KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
4-nitrophenyl-beta-D-cellobioside | pH 6, 60°C | Acetivibrio thermocellus | |
0.83 | - |
2-chloronitrophenyl-beta-D-lactoside | pH 6, 60°C | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
SDS-PAGE | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloronitrophenyl-beta-D-lactoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-methylumbelliferyl-beta-D-cellobioside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-methylumbelliferyl-beta-D-cellohexaoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-methylumbelliferyl-beta-D-cellopentaoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-methylumbelliferyl-beta-D-cellotetraoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-methylumbelliferyl-beta-D-cellotrioside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-methylumbelliferyl-beta-D-oligoglucosides + H2O | enzyme preferentially cleaves the second or the fourth glycosidic bond, cleaves the second bond between putative sub-sites -2 and -1 or +1 and +2, cleaves the fourth bond between putative sub-sites -1 and +1 | Acetivibrio thermocellus | ? | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Acetivibrio thermocellus | 4-nitrophenol + cellobiose | - |
? | |
cello-oligosaccharides + H2O | natural and derivatized cello-oligosaccharides, H3PO4-swollen cellulose, Avicel, laminarin, lichenan, barley glucan | Acetivibrio thermocellus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2952 | - |
4-nitrophenyl-beta-D-cellobioside | pH 6, 60°C | Acetivibrio thermocellus | |
9108 | - |
2-chloronitrophenyl-beta-D-lactoside | pH 6, 60°C | Acetivibrio thermocellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.4 | stable at 4°C for 48 h | Acetivibrio thermocellus |
6 | - |
complete loss of activity below pH 6 at 60°C for 48 h | Acetivibrio thermocellus |
6 | 8.4 | stable at 60°C for 5 h, stability decreases with incubation time above 5 h | Acetivibrio thermocellus |