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Literature summary for 3.2.1.91 extracted from

  • Bhat, S.; Owen, E.; Bhat, M.K.
    Isolation and characterisation of a major cellobiohydrolase (S8) and a major endoglucanase (S11) subunit from the cellulosome of Clostridium thermocellum (2001), Anaerobe, 7, 171-179.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
4-nitrophenyl-beta-D-cellobioside pH 6, 60°C Acetivibrio thermocellus
0.83
-
2-chloronitrophenyl-beta-D-lactoside pH 6, 60°C Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloronitrophenyl-beta-D-lactoside + H2O
-
Acetivibrio thermocellus ?
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
-
Acetivibrio thermocellus ?
-
?
4-methylumbelliferyl-beta-D-cellohexaoside + H2O
-
Acetivibrio thermocellus ?
-
?
4-methylumbelliferyl-beta-D-cellopentaoside + H2O
-
Acetivibrio thermocellus ?
-
?
4-methylumbelliferyl-beta-D-cellotetraoside + H2O
-
Acetivibrio thermocellus ?
-
?
4-methylumbelliferyl-beta-D-cellotrioside + H2O
-
Acetivibrio thermocellus ?
-
?
4-methylumbelliferyl-beta-D-oligoglucosides + H2O enzyme preferentially cleaves the second or the fourth glycosidic bond, cleaves the second bond between putative sub-sites -2 and -1 or +1 and +2, cleaves the fourth bond between putative sub-sites -1 and +1 Acetivibrio thermocellus ?
-
?
4-nitrophenyl beta-D-cellobioside + H2O
-
Acetivibrio thermocellus 4-nitrophenol + cellobiose
-
?
cello-oligosaccharides + H2O natural and derivatized cello-oligosaccharides, H3PO4-swollen cellulose, Avicel, laminarin, lichenan, barley glucan Acetivibrio thermocellus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2952
-
4-nitrophenyl-beta-D-cellobioside pH 6, 60°C Acetivibrio thermocellus
9108
-
2-chloronitrophenyl-beta-D-lactoside pH 6, 60°C Acetivibrio thermocellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.4 stable at 4°C for 48 h Acetivibrio thermocellus
6
-
complete loss of activity below pH 6 at 60°C for 48 h Acetivibrio thermocellus
6 8.4 stable at 60°C for 5 h, stability decreases with incubation time above 5 h Acetivibrio thermocellus