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Literature summary for 2.3.2.2 extracted from
- Purification and characterization of gamma-glutamyltransferase from rat pancreas (1982), Biochim. Biophys. Acta, 707, 66-73.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Iodoacetamidofluorescein | - |
Rattus norvegicus |  |
| 6-diazo-5-oxo-L-norleucine | irreversible inactivation of pancreatic enzyme, S-methylglutathione prevents at 10 mM, maleate increases inhibition at 0.25-1 M | Rattus norvegicus | |
| Antiserum to rat kidney glutamyltransferase | - |
Rattus norvegicus | |
| L- or D-Serine/borate | reversible | Rattus norvegicus | |
| L-azaserine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
L-Glu-4-nitroanilide | - |
Rattus norvegicus | |
| 20 | - |
glycylglycine | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Rattus norvegicus |
| 25000 | - |
1 * 43000 + 1 * 25000, SDS-PAGE | Rattus norvegicus |
| 43000 | - |
1 * 43000 + 1 * 25000, SDS-PAGE | Rattus norvegicus |
| 68000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar | - |
| Rattus norvegicus Wistar | - |
Wistar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 630 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5-L-glutamyl)-peptide + acceptor + H+ | - |
Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
| (5-L-glutamyl)-peptide + acceptor + H+ | - |
Rattus norvegicus Wistar | peptide + 5-L-glutamyl amino acid | - |
? | |
| 5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide | - |
Rattus norvegicus | 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline | - |
? | |
| 5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide | - |
Rattus norvegicus Wistar | 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline | - |
? | |
| 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
| 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Rattus norvegicus Wistar | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
| L-Glu-4-nitroanilide + glutathione | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? | |
| L-Glu-4-nitroanilide + glutathione | - |
Rattus norvegicus Wistar | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 43000 + 1 * 25000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
6-diazo-5-oxo-L-norleucine | - |
Rattus norvegicus | |
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