Information on EC 3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.91
-
RECOMMENDED NAME
GeneOntology No.
cellulose 1,4-beta-cellobiosidase (non-reducing end)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cellulose degradation II (fungi)
-
-
Metabolic pathways
-
-
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan cellobiohydrolase (non-reducing end)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37329-65-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
Allescheria terrestris
-
-
-
Manually annotated by BRENDA team
CBH I, CBH II
-
-
Manually annotated by BRENDA team
synonym Aspergillus nidulans
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains StA, StB, StC, StD, StE, and StF
-
-
Manually annotated by BRENDA team
168
-
-
Manually annotated by BRENDA team
168
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Chaetomium thermophile
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain UV18-25
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Clostridium stercorarium
-
-
-
Manually annotated by BRENDA team
strain 5338
UniProt
Manually annotated by BRENDA team
strain 5338
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
2 forms: I/II
-
-
Manually annotated by BRENDA team
Geotrichum sp.
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain FS010
UniProt
Manually annotated by BRENDA team
strain FS010
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
CBH I
-
-
Manually annotated by BRENDA team
Phytomonas davidi
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
strain CCBAS585
-
-
Manually annotated by BRENDA team
strain CCBAS585
-
-
Manually annotated by BRENDA team
CBH III
-
-
Manually annotated by BRENDA team
strain F7
-
-
Manually annotated by BRENDA team
strain JW20, gene cbhA
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 2-40T
-
-
Manually annotated by BRENDA team
strain 2-40T
-
-
Manually annotated by BRENDA team
2 forms: I/II
-
-
Manually annotated by BRENDA team
bifunctional beta 1,4-endoglucanase/cellobiohydrolase; strain T7902
UniProt
Manually annotated by BRENDA team
bifunctional beta 1,4-endoglucanase/cellobiohydrolase; strain T7902
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain FjSS3-B.1
-
-
Manually annotated by BRENDA team
strain FjSS3-B.1
-
-
Manually annotated by BRENDA team
Torula thermophila
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ALKO2877
-
-
Manually annotated by BRENDA team
CBH I
-
-
Manually annotated by BRENDA team
Cel6A
-
-
Manually annotated by BRENDA team
strain RUT C30
-
-
Manually annotated by BRENDA team
gene cbh2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl beta-cellobioside + H2O
2,4-dinitrophenol + beta-cellobiose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl beta-glucopyranoside + H2O
2,4-dinitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl beta-lactoside + H2O
2,4-dinitrophenol + beta-lactose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl cellobiose + H2O
2,4-dinitrophenol + cellobiose
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl beta-cellobioside + H2O
2-chloro-4-nitrophenol + beta-cellobiose
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl beta-D-lactoside + H2O
2-chloro-4-nitrophenol + lactose
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl beta-lactoside + H2O
2-chloro-4-nitrophenol + beta-lactose
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl lactoside + H2O
2-chloro-4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl-beta-D-cellotrioside + H2O
4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl-beta-D-lactoside
2-chloro-4-nitrophenol + beta-D-lactose
show the reaction diagram
2-chloro-4-nitrophenyl-beta-D-lactoside + H2O
2-chloro-4-nitrophenol + D-lactose
show the reaction diagram
-
-
-
-
?
2-chloro-4-nitrophenyl-beta-lactoside
?
show the reaction diagram
2-chloro-4-nitrophenyl-beta-lactoside + H2O
4-nitrophenol + 2-chloro-beta-lactose
show the reaction diagram
2-chloronitrophenyl-beta-cellotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloronitrophenyl-beta-D-lactoside + H2O
?
show the reaction diagram
-
-
-
-
?
3,4-dinitrophenyl lactoside + H2O
3,4-dinitrophenol + ?
show the reaction diagram
-
-
-
-
?
4-bromophenyl beta-cellobioside + H2O
4-bromophenol + beta-cellobiose
show the reaction diagram
-
-
-
-
?
4-bromophenyl beta-glucopyranoside + H2O
4-bromophenol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
4-bromophenyl beta-lactoside + H2O
4-bromophenol + beta-lactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-cellobioside + H2O
4-methylumbelliferol + cellobiose
show the reaction diagram
-
substrate binding analysis of wild-type enzyme and mutant D274A, mutant D274A binds 4-methylumbelliferyl beta-cellobioside to approximately the same extent as the wild-type, overview
-
-
?
