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3.2.1.91: cellulose 1,4-beta-cellobiosidase (non-reducing end)

This is an abbreviated version!
For detailed information about cellulose 1,4-beta-cellobiosidase (non-reducing end), go to the full flat file.

Word Map on EC 3.2.1.91

Reaction

2 cellohexaose + 2 H2O = 2 cellotriose +

cellotetraose
+
cellobiose

Synonyms

1,4-beta-cellobiohydrolase, 1,4-beta-D-glucan cellobiohydrolase, 1,4-beta-glucan cellobiohydrolase, 1,4-beta-glucan cellobiosidase, avicelase, avicelase II, beta-1,4-glucan cellobiohydrolase, beta-1,4-glucan cellobiosylhydrolase, beta-1,4-glycanase, Beta-1,4-glycanase CEX, Beta-glucancellobiohydrolase, C1 cellulase, CBH, CBH 1, Cbh C, CBH I, CBH Ib, CBH II, CBH IIb, CBH1, CBH2, Cbh6B, Cbh9A, CbhA, CBHI, CBHII, Cbhl.2, CBM3-GH5, CBP120, CBP95, CbsA, Cel5A, Cel6, Cel6A, Cel6B, Cel6C, Cel7A, Cel7D, CelA, CelAB, CelB, CelC7, CelK, cellobiohydrolase, cellobiohydrolase 1, cellobiohydrolase 9A, cellobiohydrolase A, cellobiohydrolase class II, cellobiohydrolase I, cellobiohydrolase II, cellobiohydrolase, exo-, cellobiosidase, cellobiosidase, 1,4-beta-glucan, Celluclast 1.5, Cellulase, cellulase, C1, Cex, Csac_1078, Ex-1, Ex-4, exo-1,4-beta-D-glucan cellobiohydrolase, exo-1,4-beta-D-glucanase, exo-beta-1,4-cellobiohydrolase, exo-beta-1,4-glucan cellobiohydrolase, exo-cellobiohydrolase, exocellobiohydrolase, exocellulase, exocellulase E3, exoglucanase, GH5, glucanase, Huta_2387, More, nonreducing end-acting cellobiohydrolase, oligosaccharide-specific cellobiohydrolase, PSCase, SCO6546, Ta Cel7A, THITE_126432, TTCBH6B

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.91 cellulose 1,4-beta-cellobiosidase (non-reducing end)

Purification

Purification on EC 3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and anion exchange column chromatography
-
ammonium sulfate precipitation and ion exchange chromatography
-
ammonium sulfate precipitation and Superdex 200HR gel filtration
ammonium sulfate precipitation, Mono S column chromatography and Superdex S200 gel filtration
cloned in Escherichia coli
-
DEAE Sepharose column chromatography
-
DEAE Sepharose column chromatography, Sephacryl S-300 gel filtration, and Mono Q column chromatography
-
DEAE-Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration
-
extracellular Cel5A, Cel6A, and Cel7A from culture medium
-
gene cloned in Escherichia coli
-
High Trap DEAE column chromatography
His-Trap column chromatography
HisTrap column chromatography
Thermochaetoides thermophila
HiTrap Q HP column chromatography
-
homogeneity
-
native enzyme from commercial enzyme blend, Spezyme CP by ammonium sulfate fractionation, anion exchange chromatography, and p-aminophenyl-1-thio-beta-D-cellobioside-based affinity chromatography
Ni-IDA resin column chromatography and Superdex 75pg gel filtration
Ni-NTA agarose column chromatography and T7-tag antibody agarose column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography, Source 15S column chromatography, and Superdex 75 gel filtration
-
Ni2+-NTA column chromatography and HiTrap Q HP anion-exchange column chromatography
nickel-NTA resin column chromatography
-
on Ni–NTA column
polypeptide of recombinant Ex-4 from day-3 culture fluid purified by affinity binding toward insoluble phosphoric acid swollen cellulose, to apparent homogeneity
-
Q Sepharose column chromatography, MonoQ column chromatography, and Sephadex G-25 gel filtration
Thermochaetoides thermophila
Q-Sepharose Fast-Flow column chromatography and Mono Q column chromatography
-
recombinant enzyme 2.5fold from Yarrowia lipolytica by ultrafiltration and cation exchange chromatography, recombinant His-tagged enzyme 4.4fold from Pichia pastoris strain X-33 by metal affiity chromatography
recombinant extracellular His6-tagged enzyme from Pichia pastoris strain X-33 culture by nickel affinity chromatography and ultrafiltration
-
recombinant His-tagged enzyme domain constructs from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography and gel filtration
recombinant wild-type Cel6B and mutant enzymes from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography
-
secreted recombinant Ex-1 from Aspergillus oryzae culture fluid by ammonium sulfate fractionation, cellulose adsorption chromatography, and gel filtration
-
Source 15Q column chromatography
-
Source 30Q column chromatography
Superdex 200 gel filtration