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Literature summary for 2.3.2.2 extracted from

  • Kimm, S.W.; Kim, E.G.; Park, S.C.
    Partial purification and characterization of gamma-glutamyl transpeptidase from rat kidney (1986), Korean J. Biochem., 18, 135-143.
No PubMed abstract available
Search for more literature for 2.3.2.2

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ no inhibition Rattus norvegicus
glycine strong Rattus norvegicus
additional information no inhibition by phorbol ester, polyamines, cAMP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 L-Glu-4-nitroanilide with 8 mM L-Gly-Gly, kidney enzyme Rattus norvegicus
0.32
-
 L-Glu-4-nitroanilide with 16 mM L-Gly-Gly , kidney enzyme Rattus norvegicus
4
-
 glycylglycine with 0.8 mM L-Glu-4-nitroanilide, kidney enzyme Rattus norvegicus
4.35
-
 glycylglycine with 1.6 mM L-Glu-4-nitroanilide, kidney enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border kidney Rattus norvegicus 5903
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 50000 + 1 * 22000, kidney, SDS-PAGE Rattus norvegicus
50000
-
 1 * 50000 + 1 * 22000, kidney, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
540
-
 kidney Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
 Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamine L-methionine and L-glutamine are best acceptors Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine L-methionine and glutamine are best acceptors Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Rattus norvegicus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 50000 + 1 * 22000, kidney, SDS-PAGE Rattus norvegicus