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Literature summary for 2.3.2.2 extracted from

  • Tomita, K.; Yano, T.; Tsuchida, T.; Kumagai, H.; Tochikura, T.
    Purification and properties of gamma-glutamyltranspeptidase from Penicillium roqueforti IFO 4622 (1990), J. Ferment. Bioeng., 70, 128-130.
No PubMed abstract available
Search for more literature for 2.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
Pb2+
-
 Penicillium roqueforti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005 0.0068 L-Glu-4-nitroanilide hydrolase reaction Penicillium roqueforti
0.0068
-
 L-Glu-4-nitroanilide hydrolase reaction Penicillium roqueforti
0.13 0.18 L-Glu-4-nitroanilide
-
 Penicillium roqueforti
48
-
 glycylglycine
-
 Penicillium roqueforti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 66000 + 1 * 55000, SDS-PAGE Penicillium roqueforti
66000
-
 1 * 66000 + 1 * 55000, SDS-PAGE Penicillium roqueforti
120000
-
 gel filtration Penicillium roqueforti

Organism

Organism UniProt Comment Textmining
Penicillium roqueforti
-
-
-
Penicillium roqueforti IFO 4622
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium roqueforti

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Penicillium roqueforti
-
Koji culture solid culture on wheat bran Penicillium roqueforti
-
mycelium
-
 Penicillium roqueforti
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.9
-
 purified enzyme Penicillium roqueforti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Penicillium roqueforti peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Penicillium roqueforti peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Penicillium roqueforti peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Penicillium roqueforti IFO 4622 peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Penicillium roqueforti IFO 4622 peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Penicillium roqueforti IFO 4622 peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Penicillium roqueforti IFO 4622 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-alanine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-arginine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-arginine
-
 ?
5-L-glutamyl-4-nitroanilide + L-asparagine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-asparagine
-
 ?
5-L-glutamyl-4-nitroanilide + L-aspartate
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-aspartate
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-cysteine
-
 ?
5-L-glutamyl-4-nitroanilide + L-cystine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-cystine
-
 ?
5-L-glutamyl-4-nitroanilide + L-cystine
-
 Penicillium roqueforti IFO 4622 4-nitroaniline + 5-L-glutamyl-L-cystine
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
5-L-glutamyl-4-nitroanilide + L-histidine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-histidine
-
 ?
5-L-glutamyl-4-nitroanilide + L-isoleucine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
5-L-glutamyl-4-nitroanilide + L-leucine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-leucine
-
 ?
5-L-glutamyl-4-nitroanilide + L-lysine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-serine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
5-L-glutamyl-4-nitroanilide + L-tryptophan
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
5-L-glutamyl-4-nitroanilide + L-valine
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Penicillium roqueforti 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 66000 + 1 * 55000, SDS-PAGE Penicillium roqueforti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Penicillium roqueforti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 30 at least 10 min stable Penicillium roqueforti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Penicillium roqueforti

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable Penicillium roqueforti