Literature summary for 2.3.2.2 extracted from

Philips, J.; Dumin, W.; Winefield, C.
Functional characterization of the grapevine gamma-glutamyl transferase/transpeptidase (E.C. 2.3.2.2) gene family reveals a single functional gene whose encoded protein product is not located in either the vacuole or apoplast (2019), Front. Plant Sci., 10, 1402 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene GGT3, Vitis vinifera appears to contain only a single GGT gene, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, transient functional recombinant overexpression of GFP-tagged enzyme in Allium cepa cells and in Nicotiana benthamiana leaves via transformation with Agrobacterium tumefaciens strain GV3101, quantitative RT-PCR exxpression analysis	Vitis vinifera

Cloned (Comment)

Organism

gene GGT3, Vitis vinifera appears to contain only a single GGT gene, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, transient functional recombinant overexpression of GFP-tagged enzyme in Allium cepa cells and in Nicotiana benthamiana leaves via transformation with Agrobacterium tumefaciens strain GV3101, quantitative RT-PCR exxpression analysis

Vitis vinifera

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	the functional VvGGT3 is probably associated with membrane-like structures. VvGGT3-GFP fusion protein does not localize to the vacuole in transformed Nicotiana benthamiana cells	Vitis vinifera	16020	-

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	A0A516F1V8	cv. Sauvignon blanc	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
berry	skin and pulp of mature berries, across whole berry development, VvGGT3 demonstrates a general pattern of increasing in transcript abundance	Vitis vinifera	-
flower	-	Vitis vinifera	-
fruit	-	Vitis vinifera	-
leaf	-	Vitis vinifera	-
additional information	the VvGGT3 transcript is expressed in all tissues tested, i.e. roots, leaf, inflorescences, and tendril, and in the skin, pulp, and seed fractions of mature grape berries. The transcript abundance of VvGGT3 is the lowest in the tendril, followed by the root, with a 1.7 and 1.8fold increase in leaf and inflorescence compared to the tendril, respectively. Focusing on the relative VvGGT3 abundance in the skin, pulp, and seed fractions of mature grape berries, similar levels of transcript is observed in all three berry tissues, with these all being over 7fold higher than the tendril	Vitis vinifera	-
root	-	Vitis vinifera	-
seed	-	Vitis vinifera	-

Synonyms

Synonyms	Comment	Organism
gamma-glutamyl transferase/transpeptidase	-	Vitis vinifera
GGT	-	Vitis vinifera
GGT3	-	Vitis vinifera
VvGGT3	-	Vitis vinifera

Expression

Organism	Comment	Expression
Vitis vinifera	the VvGGT3 transcript in whole berries is upregulated upon Botrytis cinerea infection as well as mechanical damage to leaf tissue. In comparison to Botrytis infection, there is only a mild about 2.0fold increase in the relative transcript abundance of VvGGT3in mechanically wounded leaves compared to the unwounded control leaves at 6 h post wounding beacoming significantly different after 12 h	up

General Information

General Information	Comment	Organism
physiological function	grapevine appears to contain only a single GGT gene, possessing all the key conserved residues needed for GGT activity. VvGGT3 has a proposed role in the formation of precursors of key varietal aromas in wine. These compounds are considered to be derived from the lipoxygenase/hydroperoxide lyase mediated breakdown of linolenic and linoleic C18 fatty acids to produce C6 hexanal and then its derivatives	Vitis vinifera