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Literature summary for 2.3.2.2 extracted from

  • Lee, Y.; Chi, M.; Lin, M.; Chen, Y.; Lin, L.; Wang, T.
    Biocatalytic synthesis of gamma-glutamyl-L-leucine, a Kokumi-imparting dipeptide, by Bacillus licheniformis gamma-glutamyltranspeptidase (2018), Food Biotechnol., 32, 130-147 .
No PubMed abstract available
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Application

Application Comment Organism
food industry development of an enzymatic method involving Bacillus licheniformis GGT (BlGGT) for the synthesis of gamma-glutamyl-L-leucine (gamma-Glu-Leu) from glutamine and leucine, since the mass production of Kokumi peptides from natural resources in high purity generally involves several tedious and inefficient operation processes. Parameter optimization of the BlGGT-catalyzed reaction for the biocatalytic synthesis of this Kokumi-imparting molecule Bacillus licheniformis
synthesis high potentiality of BlGGT-catalyzed reaction in the synthesis of gamma-glutamyl compounds in variety of biotechnological and pharmaceutical applications Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis
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 a peptide + a 5-L-glutamyl amino acid
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 ?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-p-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline Bacillus licheniformis 4-nitroaniline + gamma-glutamylglycylglycine
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 ?
a (5-L-glutamyl)-peptide + an amino acid
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 Bacillus licheniformis a peptide + a 5-L-glutamyl amino acid
-
 ?
L-glutamine + L-leucine optimum conditions for a biocatalytic synthesis of gamma-Glu-Leu are determined to be 200 mM glutamine, 200 mM leucine, 50 mM Tris-HCl, pH 9.0, and enzyme BlGGT at a working concentration of 2.0 U/ml. Upon a 5-h incubation of the reaction mixture at 50°C, the product yield reaches approximately 52%. The preparative synthesis of gamma-Glu-Leu by BlGGT is done under optimal conditions at millimolar scale level Bacillus licheniformis gamma-glutamyl-L-leucine + NH3
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 ?
additional information mass spectrometric and NMR analysis of reaction products Bacillus licheniformis ?
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Synonyms

Synonyms Comment Organism
gamma-glutamyltranspeptidase
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 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 active at, inactivation above, profile overview Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
5 12 active at, profile overview Bacillus licheniformis