Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
N-terminal sequence | Bacillus subtilis |
22000 | - |
1 * 45000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
45000 | - |
1 * 45000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
58000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + an amino acid | Bacillus subtilis | involved in polyglutamic acid synthesis | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
natto strain NR-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities with diverse substrates | Bacillus subtilis |
62.9 | - |
purified enzyme | Bacillus subtilis |
81.5 | - |
purified enzyme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: hydrolase reaction with H2O as acceptor | Bacillus subtilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donors are Glu, alpha-L-Glu-L-Ala | Bacillus subtilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | dipeptides are better acceptors than free amino acids | Bacillus subtilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: autotranspeptidation with another donor molecule as acceptor | Bacillus subtilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Bacillus subtilis | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor specificity, overview | Bacillus subtilis | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamine + 5-L-glutamine | - |
Bacillus subtilis | 5-poly-glutamic acid + glutamate | - |
? | |
5-L-glutamyglycylglycine + acceptor | - |
Bacillus subtilis | glycylglycine + 5-L-glutamyl-acceptor | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Bacillus subtilis | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
glutathione + an amino acid | involved in polyglutamic acid synthesis | Bacillus subtilis | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 45000 + 1 * 22000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
60 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 80 | about half-maximal activity at 45°C and about 70% of maximal activity at 80°C | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at least 15 min stable | Bacillus subtilis |
55 | - |
inactivation within 15 min at pH 8.0 | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | about half-maximal activity at pH 7.5, about 90% of maximal activity at pH 9.0 | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | stable | Bacillus subtilis |