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Literature summary for 2.3.2.2 extracted from
- Improved catalytic efficiency of a monomeric gamma-glutamyl transpeptidase from Bacillus licheniformis in presence of subtilisin (2010), Biotechnol. Lett., 32, 1137-1141.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Subtilisin | subtilisin enhances transpeptidase activity of the 30000 Da and 67000 Da enzyme forms by nearly 1.5 and 2fold, respectively | Bacillus licheniformis |
General Stability
| General Stability | Organism |
|---|---|
| transpeptidase activity is salt-sensitive | Bacillus licheniformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
5-L-glutamyl-4-nitroanilide | monomeric 30-kDa enzyme, at pH 11.0, in the presence of subtilisin, at 80°C | Bacillus licheniformis |  |
| 0.25 | - |
5-L-glutamyl-4-nitroanilide | heterodimeric 67-kDa enzyme, at pH 8.0, in the absence of subtilisin, at 60°C | Bacillus licheniformis | |
| 5.2 | - |
5-L-glutamyl-4-nitroanilide | monomeric 30-kDa enzyme, at pH 8.0, in the absence of subtilisin, at 60°C | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
one of two active enzyme forms, gel filtration | Bacillus licheniformis |
| 67000 | - |
one of two active enzyme forms, gel filtration | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis ER-15 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography and G-75 Sephadex gel filtration | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Bacillus licheniformis | 5-L-glutamyl-glycylglycine + 4-nitroaniline | - |
? | |
| 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Bacillus licheniformis ER-15 | 5-L-glutamyl-glycylglycine + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer or heterodimer | two active enzyme forms of 30000 Da and 67000 Da | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-glutamyl transpeptidase | - |
Bacillus licheniformis |
| GGT | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
monomeric 30-kDa enzyme and heterodimeric 67000-Da enzyme, in the absence of subtilisin | Bacillus licheniformis |
| 70 | - |
monomeric 30-kDa enzyme, in presence of subtilisin | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the monomeric 30-kDa enzyme has a half-life of 240 min and 120 min at 60°C in the absence and presence of subtilisin, respectively. The heterodimeric 67-kDa enzyme has a half-life of 20 min at 60°C in the absence of subtilisin | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
monomeric 30-kDa enzyme and heterodimeric 67000-Da enzyme, in the absence of subtilisin | Bacillus licheniformis |
| 11 | - |
monomeric 30-kDa enzyme, in presence of subtilisin | Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | - |
Bacillus licheniformis |
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