NH3 + 5-L-glutamyl-glycylglycine | - |
? | |
additional information | the enzyme utilizes Gln, glutathione, and less efficiently oxidized glutathione as gamma-glutamyl donors (beside of gamma-glutamyl-4-nitroanilide) and utilizes amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly), but not hydroxylamine, as gamma-glutamyl acceptors | Aspergillus nidulans | ? | - |
- |
|
additional information | the enzyme utilizes Gln, glutathione, and less efficiently oxidized glutathione as gamma-glutamyl donors (beside of gamma-glutamyl-4-nitroanilide) and utilizes amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly), but not hydroxylamine, as gamma-glutamyl acceptors | Aspergillus nidulans FGSC A26 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
AngammaGT | - |
Aspergillus nidulans |
gamma-glutamyl transpeptidase | - |
Aspergillus nidulans |
gammaGT | - |
Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aspergillus nidulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | no significant activities are detected below pH 7.0 or above pH 10.0 | Aspergillus nidulans |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the ggtA (AN10444) encoding a putative gammaGT decreased the gammaGT activities below to the detection limit suggesting that GgtA is the only enzyme possessing significant gammaGT activity under carbon stressed conditions in Aspergillus nidulans | Aspergillus nidulans |
additional information | enzyme AngammaGT has little hydrolase activity (cf. EC 3.4.19.13) | Aspergillus nidulans |
physiological function | the function of this enzyme is not to degrade, but to produce, gamma-glutamyl compounds, which may be related to the utilization of extracellular peptides and amino-acids in carbon stressed cultures | Aspergillus nidulans |