Cloned (Comment) | Organism |
---|---|
gene cbh2, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 under the control of the AOX1 promoter, cloning and recombinant expression in Yarrowia lipolytica strain Po1d under the control of the POX2 or TEF promoters, and using either the native or preproLip2 secretion signals. The expression level of cellobiohydrolase from the TEF-preproLip2-cbhII cassette is higher in Yarrowia lipolytica than in Pichia pastoris | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 60000-63000, recombinant enzyme from Yarrowia lipolytica , SDS-PAGE, x * 65000, recombinant His-tagged enzyme from Pichia pastoris , SDS-PAGE | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | P07987 | gene cbh2 | - |
Trichoderma reesei QM9414 | P07987 | gene cbh2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation of the recombinant enzyme does not radically alter the specific activity | Trichoderma reesei |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 2.5fold from Yarrowia lipolytica by ultrafiltration and cation exchange chromatography, recombinant His-tagged enzyme 4.4fold from Pichia pastoris strain X-33 by metal affiity chromatography | Trichoderma reesei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.72 | - |
purified recombinant enzyme from Pichia pastoris, pH 5.0, 50°C | Trichoderma reesei |
2.4 | - |
purified recombinant enzyme from Yarrowia lipolytica, pH 5.0, 50°C | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Avicel PH-101 + H2O | - |
Trichoderma reesei | ? | - |
? | |
Avicel PH-101 + H2O | - |
Trichoderma reesei QM9414 | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
phosphoric acid swollen cellulose + H2O | - |
Trichoderma reesei QM9414 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000-63000, recombinant enzyme from Yarrowia lipolytica , SDS-PAGE, x * 65000, recombinant His-tagged enzyme from Pichia pastoris , SDS-PAGE | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
CBHII | - |
Trichoderma reesei |
cellobiohydrolase II | - |
Trichoderma reesei |
PSCase | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
optimum activity around 55°C, followed by a sharp decline at temperatures above 60°C | Trichoderma reesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzymes, 30 min, good stability up to 40°C | Trichoderma reesei |
60 | - |
purified recombinant enzymes, loss of 50% of the initial activity after 30 min | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Trichoderma reesei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | purified recombinant enzymes, 100% stable in the range pH 5-6, undergoing rapid inactivation at alkaline pH and showing some instability below pH 5 | Trichoderma reesei |