4-methylumbelliferyl beta-D-cellobioside + H2O
4-methylumbelliferol + beta-D-cellobiose
show the reaction diagram
4-methylumbelliferyl beta-D-cellobioside + H2O
4-methylumbelliferone + beta-D-cellobiose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl beta-D-cellobioside + H2O
4-methylumbelliferone + beta-D-cellobioside
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-D-cellobioside + H2O
4-methylumbelliferone + cellobiose
show the reaction diagram
4-methylumbelliferyl beta-D-cellobioside + H2O
?
show the reaction diagram
4-methylumbelliferyl beta-D-lactopyranoside + H2O
4-methylumbelliferone + lactose
show the reaction diagram
4-methylumbelliferyl beta-D-lactopyranoside + H2O
4-methylumbelliferone lactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-D-lactoside + H2O
4-methylumbelliferol + beta-D-lactose
show the reaction diagram
4-methylumbelliferyl beta-lactoside + H2O
4-methylumbelliferol + beta-lactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-lactoside + H2O
4-methylumbelliferone + lactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl lactoside + H2O
4-methylumbelliferone + lactose
show the reaction diagram
4-methylumbelliferyl-beta-D-cellobioside + H2O
4-methylumbelliferol + cellobiose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
?
show the reaction diagram
4-methylumbelliferyl-beta-D-cellohexaoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-cellopentaoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-cellotetraoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-cellotrioside + H2O
?
show the reaction diagram
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferol + beta-D-lactose
show the reaction diagram
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferol + D-lactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + lactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
?
show the reaction diagram
4-methylumbelliferyl-beta-D-oligoglucosides + H2O
?
show the reaction diagram
-
enzyme preferentially cleaves the second or the fourth glycosidic bond, cleaves the second bond between putative sub-sites -2 and -1 or +1 and +2, cleaves the fourth bond between putative sub-sites -1 and +1
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
show the reaction diagram
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
show the reaction diagram
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
4-nitrophenyl beta-D-cellopentaoside + H2O
4-nitrophenol + beta-D-cellopentaose
show the reaction diagram
best substrate
-
-
?
4-nitrophenyl beta-D-cellopentaoside + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-cellotetraoside + H2O
?
show the reaction diagram
4-nitrophenyl beta-D-cellotrioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-cellotrioside + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
0.26% activity compared to 4-nitrophenyl beta-D-cellobioside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
-
0.11% activity compared to 4-nitrophenyl beta-D-cellobioside
-
-
?
4-nitrophenyl beta-D-lactopyranoside + H2O
4-nitrophenol + D-lactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-lactoside + H2O
4-nitrophenol + lactose
show the reaction diagram
-
42.1% activity compared to 4-nitrophenyl beta-D-cellobioside
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannopyranose
show the reaction diagram
-
0.64% activity compared to 4-nitrophenyl beta-D-cellobioside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
show the reaction diagram
-
0.64% activity compared to 4-nitrophenyl beta-D-cellobioside
-
-
?
4-nitrophenyl beta-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-lactoside + H2O
4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-lactoside + H2O
4-nitrophenol + beta-lactose
show the reaction diagram
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
4-nitrophenyl cellopentaoside + H2O
4-nitrophenol + cellobiose + D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl cellopentaoside + H2O
4-nitrophenyl-cellobioside + cellotriose
show the reaction diagram
4-nitrophenyl cellotetraoside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
-
-
-
?
4-nitrophenyl cellotetraoside + H2O
4-nitrophenyl cellobioside + cellobiose
show the reaction diagram
4-nitrophenyl cellotrioside + H2O
4-nitrophenol + cellobiose + D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl D-cellobioside + H2O
4-nitrophenol + D-cellobiose
show the reaction diagram
4-nitrophenyl-beta-D-cellobiose + H2O
4-nitrophenol + D-cellobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-D-cellobioside + H2O
4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-D-lactoside + H2O
4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
alpha-cellobiosyl fluoride + H2O
fluoride + cellobiose
show the reaction diagram
amorphous cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
avicel + H2O
cellobiose
show the reaction diagram
avicel + H2O
cellobiose + ?
show the reaction diagram
avicel + H2O
cellobiose + D-glucose + cellotriose
show the reaction diagram
Avicel PH-101 + H2O
?
show the reaction diagram
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
barley beta-glucan + H2O
?
show the reaction diagram
barley straw + H2O
?
show the reaction diagram
-
-
-
-
?
beta-cellobiosyl fluoride + H2O
fluoride + cellobiose
show the reaction diagram
beta-glucan + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
?
show the reaction diagram
carboxymethylcellulose + H2O
?
show the reaction diagram
carboxymethylcellulose + H2O
cellobiose
show the reaction diagram
carboxymethylcellulose + H2O
cellobiose + ?
show the reaction diagram
carboxymethylcellulose + H2O
glucose + cellobiose + cellutetraose
show the reaction diagram
cello-oligosaccharides + H2O
?
show the reaction diagram
-
natural and derivatized cello-oligosaccharides, H3PO4-swollen cellulose, Avicel, laminarin, lichenan, barley glucan
-
-
?
cellobiose + H2O
?
show the reaction diagram
cellodextrin + H2O
?
show the reaction diagram
cellodextrins + H2O
cellobiose + glucose + cellotriose
show the reaction diagram
-
-
-
?
cellohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
cellotriose + cellobiose + cellotetraose
show the reaction diagram
celloligosaccharides + H2O
cellobiose + H2O
show the reaction diagram
-
-
-
?
cellononaose + H2O
?
show the reaction diagram
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
cellobiose + ?
show the reaction diagram
cellopentaose + H2O
cellobiose + cellotriose
show the reaction diagram
-
good substrate
-
-
?
cellopentaose + H2O
cellobiose + D-glucose
show the reaction diagram
-
-
-
?
cellopentaose + H2O
cellobiose + glucose + cellotriose
show the reaction diagram
cellopentaose + H2O
cellotriose + cellobiose
show the reaction diagram
cellotetraose + H2O
2 cellobiose
show the reaction diagram
cellotetraose + H2O
?
show the reaction diagram
cellotetraose + H2O
cellobiose + ?
show the reaction diagram
cellotetraose + H2O
cellobiose + D-glucose + cellotriose
show the reaction diagram
cellotriitol + H2O
cellobiose + sorbitol
show the reaction diagram
-
-
-
?
cellotriose + H2O
?
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
cellobiose + glucose
show the reaction diagram
cellotriose + H2O
glucose + cellobiose
show the reaction diagram
-
-
-
?
cellulose + H2O
?
show the reaction diagram
cellulose + H2O
beta-cellobiose
show the reaction diagram
cellulose + H2O
beta-D-cellobiose
show the reaction diagram
-
from cotton
-
-
?
cellulose + H2O
cellobiose
show the reaction diagram
cellulose + H2O
cellobiose + ?
show the reaction diagram
cellulose + H2O
cellobiose + cellohexaose
show the reaction diagram
hydrolyzes Avicel and filter paper, efficiency of hydrolysis increases in the following order: Gh9, Gh9-Fn31,2, Gh9-Fn31,2-CBDIII
-
-
?
cellulose + H2O
cellobiose + D-glucose + cellotriose
show the reaction diagram
cellulose + H2O
cellobiose + glucose + cellotriose
show the reaction diagram
corn stover + H2O
cellobiose + ?
show the reaction diagram
-
-
-
?
cotton + H2O
?
show the reaction diagram
-
-
-
-
?
crystalline cellulose + H2O
?
show the reaction diagram
-
-
-
?
crystalline cellulose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
filter paper + H2O
?
show the reaction diagram
highly crystalline cellulose + H2O
cellobiose
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
lichenan + H2O
?
show the reaction diagram
methylumbelliferyl-lactoside + H2O
methylumberlliferone + lactose
show the reaction diagram
-
-
-
-
?
microcrystalline cellulose Ibeta + H2O
?
show the reaction diagram
-
enzyme-substrate complex, computational simulations and molecular dynamics, modelling of the enzyme interacting with a model segment of a cellulose microfibril, detailed overview
-
-
?
nanocellulose + H2O
cellobiose + ?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-cellobioside + H2O
p-nitrophenol + beta-D-cellobiose
show the reaction diagram
p-nitrophenyl beta-D-cellobioside + H2O
p-nitrophenol + cellobiose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
p-nitrophenol + beta-D-cellobiose
show the reaction diagram
p-nitrophenyl-beta-D-cellobioside + H2O
p-nitrophenol + cellobiose
show the reaction diagram
p-nitrophenyl-lactoside + H2O
p-nitrophenol + lactose
show the reaction diagram
phosphoric acid swollen cellulose + H2O
?
show the reaction diagram
phosphoric acid swollen cellulose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
phosphoric acid swollen cellulose + H2O
cellobiose + cellotriose + ?
show the reaction diagram
phosphoric acid swollen cellulose + H2O
cellobiose + D-glucose + cellotriose
show the reaction diagram
phosphoric acid-swollen Avicel + H2O
cellobiose
show the reaction diagram
-
low specific activity
-
-
?
phosphoric acid-swollen cellulose + H2O
cellobiose
show the reaction diagram
sigmacell 20 + H2O
?
show the reaction diagram
sodium hydroxide pretreated corn stover + H2O
?
show the reaction diagram
sulforhodamine-conjugated cellohexaose + H2O
cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
?
wheat straw + H2O
?
show the reaction diagram
wheat straw + H2O
cellobiose + ?
show the reaction diagram
-
-
-
-
?
xylan + H2O
?
show the reaction diagram
xylan + H2O
xylobiose + xylotetraose + xylohexaose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellulose + H2O
?
show the reaction diagram
cellulose + H2O
beta-cellobiose
show the reaction diagram
cellulose + H2O
cellobiose
show the reaction diagram
cellulose + H2O
cellobiose + ?
show the reaction diagram
-
-
-
-
?
phosphoric acid swollen cellulose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
role in the cellusome, an active cellulase system
-
-
-