Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
T380A | mutant enzyme can not process even after 30 days at 37 °C, no enzymatic activity | Helicobacter pylori |
T380S | mutant enzyme is strongly impaired with respect to processing. Prolonged incubation (more than 30 days) at 37°C leads to complete maturation of the T380S mutant. Vmax for hydrolysis of 5-L-glutamyl-4-nitroanilide is 8.8fold lower than wild-type enzyme. Vmax for transpeptidation of 5-L-glutamyl-4-nitroanilide of Gly-Gly | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, transpeptidase reaction with Gly-Gly | Helicobacter pylori | |
0.0125 | - |
5-L-glutamyl-4-nitroanilide | 37°C, pH 8.0, hydrolysis | Helicobacter pylori | |
2.5 | - |
Gly-Gly | 37°C, pH 8.0 | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000 + 2 * 40000, SDS-PAGE | Helicobacter pylori |
40000 | - |
2 * 20000 + 2 * 40000, SDS-PAGE | Helicobacter pylori |
120000 | - |
gel filtration | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the 60000 Da proenzyme is kinetically competent to form the mature 40000 and 20000 Da subunits and exhibits maximal autoprocessing activity at neutral pH. Processing is required for enzymatic activity | Helicobacter pylori |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Helicobacter pylori |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.59 | - |
hydrolysis of glutathione | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Helicobacter pylori | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | - |
Helicobacter pylori | 4-nitroaniline + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 20000 + 2 * 40000, SDS-PAGE | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | hydrolysis of 5-L-glutamyl-4-nitroanilide | Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 40% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of 5-L-glutamyl-4-nitroanilide | Helicobacter pylori |